L-proline feeding for augmented freeze tolerance of Camponotus japonicus Mayr

Dou, Mengjia; Li, Yazhou; Sun, Zejun; Li, Lei; Rao, Wei

doi:10.1016/j.scib.2019.09.028

Cited by 19 publications

(13 citation statements)

References 37 publications

(46 reference statements)

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“…Some insects seasonally accumulate high concentrations of free amino acids, especially proline [37][38][39][40]. The cryoprotective effects of proline are well documented [19,20,[41][42][43][44][45] but the metabolic origin of proline in cold-hardy insects was not previously studied. We speculated that collagens in the extracellular matrix (ECM) may represent macromolecular depots of relatively 'dispensable' proteins from which proline, and other amino acids, can be obtained upon need [20,40].…”

Section: Introductionmentioning

confidence: 99%

Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costata

et al. 2022

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Many cold-acclimated insects accumulate high concentrations of low molecular weight cryoprotectants (CPs) in order to tolerate low subzero temperatures or internal freezing. The sources from which carbon skeletons for CP biosynthesis are driven, and the metabolic reprogramming linked to cold acclimation, are not sufficiently understood. Here we aim to resolve the metabolism of putative CPs by mapping relative changes in concentration of 56 metabolites and expression of 95 relevant genes as larvae of the drosophilid fly, Chymomyza costata transition from a freeze sensitive to a freeze tolerant phenotype during gradual cold acclimation. We found that C. costata larvae may directly assimilate amino acids proline and glutamate from diet to acquire at least half of their large proline stocks (up to 55 µg per average 2 mg larva). Metabolic conversion of internal glutamine reserves that build up in early diapause may explain the second half of proline accumulation, while the metabolic conversion of ornithine and the degradation of larval collagens and other proteins might be two additional minor sources. Next, we confirm that glycogen reserves represent the major source of glucose units for trehalose synthesis and accumulation (up to 27 µg per larva), while the diet may serve as an additional source. Finally, we suggest that interconversions of phospholipids may release accumulated glycero-phosphocholine (GPC) and -ethanolamine (GPE). Choline is a source of accumulated methylamines: glycine-betaine and sarcosine. The sum of methylamines together with GPE and GPC represents approximately 2 µg per larva. In conclusion, we found that food ingestion may be an important source of carbon skeletons for direct assimilation of, and/or metabolic conversions to, CPs in a diapausing and cold-acclimated insect. So far, the cold-acclimation- linked accumulation of CPs in insects was considered to be sourced mainly from internal macromolecular reserves.

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Section: Introductionmentioning

confidence: 99%

Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costata

et al. 2022

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“…Thus, it was hypothesized that the glycopeptide with cell membrane stabilization could assist a better efficiency of RBC cryopreservation, combining with the IRI activity of Tre . There was also an alternative protocol integrating membrane permeable l -proline and Tre to cryoprotect RBCs. , Herein, the cryopreservation of sheep RBCs in the glycopeptide with Tre solution was implemented via a rapid freezing and thawing protocol. Briefly, a mixture suspension of RBCs and glycopeptide with Tre in PBS was rapidly plunged into liquid nitrogen for storage overnight followed by a rapid thawing process at 37 °C.…”

Section: Resultsmentioning

confidence: 99%

Membrane Stabilization of Poly(ethylene glycol)-b-polypeptide-g-trehalose Assists Cryopreservation of Red Blood Cells

Liu

Zhang

et al. 2020

ACS Appl. Bio Mater.

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As alternative cryoprotectants of conventional organic glycerol, biocompatible synthetic glycopeptides that can assist cryopreservation of red blood cells (RBCs) are proposed in this study. A series of glycopeptides are synthesized via ring-opening polymerization of N-carboxyanhydrides of lysine, aspartic acid, and phenylalanine initiated by poly(ethylene glycol)-NH2 and followed by chemical tethering carboxylated trehalose to the pendant amino moieties. The synthetic glycopeptides demonstrate distinguished features of low cytotoxicity, low hemolysis, and cell membrane stabilization. The specifically designed glycopeptides enhance cryosurvival recovery of sheep RBCs up to 87.3 ± 0.3% at pH 6.0 and 86.5 ± 0.3% at pH 7.4 together with trehalose during cryopreservation. The synergistic cryopreservation of RBCs is achieved via membrane stabilization of the glycopeptide and ice recrystallization inhibition of trehalose during freezing and thawing. Molecular dynamics simulation indicates that the glycopeptides enhance membrane stabilization at −196 °C by tuning water diffusion. This work provides a potential option of highly efficient and glycerol-free RBC cryopreservation by combination of synthetic glycopeptides and trehalose, which would inspire design and utilization of different mechanisms for the effective cryopreservation of cells or tissues.

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“…It was believed that proline reduced protein unfolding and cell membrane fusion, thus improving the survival rate in cold environments. In another study, Dou et al [56] fed proline to Camponotus japonicus Mayr (insect) for 5 days. When the insects were cooled to -26.66°C, their survival rate was over twice that of the control group.…”

Section: Prolinementioning

confidence: 99%

A Review of the Material Characteristics, Antifreeze Mechanisms, and Applications of Cryoprotectants (CPAs)

Liu

Pan

Liu

et al. 2021

Journal of Nanomaterials

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Cryopreservation has been a key technology in medical science, food preservation, and many other fields. In a freezing process, the formation of ice crystals may cause significant damage to the frozen samples. In order to reduce the damage, many cryoprotectants (CPAs) have been developed and added in cryopreservation processes for reduced ice volume, decreased ice size, proper ice shaping, and cell protection. According to the material characteristics, the CPAs are either impermeable (i.e., antifreeze protein, polyampholytes, and polyvinyl alcohol) or permeable (i.e., dimethyl sulfoxide, proline, and glycerol) to cell membranes. The typical CPAs are introduced in this work with their material characteristics, antifreeze mechanisms, and applications. Antifreeze mechanisms for different CPAs involve molecular adsorption on the ice surface, hydrogen bonding to ice, bending the ice surface, lowering the freezing point, inhibiting ice recrystallization, protecting cell membranes, reducing dehydration of cells, and breaking hydrogen bonds among ice crystals to reduce the size of ice crystals. In practice, different CPAs can be used together with their cryopreservation properties functioning synergetically. This study reviews the recent applications of CPAs in food, biology and medicine, and agriculture. Future works for CPAs are suggested in improving efficiency, revealing mechanisms, broadening application, and finding new CPAs.

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L-proline feeding for augmented freeze tolerance of Camponotus japonicus Mayr

Cited by 19 publications

References 37 publications

Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costata

Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costata

Membrane Stabilization of Poly(ethylene glycol)-b-polypeptide-g-trehalose Assists Cryopreservation of Red Blood Cells

A Review of the Material Characteristics, Antifreeze Mechanisms, and Applications of Cryoprotectants (CPAs)

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