Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Wang, Pingyong; Wu, Haibo; Zhao, Guangwei; He, Yue; Kong, Weihu; Zhang, Jian; Liu, Shuimiao; Liu, Mengli; Hu, Keyun; Liu, Lifeng; Xu, Yong; Xu, Zhigao

doi:10.1371/journal.pone.0227284

Cited by 24 publications

(24 citation statements)

References 43 publications

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“…Several comparative transcriptomic studies have been reported in melon [ 75 – 77 ], but few studies have focused exclusively on expression profiling of R -genes against phytopathogenic agents in melon. For example, RNA-seq assessment of the changes in transcript levels at different time points in Phytophthora capsici -inoculated tissues of resistant and susceptible melon genotypes provided a basis for identifying candidate resistant genes [ 78 ]. Comparative transcriptome analysis identified ten genes that were differentially expressed in resistant and susceptible cultivars of melon in response to powdery mildew [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization, identification and expression profiling of genome-wide R-genes in melon and their putative roles in bacterial fruit blotch resistance

et al. 2020

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Background: Bacterial fruit blotch (BFB), a disease caused by Acidovorax citrulli, results in significant economic losses in melon. The causal QTLs and genes for resistance to this disease have yet to be identified. Resistance (R)genes play vital roles in resistance to plant diseases. Since the complete genome sequence of melon is available and genome-wide identification of R-genes has been performed for this important crop, comprehensive expression profiling may lead to the identification of putative candidate genes that function in the response to BFB. Results: We identified melon accessions that are resistant and susceptible to BFB through repeated bioassays and characterized all 70 R-genes in melon, including their gene structures, chromosomal locations, domain organizations, motif distributions, and syntenic relationships. Several disease resistance-related domains were identified, including NBS, TIR, LRR, CC, RLK, and DUF domains, and the genes were categorized based on the domains of their encoded proteins. In addition, we profiled the expression patterns of the genes in melon accessions with contrasting levels of BFB resistance at 12 h, 1 d, 3 d, and 6 d after inoculation with A. citrulli. Six Rgenes exhibited consistent expression patterns (MELO3C023441, MELO3C016529, MELO3C022157, MELO3C022146, MELO3C025518, and MELO3C004303), with higher expression levels in the resistant vs. susceptible accession. Conclusion: We identified six putative candidate R-genes against BFB in melon. Upon functional validation, these genes could be targeted for manipulation via breeding and biotechnological approaches to improve BFB resistance in melon in the future.

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Section: Discussionmentioning

confidence: 99%

Characterization, identification and expression profiling of genome-wide R-genes in melon and their putative roles in bacterial fruit blotch resistance

et al. 2020

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“…Several comparative transcriptomic studies have been reported in melon [75][76][77], but few studies have focused exclusively on expression pro ling of R-genes against phytopathogenic agents in melon. For example, RNA-seq assessment of the changes in transcript levels at different time points in Phytophthora capsici-inoculated tissues of resistant and susceptible melon genotypes provided a basis for identifying candidate resistant genes [78]. Comparative transcriptome analysis identi ed ten genes that were differentially expressed in resistant and susceptible cultivars of melon in response to powdery mildew [79].…”

Section: Discussionmentioning

confidence: 99%

Characterization, Identification and Expression Profiling of Genome-Wide R-Genes in Melon and Their Putative Roles in Bacterial Fruit Blotch Resistance

Islam

Hossain

Jesse

et al. 2020

Preprint

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Abstract Background: Bacterial fruit blotch (BFB), a disease caused by Acidovorax citrulli, results in significant economic losses in melon. The causal QTLs and genes for resistance to this disease have yet to be identified. Resistance (R)-genes play vital roles in resistance to plant diseases. Since the complete genome sequence of melon is available and genome-wide identification of R-genes has been performed for this important crop, comprehensive expression profiling may lead to the identification of putative candidate genes that function in the response to BFB.Results: We identified melon accessions that are resistant and susceptible to BFB through repeated bioassays and characterized all 70 R-genes in melon, including their gene structures, chromosomal locations, domain organizations, motif distributions, and syntenic relationships. Several disease resistance-related domains were identified, including NBS, TIR, LRR, CC, RLK, and DUF domains, and the genes were categorized based on the domains of their encoded proteins. In addition, we profiled the expression patterns of the genes in melon accessions with contrasting levels of BFB resistance at 12 h, 1 d, 3 d, and 6 d after inoculation with A. citrulli. Six R-genes exhibited consistent expression patterns (MELO3C023441, MELO3C016529, MELO3C022157, MELO3C022146, MELO3C025518, and MELO3C004303), with higher expression levels in the resistant vs. susceptible accession. Conclusion: We identified six putative candidate R-genes against BFB in melon. Upon functional validation, these genes could be targeted for manipulation via breeding and biotechnological approaches to improve BFB resistance in melon in the future.

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“…www.nature.com/scientificreports/ Discussion P. capsici is a destructive pathogen which has caused widespread and devastating damage to the melon industry 4 . In our previous study, one melon inbred line, ZQK9, was identified as highly resistant to P. capsici 14 . Using ZQK9 and another highly susceptible line E31 as the paternal and maternal lines, we constructed the F 1 , F 2 , and BC 1 populations and found the resistance in ZQK9 was controlled by a dominant gene, tentatively named MePhyto.…”

Section: Expression Analysis Of Genes In Target Region the Expressiomentioning

confidence: 99%

Genetic mapping and candidate gene analysis for melon resistance to Phytophthora capsici

Wang

Zhao

et al. 2020

Sci Rep

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Phytophthora blight is one of the most serious diseases affecting melon (Cucumis melo) production. Due to the lack of highly resistant germplasms, the progress on disease-resistant research is slow. To understand the genetics of melon resistance to Phytophthora capsici, an F2 population containing 498 individuals was developed by crossing susceptible line E31 to highly resistant line ZQK9. Genetic analysis indicated that the resistance in ZQK9 was controlled by a dominant gene, tentatively named MePhyto. Through bulked-segregant analysis (BSA-Seq) and chromosome walking techniques, the MePhyto gene was mapped to a 52.44 kb interval on chromosome 12. In this region, there were eight genes and their expression patterns were validated by qRT-PCR. Among them, one wall-associated receptor kinase (WAK) gene MELO3C002430 was significantly induced in ZQK9 after P. capsici inoculation, but not in E31. Based on the non-synonymous mutation site in MELO3C002430, a cleaved amplified polymorphic sequence (CAPS) marker, CAPS2430, was developed and this maker was co-segregated with MePhyto in both F2 population and a collection of 36 melon accessions. Thus MELO3C002430 was considered as the candidate gene and CAPS2430 was a promising marker for marker-assisted selection (MAS) in breeding. These results lay a foundation for revealing the resistance mechanism of melon to P. capsici.

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Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Cited by 24 publications

References 43 publications

Characterization, identification and expression profiling of genome-wide R-genes in melon and their putative roles in bacterial fruit blotch resistance

Characterization, identification and expression profiling of genome-wide R-genes in melon and their putative roles in bacterial fruit blotch resistance

Characterization, Identification and Expression Profiling of Genome-Wide R-Genes in Melon and Their Putative Roles in Bacterial Fruit Blotch Resistance

Genetic mapping and candidate gene analysis for melon resistance to Phytophthora capsici

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