RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato

Ewas, Mohamed; Gao, Yangqiang; Ali, Farhan; Nishawy, Elsayed; Shahzad, Raheel; Subthain, Hizar; Amar, Mohamed Hamdy; Martin, Cathie; Luo, Jie

doi:10.1016/j.scib.2017.03.018

Cited by 25 publications

(23 citation statements)

References 55 publications

(64 reference statements)

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“…populiperda infection [42]. Similarly, in tomato, overexpression of SlMX1 increased the plant tolerance to P. infestans and Botrytis cinerea, which caused late blight and gray mold, respectively [43]. In this study, one DXS synthase gene MELO3C014965 was significantly up-regulated in ZQK9 but down-regulated in E31 at 5 dpi.…”

Section: Biosynthesis Of Secondary Metabolitessupporting

confidence: 47%

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Wang

Zhao

et al. 2020

PLoS ONE

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Phytophthora blight caused by Phytophthora capsici is a devastating disease for melon plant. However, the underlying resistance mechanisms are still poorly understood. In this study, the transcriptome differences between the resistant ZQK9 and susceptible E31 at 0, 3, and 5 days post-inoculation (dpi) were identified by RNA-seq. A total of 1,195 and 6,595 differentially expressed genes (DEGs) were identified in ZQK9 and E31, respectively. P. capsici infection triggered massive transcript changes in the inoculated tissues. Genes related to plant defense responses were activated, which was reflected by a lot of up-regulated DEGs involved in pathogenesis-related (PR) genes, hormones biosynthesis and signal transduction, secondary metabolites biosynthesis and cell wall modification in resistant ZQK9. The dataset generated in this study may provide a basis for identifying candidate resistant genes in melon against P. capsici and lay a foundation for further research on the molecular mechanisms.

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Section: Biosynthesis Of Secondary Metabolitessupporting

confidence: 47%

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Wang

Zhao

et al. 2020

PLoS ONE

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“…However, the content and distribution of individual secondary metabolites vary greatly among plant genotypes [41]. Here, the important roles of zeatin, sesquiterpenoid [42] and triterpenoid [43], carotenoid and diterpenoid [44], monoterpenoid [45] and phenylpropanoid [46] were also highlighted by the existence of a large number of upregulated genes involved in the synthesis of these chemicals in Caoyuan No.4, which indicated that thrips defense in Caoyuan No.4 was related to the existence of certain secondary metabolites. It is well known that the interaction of plant-pathogen and plant-insects share some strategies.…”

Section: Discussionmentioning

confidence: 91%

“…Furthermore, 117 genes shared in both S_CK vs S_T group and S_CK vs R_CK group were mainly related to secondary metabolism, fatty acid metabolism, amino acid metabolism, rust resistance kinase, WRKY transcription factor and nodule lectin. Thus, a majority of genes were probably associated with the plant induced defense to herbivorous insects [40][41][42][43][44][45][46][47][48][49][50][51][52]. In addition, the other 2087 DEGs only expressed in Caoyuan No.4 were mainly constitutive defensive genes, which are involved in energy metabolism, carbohydrate metabolism and lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis between thrips-resistant and thrips-susceptible alfalfa (Medicago sativa L.)

Zhang¹,

Buhe²,

Wang³

et al. 2020

Preprint

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Background: Thrips (Thysanoptera: Thripidae) are major insect pests on alfalfa and result in decreased plant nutrients and growth, low yields and even plant death. In our previous studies, an alfalfa variety (Caoyuan No.4) with high thrips resistance was bred through consecutive field recurrent selection. In order to better understand the genetic and molecular mechanisms of thrips resistance in Caoyuan No.4, RNA-Sequencing was employed using the thrips-resistant alfalfa accession (Caoyuan No.4) and a thrips-susceptible alfalfa accession (Caoyuan No.2), each with and without thrips infestation.Results: There were 851 genes constitutively upregulated and 434 genes downregulated in Caoyuan No.4 compared to Caoyuan No.2 without thrips infestation. The upregulated genes were mainly involved in primary metabolism such as energy metabolism and carbohydrate metabolism, lipid metabolism and certain secondary metabolites, while the downregulated genes were mainly related to plant-pathogen interaction. In addition, very few DEGs (only 13) were detected in Caoyuan No.4 after thrips stress, but a total of 3326 contigs DEGs were detected in Caoyuan No.2 after thrips stress. The upregulated genes in Caoyuan No.2 after stress were mainly involved in isoflavonoid biosynthesis, proteasome, amino sugar and nucleotide sugar metabolism, flavonoid biosynthesis as well as plant-pathogen interaction. Moreover, 117 genes that were shared in both the S_CK vs S_T group and S_CK vs R_CK group were divided into 6 clusters, which are mainly involved in secondary metabolism, fatty acid metabolism, amino acid metabolism, rust resistance kinase, WRKY transcription factor and nodule lectin.Conclusion: Both constitutive defensive genes and potential induced defensive genes were detected in the defense of Caoyuan No.4. That two distinct kinds of defensive genes — constitutive defensive genes and induced defensive genes — can be simultaneously activated and thus potentially enhance plant protection against insects attacks is a signiﬁcant finding for plant resistance breeders.

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“…Based on RNA-seq technology, genome-wide analysis of gene expression was performed on the ants of Pro-0 and Pro-30 groups to identify the responsive genes and biological pathways influenced by the accumulation of L-proline (Dataset S3 online) [35]. Rearing the ants on L-proline-augmented diets, 1351 genes were up-regulated (Dataset S4 online), and 2166 genes (Dataset S5 online) were down-regulated ( Fig.…”

Section: L-proline Feeding Induces Gene Expression Responsesmentioning

confidence: 99%

L-proline feeding for augmented freeze tolerance of Camponotus japonicus Mayr

Dou

Sun

et al. 2019

Science Bulletin

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RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato

Cited by 25 publications

References 55 publications

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Comparative transcriptome analysis between thrips-resistant and thrips-susceptible alfalfa (Medicago sativa L.)

L-proline feeding for augmented freeze tolerance of Camponotus japonicus Mayr

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