RNA editing in Newcastle disease virus

Steward, M. W.; Vipond, Ian B.; Millar, Neil S.; Emmerson, Peter T.

doi:10.1099/0022-1317-74-12-2539

Cited by 288 publications

(213 citation statements)

References 40 publications

(38 reference statements)

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“…The P protein is encoded by the unedited mRNA; the addition of a single G residue to the editing site would yield a predicted V protein; and the addition of 2 G residues would yield a predicted W protein, as is the case with NDV (Steward et al, 1993). The putative editing site of the strain Yucaipa P gene is 5′-AAAAAGGGG (mRNA sense) at nt position 2090-2102 in the viral RNA genome, while the P gene editing sequence of NDV and other paramyxoviruses with similar coding strategy is AAAAA(A)GGG (Fig.…”

Section: The Phosphoprotein (P) Gene and P/v/w Editingmentioning

confidence: 99%

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

et al. 2008

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The complete RNA genome sequence of avian paramyxovirus (APMV) serotype 2, strain Yucaipa isolated from chicken has been determined. With genome size of 14,904 nucleotides (nt), strain Yucaipa is consistent with the "rule of six" and is the smallest virus reported to date among the members of subfamily Paramyxovirinae. The genome contains six non-overlapping genes in the order 3′-N-P/V-M-F-HN-L-5′. The genes are flanked on either side by highly-conserved transcription start and stop signals and have intergenic sequences varying in length from 3 to 23 nt. The genome contains a 55 nt leader sequence at 3′ end and a 154 nt trailer sequence at 5′ end. Alignment and phylogenetic analysis of the predicted amino acid sequences of strain Yucaipa proteins with the cognate proteins of viruses of all of the five genera of family Paramyxoviridae showed that APMV-2 strain Yucaipa is more closely related to APMV-6 than APMV-1.

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Section: The Phosphoprotein (P) Gene and P/v/w Editingmentioning

confidence: 99%

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

et al. 2008

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“…Replication of the viral genome is dependent upon encapsidation of the RNA by the nucleocapsid protein (NP) which acts as a template for a polymerase complex comprising the large, polymerase protein (L) and phosphoprotein (P) cofactor (Hamaguchi et al, 1983). In common with several members of the family Paramyxoviridae, NDV produces a further protein (V) by editing of the P gene at position 484 (Steward et al, 1993). The function of this protein is unknown, although a zinc-binding activity for the NDV V protein has been recently described .…”

mentioning

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Assembly of recombinant Newcastle disease virus nucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein.

Errington

Emmerson

1997

Journal of General Virology

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A recombinant baculovirus expressing the nucleocapsid gene (NP) of Newcastle disease virus (NDV), a member of the genus Rubulavirus, has been generated and shown to express the native protein to high levels in insect cells. In contrast to the NP protein of the rubulavirus human parainfluenza virus 2, the NDV protein has been demonstrated by electron microscopy and caesium chloride gradient analysis to be capable of self-assembly in vivo to form nucleocapsid-like structures in the absence of other NDV proteins. These structures, which contained RNA that was resistant to micrococcal nuclease digestion, were also observed when the protein was expressed in E. coli, a phenomenon which was not inhibited by the presence of a 40 amino acid fusion region at the amino terminus of the protein. Further, the formation of these structures was inhibited by the co-expression of the phosphoprotein (P). Therefore, we conclude that the P protein acts as a chaperone, preventing uncontrolled encapsidation of non-viral RNA by NP protein.Newcastle disease virus (NDV) is the aetiological agent for the devastating disease of poultry more commonly known as fowl pest. A member of the family Paramyxoviridae, NDV has recently been reclassified into the genus Rubulavirus (Murphy et al., 1995). Other members of this genus include mumps virus and simian virus 5 (SV5) in addition to the human parainfluenza viruses 2, 4a and 4b (hPIV2, 4a and 4b). The genome of NDV, which comprises a single strand of negative-sense RNA, encodes directly six structural genes in the order 3h-NP-P-M-

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“…The genome comprises six genes, which encode the nucleocapsid protein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), haemagglutinin-neuraminidase (HN) and large polymerase protein (L). In addition to these gene products, additional proteins (designated V and W protein) may be produced by an RNA-editing event that occurs during transcription of the P gene (Steward et al, 1993). NDV lacks the gene encoding the small hydrophobic (SH) protein, which is present in some members of the Rubulavirus genus.…”

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confidence: 99%

“…First, NDV edits its P gene mRNA from P to V instead of V to P (Steward et al, 1993) (Table 1). In accordance with this finding is the observation that the nucleotide sequence surrounding the mRNA editing site is similar in NDV LaSota and the paramyxoviruses and morbilliviruses (AAAAAGGG) but differs from that of the rubulaviruses (TTTAAGAGGGG).…”

mentioning

confidence: 99%

Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae.

Leeuw¹,

Peeters²

1999

Journal of General Virology

274

162

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We have completely sequenced the genome of Newcastle disease virus (NDV) vaccine strain LaSota. The sequences of the 3h-and 5h-terminal ends of the RNA genome were determined by sequencing cDNA fragments generated by rapid amplification of cDNA ends. The entire genomic sequence, which was established by sequencing cDNA fragments generated by high-fidelity RT-PCR, consists of 15 186 nt. Comparison of the 5h-terminal sequence of NDV LaSota with the 5h-terminal sequences of ten members of the Paramyxovirinae showed that NDV LaSota has an unusually long 5h untranscribed region. Comparison of the entire genomic sequences showed that NDV is only distantly related to the other members of the genus Rubulavirus, to which NDV has been assigned. In this paper we present data which suggest that NDV should not be classified in the genus Rubulavirus, but instead should be considered as a member of a new genus within the subfamily Paramyxovirinae.Newcastle disease is a serious disease of poultry that can cause severe economic losses in many countries. The causative agent of the disease is Newcastle disease virus (NDV), also called avian paramyxovirus type-1. NDV is a member of the genus Rubulavirus of the subfamily Paramyxovirinae (family Paramyxoviridae, order Mononegavirales) (Rima et al., 1995). NDV strains are classified on the basis of their pathogenicity for chickens into highly pathogenic (velogenic), intermediate (mesogenic) and apathogenic (lentogenic) strains (Hanson,

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RNA editing in Newcastle disease virus

Cited by 288 publications

References 40 publications

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

Assembly of recombinant Newcastle disease virus nucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein.

Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae.

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