Rickettsia rickettsii, Rickettsia felis and Rickettsia sp. TwKM03 infecting Rhipicephalus sanguineus and Ctenocephalides felis collected from dogs in a Brazilian spotted fever focus in the State of Rio De Janeiro/Brazil

Gehrke, Flávia S.; Souza, Eliana Rodrigues de; Ribeiro, Alberto F.; Marrelli, Mauro Toledo; Schumaker, Teresinha Tizu Sato

doi:10.1111/j.1469-0691.2008.02229.x

Cited by 36 publications

(32 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The proportion of positive pools (for the three genes) of cat fleas (54/132; 41%) is consistent with similar studies in different countries from all continents, confirming the worldwide distribution of this emergent pathogen. [2][3][4] However, the MIR reported here for C. felis (5.3%) is lower than MIRs reported in other countries, like Brazil (14.3%), 34 Taiwan (8.2%), 35 and the United States (13.3%). 33 We must emphasize that these rates correspond to MIRs, because they were calculated with the assumption that only one flea from each positive pool was positive for the Rickettsia gene analyzed; therefore, positive pools were not further corroborated to obtain the results for individual fleas, because we had limited resources.…”

Section: Discussioncontrasting

confidence: 47%

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Ramírez-Hernández

Montoya

Martínez

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Rickettsioses caused by Rickettsia felis are an emergent global threat. Historically, the northern region of the province of Caldas in Colombia has reported murine typhus cases, and recently, serological studies confirmed high seroprevalence for both R. felis and R. typhi. In the present study, fleas from seven municipalities were collected from dogs, cats, and mice. DNA was extracted and amplified by polymerase chain reaction (PCR) to identify gltA, ompB, and 17kD genes. Positive samples were sequenced to identify the species of Rickettsia. Of 1,341 fleas, Ctenocephalides felis was the most prevalent (76.7%). Positive PCR results in the three genes were evidenced in C. felis (minimum infection rates; 5.3%), C. canis (9.2%), and Pulex irritans (10.0%). Basic Local Alignment Search Tool (BLAST) analyses of sequences showed high identity values ( 98%) with R. felis, and all were highly related by phylogenetic analyses. This work shows the first detection of R. felis in fleas collected from animals in Colombia.

show abstract

Section: Discussioncontrasting

confidence: 47%

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Ramírez-Hernández

Montoya

Martínez

et al. 2013

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Although most reports on R. felis were associated to the flea Ctenocephalides felis felis collected in cats and/or dogs (LABRUNA et al, 2007b;NAVA et al, 2008;OLIVEIRA et al, 2008;GEHRKE et al, 2009), future studies should be conducted to fully establish flea rickettsial burden.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Fortes

Silveira

Moraes‐Filho

et al. 2010

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Seroprevalence of Rickettsia bellii and AbstractBrazilian spotted fever (BSF) is a vector-borne zoonosis caused by Rickettsia rickettsii bacteria. Dogs can be host sentinels for this bacterium. The aim of the study was to determine the presence of antibodies against Rickettsia spp. in dogs from the city of São José dos Pinhais, State of Paraná, Southern Brazil, where a human case of BSF was first reported in the state. Between February 2006 and July 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (IFA) against R. rickettsii and R. parkeri. All sera that reacted at least to one of Rickettsia species were tested against the six main Rickettsia species identified in Brazil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii and R. felis. Sixteen samples (4.4%) reacted to at least one Rickettsia species. Among positive animals, two dogs (15.5%) showed suggestive titers for R. bellii exposure. One sample had a homologous reaction to R. felis, a confirmed human pathogen. Although Rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to R. bellii and R. felis in Southern Brazil.Keywords: Brazilian spotted fever, Rickettsia rickettsii, sentinels, indirect immunofluorescence assay. ResumoA febre maculosa brasileira (FMB) é uma zoonose veiculada por carrapatos e causada pela bactéria Rickettsia rickettsii, podendo os cães ser hospedeiros sentinelas para essa bactéria. O objetivo do estudo foi determinar a presença de anticorpos contra Rickettsia spp. em cães de São José dos Pinhais, estado do Paraná, Sul do Brasil. Entre fevereiro de 2006 e julho de 2007, amostras séricas de 364 cães foram coletadas e testadas na diluição de 1:64 por Reação de Imunofluorescência Indireta (RIFI) contra R. rickettsii e R. parkeri. Todos os soros reagentes para pelo menos uma espécie de Rickettsia foram testados contra as seis principais espécies de Rickettsia identificadas no Brasil: R. rickettsii, R. parkeri, R. bellii, R. rhipicephali, R. amblyommii e R. felis. Dezesseis amostras (4,4%) reagiram para pelo menos uma espécie de Rickettsia. Dos animais positivos, dois cães (15,5%) apresentaram títulos sugestivos de exposição a R. bellii. Uma amostra apresentou reação homóloga frente à R. felis, um agente patogênico confirmado para seres humanos. Muito embora os resultados demonstrem uma baixa prevalência de Rickettsia spp. em cães, sugerindo um baixo risco de infecção humana, este estudo relatou pela primeira vez a evidência de exposição a R. bellii e R. felis em cães no Sul do Brasil.Palavras-chave: Febre maculosa brasileira, Rickettsia rickettsii, sentinelas, reação de imunofluorescência indireta.

show abstract

“…In addition, because A. aureolatum rarely attacks humans, the incidence of RMSF is also low in these specific areas of endemicity in southeastern Brazil (163). Finally, a number of recent studies from Brazil reported R. rickettsii infecting Rhipicephalus sanguineus ticks collected from dogs in areas where RMSF is endemic, where dogs are frequently infested by A. aureolatum or A. cajennense ticks (28,162,(164)(165)(166), and reports have also come from an area where RMSF is not endemic (167). As it has been shown that R. rickettsii is maintained by transstadial and transovarial transmission in Brazilian Rhipicephalus sanguineus ticks (26,28) and that the domestic dog is an efficient amplifier host of R. rickettsii in Rhipicephalus sanguineus ticks (26), studies are needed to elucidate the participation of this tick species in the epidemiology of RMSF in South America.…”

Section: South Americamentioning

confidence: 99%

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

et al. 2013

View full text Add to dashboard Cite

SUMMARY Tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus Rickettsia . These zoonoses are among the oldest known vector-borne diseases. However, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. Several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections, and novel Rickettsia species of undetermined pathogenicity continue to be detected in or isolated from ticks around the world. This remarkable expansion of information has been driven largely by the use of molecular techniques that have facilitated the identification of novel and previously recognized rickettsiae in ticks. New approaches, such as swabbing of eschars to obtain material to be tested by PCR, have emerged in recent years and have played a role in describing emerging tick-borne rickettsioses. Here, we present the current knowledge on tick-borne rickettsiae and rickettsioses using a geographic approach toward the epidemiology of these diseases.

show abstract

Rickettsia rickettsii, Rickettsia felis and Rickettsia sp. TwKM03 infecting Rhipicephalus sanguineus and Ctenocephalides felis collected from dogs in a Brazilian spotted fever focus in the State of Rio De Janeiro/Brazil

Abstract: Rickettsia rickettsii, Rickettsia felis and Rickettsia sp. TwKM03 infecting Rhipicephalus sanguineus and Ctenocephalides felis collected from dogs in a Brazilian spotted fever focus in the State of Rio De Janeiro ⁄ Brazil

Cited by 36 publications

References 5 publications

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, São José dos Pinhais, State of Paraná, Brazil

Update on Tick-Borne Rickettsioses around the World: a Geographic Approach

Contact Info

Product

Resources

About