Robust Dopaminergic Differentiation and Enhanced LPS-Induced Neuroinflammatory Response in Serum-Deprived Human SH-SY5Y Cells: Implication for Parkinson’s Disease

Niaz, Aram; Karunia, Jocelyn; Mandwie, Mawj; Keay, Kevin A.; Musumeci, Giuseppe; Al-Badri, Ghaith; Castorina, Alessandro

doi:10.1007/s12031-020-01678-6

Cited by 8 publications

(5 citation statements)

References 65 publications

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“…Especially during the implementation of Large-Scale Social Restrictions (PSBB) and the Enactment of Community Activity Restrictions (PPKM), where people are asked to be able to carry out activities and remain productive at home, so that internet services become a new staple in the pandemic period. The results of this study support an article written by (Niaz et al, 2021), where telecommunication companies such as Telkom recorded an increase in customer numbers and revenue in 2020 compared to 2019.…”

Section: First Hypothesissupporting

confidence: 85%

Analysis of Abnormal Return and Volume Activity of Telecommunications Sector Shares in Indonesia Before and After Covid-19

Sumadi

Tamara

Tama

et al. 2021

SLJIL

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The study intends to investigate if there are significant differences in the abnormal return and volume activity of telecommunication companies 90 exchange days before and 90 exchange days after the announcement of the first Covid-19 infection in Indonesia with the Event Study method. The strategies used in this study are quantitative methods with statistical description analysis using different t-test with paired samples. The samples used in this study are EXCL, ISAT, and TLKM companies included in the telecommunications infrastructure sector and listed on the Indonesia Stock Exchange (IDX). The research test results showed that there was no significant difference in the abnormal return and trading volume activity of telecommunication companies in Indonesia. It proves that telecommunication companies' performance in Indonesia is not affected by the Covid-19 pandemic, because the demand for internet and telecommunication services has become the basic need for people to be able to maintain their productivity at home.

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Section: First Hypothesissupporting

confidence: 85%

Analysis of Abnormal Return and Volume Activity of Telecommunications Sector Shares in Indonesia Before and After Covid-19

Sumadi

Tamara

Tama

et al. 2021

SLJIL

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“…Human dopaminergic neuronal SH-SY5Y cell line (less than ten passages) was purchased from the Chinese Academy of Medical Sciences (Shanghai, China), cultured using 10% fetal bovine serum of DMEM (Gibco, USA) at 37°C 5% CO 2 , and sub-cultured following the cell density reached 90% confluence. To figure out the effect of NEAT1 on PD, a model of PD mice in vitro was construed by administering varying concentrations of MPP+ treated SH-SY5Y cells and cultivating (0, 100, 200, 400, and 800 μmol/L) for 24 h [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

lncRNA NEAT1 promotes autophagy of neurons in mice by impairing miR-107-5p

2022

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This work focused on the exploration of NEAT1 in Parkinson’s disease (PD) and aimed to explore its effects on PD and related molecular mechanisms. Two experimental models were initially constructed, including MPTP-induced mice in vivo and the MPP+-induced SH-SY5Y cell line in vitro. Immunofluorescence assays were conducted to determine the TH+ positive cell rate. Pole tests and rotarod tests were also performed for the visualization of behavioral changes in mice. Cellular apoptosis was determined using MTT and flow cytometry assays. Changes in the number of autophagosomes were obtained under a transmission electron microscope. The content of dopamine was confirmed by high performance liquid chromatography. The targeted interrelationship between miR-107-5p and NEAT1 was clarified via dual-luciferase reporter gene assays. Meanwhile, mRNA and protein expressions were also detected using qRT-PCR and Western blot respectively. Furthermore, the level of NEAT1 was positively correlated with MPP+ concentration. Interfering with NEAT1 in the present study promoted cellular proliferation and mediated SH-SY5Y cell apoptosis and autophagy treated with MPP+. An increase was discovered in TH positive neurons and suppressive autophagy in PD mice. miR-107-5p was then considered as a NEAT1 putative target involving apoptosis and autophagy of SH-SY5Y cells. Interfering with NEAT1 efficiently facilitated the viability of SH-SY5Y cells and drastically suppressed autophagy and apoptosis of PD mice induced by MPTP- via elevating miR-107-5p level, which indicated that lncRNA NEAT1 acted as a latent therapeutic factor for PD treatment.

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“…Asimismo, la administración de AbA tampoco ha mostrado ningún efecto en dicha activación, ni tampoco parece funcionar como estímulo diferenciador neuronal en dicha línea celular. En estudios previos, la administración de lps sobre la línea celular SH-SY5Y provoca la liberación de citoquinas proinflamatorias y el aumento del estrés oxidativo (Zhang et al 2020;Niaz et al 2021). En el caso de estudios con animales, se ha reportado que la administración sistémica de lps genera una disminución en la fosforilación de los residuos de tirosina del IRS1 y el aumento de las serinas, comprometiendo así la vía IRS1/PI3K/AKT en el cerebro de estos animales.…”

Section: Discusión Y Conclusionesunclassified

“…El lps es reconocido por los receptores toll-like receptors type 4 (TLR4) por parte de las células gliales y las neuronas, promoviendo la secreción de citoquinas proinflamatorias (Okun et al 2011). Los efectos de lps se han estudiado de forma extensiva en la línea celular de neuroblastoma SH-SY5Y, modelo de neuronas catecolaminérgicas Niaz et al 2021). No obstante, el efecto que puede ejercer el lps sobre la cascada de señalización de la insulina en las neuronas no ha sido estudiado hasta la fecha.…”

Section: Introductionunclassified

Efectos neuroprotectores del ácido abscísico sobre la línea celular SH-SY5Y en condiciones proinflamatorias

Iván¹,

Sánchez-Pérez²

2022

AgoraSalut

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El lipopolisacárido (lps), es un estímulo proinflamatorio para las neuronas, mientras que el ácido abscísico (AbA), es una fitohormona que ha mostrado efectos protectores ante la neuroinflamación, el deterioro cognitivo y la resistencia a insulina periférica, además de propiedades como estímulo diferenciador de células de glioma humano. Por ello, los objetivos de este estudio se centraron en evaluar (1) el efecto de la administración de lps sobre la señalización de insulina, (2) la capacidad neuroprotectora de AbA ante esta situación y (3) el efecto de AbA como estímulo diferenciador neuronal. Para ello, se administró de forma aguda (24h) y de forma crónica (5 días) lps y AbA sobre la línea celular de neuroblastoma SH-SY5Y, y mediante Western blot se cuantificó la activación de proteínas de señalización de la insulina. En cuanto al estudio de diferenciación, se trataron las células SH-SY5Y con AbA (5 días) y posteriormente se analizó de forma cualitativa el estado de diferenciación neuronal. Nuestros resultados mostraron que la administración de lps y AbA, aguda y crónicamente, no ejerce efectos sobre la activación de las proteínas implicadas en la señalización de la insulina. Asimismo, la administración de AbA tampoco mostró cambios en el fenotipo neuronal indiferenciado. En conclusión, nuestro estudio in vitro sugiere que el lps no ejerce como estímulo dañino ante la señalización de insulina, y que AbA no contiene propiedades de diferenciación neuronal en las células SH-SY5Y.

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Robust Dopaminergic Differentiation and Enhanced LPS-Induced Neuroinflammatory Response in Serum-Deprived Human SH-SY5Y Cells: Implication for Parkinson’s Disease

Cited by 8 publications

References 65 publications

Analysis of Abnormal Return and Volume Activity of Telecommunications Sector Shares in Indonesia Before and After Covid-19

Analysis of Abnormal Return and Volume Activity of Telecommunications Sector Shares in Indonesia Before and After Covid-19

lncRNA NEAT1 promotes autophagy of neurons in mice by impairing miR-107-5p

Efectos neuroprotectores del ácido abscísico sobre la línea celular SH-SY5Y en condiciones proinflamatorias

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