Risk factors for Pseudomonas aeruginosa acquisition in intensive care units: a prospective multicentre study

Venier, A.G.; Leroyer, Christophe; Slekovec, C.; Talon, D.; Bertrand, Xavier; Parer, Sylvie; Alfandari, S.; Guerin, J. M.; Mégarbane, Bruno; Lawrence, Christine; Clair, Bernard; Lepape, Alain; Perraud, M.; Cassier, Pierre; Trivier, Dominique; Boyer, Alexandre; Dubois, Véronique; Asselineau, Julien; Rogues, A.-M.; Thiébaut, Rodolphe

doi:10.1016/j.jhin.2014.06.018

Cited by 79 publications

(67 citation statements)

References 41 publications

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“…This microorganism has become increasingly important due to associated prevalence, pathogenicity, and increasing resistance to antimicrobial treatments, causing morbidity and mortality. [1][2] Pseudomonas associated health care infections include respiratory tract infections, [3][4] urinary tract infections, 5 surgical and burn wound infections 6,7 and bloodstream infections. 8,9 According to Neonatal Infection Surveillance Network, between 2005 and 2011, in the United Kingdom, Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

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ABSTRACT:In recent years, atmospheric cold plasma (ACP) has been widely investigated for potential application as an alternative decontamination technology in biomedical and healthcare sectors. In this study, the antimicrobial efficacy of ACP against Pseudomonas aeruginosa biofilms was investigated. The 48-h biofilms were treated inside sealed polypropylene containers with a high-voltage dielectric barrier discharge (DBD) ACP (80 kV RMS ) and subsequently stored for 24 h at room temperature. Treatment for 60 s by either the direct or indirect mode of ACP exposure (inside or outside plasma discharge, respectively) reduced bacterial populations by an average of 5.4 log cycles from an initial 6.6 log 10 CFU/mL. Increasing the treatment time from 60 s to 120 s and 300 s reduced biofilms to undetectable levels. According to XTT assay (a metabolic activity assay), an extended treatment time of 300 s was necessary to reduce metabolic activity of cells in biofilms by an average of 70%. Further investigation of biofilm viability by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) demonstrated that extended ACP treatment had a detrimental effect on the viability of P. aeruginosa through disintegration of both bacterial cells and the biofilm matrix. The results of this study demonstrate the potential of a novel, in-package, high-voltage ACP decontamination approach for the inactivation of bacterial biofilms.

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Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

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show abstract

“…15 A multicentric prospective study recently established tap contamination in patient rooms as an important environmental risk factor for P. aeruginosa acquisition. 16 Several factors promote water contamination, including the type of faucet, 9,17,18 the presence and type of aerator on the faucet, 19 the volume of mixed hot and cold water, 17 the alignment of the sink drain, 20 construction or renovation settings, and ICU vs non-ICU settings. 3,20 Once contaminated, eradication of P. aeruginosa in the water system is challenging and often results in replacing related devices 5,[7][8][9][10][11][12]14 or installing point-of-use 0.2-μm filters.…”

mentioning

confidence: 99%

Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

Bédard

Laferrière

Charron

et al. 2015

Infect. Control Hosp. Epidemiol.

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OBJECTIVETo perform a post-outbreak prospective study of the Pseudomonas aeruginosa contamination at the faucets (water, aerator and drain) by culture and quantitative polymerase chain reaction (qPCR) and to assess environmental factors influencing occurrenceSETTINGA 450-bed pediatric university hospital in Montreal, CanadaMETHODSWater, aerator swab, and drain swab samples were collected from faucets and analyzed by culture and qPCR for the post-outbreak investigation. Water microbial and physicochemical parameters were measured, and a detailed characterization of the sink environmental and design parameters was performed.RESULTSThe outbreak genotyping investigation identified drains and aerators as the source of infection. The implementation of corrective measures was effective, but post-outbreak sampling using qPCR revealed 50% positivity for P. aeruginosa remaining in the water compared with 7% by culture. P. aeruginosa was recovered in the water, the aerator, and the drain in 21% of sinks. Drain alignment vs the faucet and water microbial quality were significant factors associated with water positivity, whereas P. aeruginosa load in the water was an average of 2 log higher for faucets with a positive aerator.CONCLUSIONSP. aeruginosa contamination in various components of sink environments was still detected several years after the resolution of an outbreak in a pediatric university hospital. Although contamination is often not detectable in water samples by culture, P. aeruginosa is present and can recover its culturability under favorable conditions. The importance of having clear maintenance protocols for water systems, including the drainage components, is highlighted.Infect. Control Hosp. Epidemiol. 2015;36(11):1283–1291

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“…34,35 Water systems can act as a source of S marcescens 36 and P aeruginosa. 19,35,37,38 Although we found P aeruginosa and Serratia spp to have the highest rate of BSI in exposed infants of all pathogens analyzed, they were also those with the lowest incidence density. The rate of BSI in exposed infants, defined by a BSI in another infant, does not reflect the general contagiousness of a pathogen but the risk for a cluster of BSI after an index case with the specific pathogen.…”

Section: Discussionmentioning

confidence: 56%

Pathogen-Specific Clustering of Nosocomial Blood Stream Infections in Very Preterm Infants

Reichert¹,

Piening

Geffers

et al. 2016

Pediatrics

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Risk factors for Pseudomonas aeruginosa acquisition in intensive care units: a prospective multicentre study

Cited by 79 publications

References 41 publications

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Post-Outbreak Investigation of Pseudomonas aeruginosa Faucet Contamination by Quantitative Polymerase Chain Reaction and Environmental Factors Affecting Positivity

Pathogen-Specific Clustering of Nosocomial Blood Stream Infections in Very Preterm Infants

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