Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Ramírez-Hernández, Alejandro; Montoya, Viviana; Martínez, Alejandra; Pérez, Jorge Enrique; Mercado, Marcela; Ossa, Alberto de la; Vélez, Carolina; Estrada, Gloria Inés; Correa, Maria I.; Duque, Laura; Ariza, Juan S.; Henao, Cesar; Valbuena, Gustavo; Hidalgo, Marylin

doi:10.4269/ajtmh.12-0698

Cited by 29 publications

(33 citation statements)

References 43 publications

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“…has not been demonstrated in fleas from Ecuador. However, R. felis has been found in South American fleas from Brazil, Peru, Uruguay, Chile, Argentina and Colombia [ 11 , 25 , 28 - 31 ]. In addition, human infection with R. felis in South America has been confirmed in Brazil by molecular methods [ 32 ], and human serological evidence of R. felis infection has been recently reported in Colombia [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

`Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

et al. 2014

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BackgroundFlea-borne infections are distributed worldwide. Up to date there are no reports about microorganisms associated to fleas in Ecuador.MethodsSeventy-one Pulex irritans and 8 Ctenocephalides felis fleas were removed from dogs in two Ecuadorian areas (Pastaza and Chimborazo Provinces) in December 2012. DNA extracts were tested by polymerase chain reaction (PCR) assays targeting universal 16S rRNA, as well as screened for the presence of Rickettsia spp. (gltA, htrA, ompB, sca4 and ompA genes) and Bartonella spp. (rpoB, gltA and ITS genes).ResultsOur results showed the presence of ‘Candidatus Rickettsia asemboensis’ (highly similar to R. felis) in C. felis and Wolbachia spp. endosimbionts in P. irritans collected from animals in Ecuador. No fleas were found to be positive for any Bartonella species or Yersinia pestis.ConclusionsClinicians should be aware of the potential risk of this new Candidatus Rickettsia sp. and keep in mind other flea-borne infections since these flea species frequently bite humans.

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Section: Resultsmentioning

confidence: 99%

`Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

et al. 2014

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“…that include species that cause anaplasmosis and ehrlichiosis, both intracellular bacteria that infect and destroy white blood cells, plus Staphylococcus spp. and Streptococcus spp [74][75][76]. For the majority of these pathogens, there are no previous reports of occurrence in snow leopards in the wild or in captive settings.…”

Section: Discussionmentioning

confidence: 99%

Health and zoonotic Infections of snow leopardsPanthera unicain the South Gobi desert of Mongolia

Esson

Skerratt

Berger

et al. 2019

Infection Ecology & Epidemiology

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Background : Snow leopards, Panthera uncia , are a threatened apex predator, scattered across the mountains of Central and South Asia. Disease threats to wild snow leopards have not been investigated. Methods and Results : Between 2008 and 2015, twenty snow leopards in the South Gobi desert of Mongolia were captured and immobilised for health screening and radio-collaring. Blood samples and external parasites were collected for pathogen analyses using enzyme-linked immunosorbent assay (ELISA), microscopic agglutination test (MAT), and next-generation sequencing (NGS) techniques. The animals showed no clinical signs of disease, however, serum antibodies to significant zoonotic pathogens were detected. These pathogens included, Coxiella burnetii , (25% prevalence), Leptospira spp., (20%), and Toxoplasma gondii (20%). Ticks collected from snow leopards contained potentially zoonotic bacteria from the genera Bacillus, Bacteroides, Campylobacter, Coxiella, Rickettsia, Staphylococcus and Streptococcus . Conclusions : The zoonotic pathogens identified in this study, in the short-term did not appear to cause illness in the snow leopards, but have caused illness in other wild felids. Therefore, surveillance for pathogens should be implemented to monitor for potential longer- term disease impacts on this snow leopard population.

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“…They were processed for DNA extraction using a modified protocol of the commercial kit DNeasy Blood and Tissue (QIAGEN Inc., Valencia, CA) with the addition of guanidine thiocyanate (DNAzol; Invitrogen ™ , Life Technologies Corp., Grand Island, NY). 15 DNA extracts were used for molecular detection of Rickettsia spp. by conventional and nested polymerase chain reaction (PCR) assays, ensuring 150 ng/μL of DNA per reaction.…”

Section: Methodsmentioning

confidence: 99%

Epidemiology of Spotted Fever Group Rickettsioses and Acute Undifferentiated Febrile Illness in Villeta, Colombia

Faccini-Martínez

Ramírez-Hernández

Barreto

et al. 2017

The American Journal of Tropical Medicine and Hygiene

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Etiology of acute undifferentiated febrile syndrome (AUFS) is often unknown, leading to inaccurate diagnosis and treatment. Villeta town has been identified as an endemic area for spotted fever group (SFG) rickettsioses but little is known about possible amplifier hosts and other species different from. Besides, few studies have approached other AUFS etiologies in the region. We investigated the role of dengue, leptospirosis, rickettsioses, human anaplasmosis, and Q fever as possible causes of AUFS in patients from Villeta. Sera specimens and ticks from animals as well as ticks from vegetation were studied for the presence of different spp. Among 104 sera from patients with AUFS, 16.4%, 24.0%, and 2.9% patients seroconverted to dengue,, and SFG , respectively, with a case of probable coinfection or cross-reaction with. None of the samples were reactive for . Sera samples from 74 horses, 118 dogs, and 62 bovines were collected and showed 33.8%, 14.4%, and 50.0% of seroprevalence for SFG, respectively. A total of 1,287 ixodid ticks were collected from animals/vegetation and processed in pools for polymerase chain reaction. Among them, 1.7% was positive for genes, and, , and spp. were found. These results confirm the circulation of dengue, different SFG species and the relevance of other etiologies like leptospirosis and human anaplasmosis. Further studies must identify different epidemiological variables to establish proper surveillance and control programs.

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Molecular Detection of Rickettsia felis in Different Flea Species from Caldas, Colombia

Cited by 29 publications

References 43 publications

`Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

`Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador

Health and zoonotic Infections of snow leopardsPanthera unicain the South Gobi desert of Mongolia

Epidemiology of Spotted Fever Group Rickettsioses and Acute Undifferentiated Febrile Illness in Villeta, Colombia

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