RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

“…In some cases, mutations in ace1 explain better than mutations in ace2 the altered AChE activity observed in association with insecticide resistance (Kono and Tomita, 2006). In Blattella germanica, specific interference of Bgace1 and Bgace2 genes demonstrates that BgAChE1 is responsible for at least 65e75% of the total AChE activity detected in nerve tissues, and specific interference of Bgace1 produces an increased mortality in insects when treated with insecticides that is not produced after specific interference of Bgace2 (Revuelta et al, 2009). Finally, studies on tissue distribution of Bgace1 and Bgace2 transcripts (Kim et al, 2006b), and the abundance of BgAChE1 in comparison with BgAChE2 using specific antibodies (Kim et al, 2010), suggest the major role of BgAChE1 in postsynaptic transmission.…”

Distinct contributions of A314S and novel R667Q substitutions of acetylcholinesterase 1 to carbofuran resistance of Chilo suppressalis Walker

“…1 To date, two AChE genes (ace), ace1 and ace2, which encode AChE1 and AChE2, have been identified in most insects. 2 A recent study conducted using native gel electrophoresis and western blotting confirmed that AChE1 was the main form within the head extract of 67 out of 100 insect species examined. 3 AChE, which plays a critical role in the nervous system, is the main target of organophosphate (OP) and carbamate (CB) compounds for insect pest control.…”

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“…Diverse works correlate the resistance to pesticides with the increase of enzymatic activity, attributing it to punctual mutations, genic duplications and mutations in the target molecule (Mutero et al, 1994;Villatte et al, 2000;Vontas et al, 2001;Vontas et al, 2002;Ortelli et al, 2003;Menozzi et al, 2004;Revuelta et al, 2009;Ying et al, 2013;Chang et al, 2014;Zhao et al, 2014;Xi et al, 2015).…”

“…Revista Bio Ciencias 4(6), Article ID 04.06.01 December 2017 presentes en A. gambiae (Weill et al, 2002;Fournier, 2005), sin embargo, en D. melanogaster la AChE es codificada úni-camente por ace2 (Fournier et al, 1993;Weill et al, 2002;Huchard et al, 2006); estudios en otros insectos que poseen ambos genes, sugieren que ace1 es el gen responsable de una mayor actividad de la AChE (Revuelta et al, 2009;Revuelta et al, 2011).…”