Rice MutLγ, the MLH1–MLH3 heterodimer, participates in the formation of type I crossovers and regulation of embryo sac fertility

Mao, Bigang; Zheng, Wenjie; Huang, Zhen; Peng, Yan; Shao, Ye; Liu, Citao; Tang, Li; Hu, Yuanyi; Li, Yaokui; Hu, Liming; Zhang, Dan; Yuan, Zhicheng; Yuan, Longping; Liu, Yao-Guang; Zhao, Baisuo

doi:10.1111/pbi.13563

Cited by 12 publications

(9 citation statements)

References 77 publications

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“…In rice, MutLγ, the MLH1–MLH3 heterodimer, plays a major role in regulating type I crossover formation during meiosis. OsMLH1 and OsMLH3 preferentially accumulate in the inflorescence and their knockout mutants exhibited normal plant morphology during the vegetative stage, but a significantly reduced seed‐setting rate at the reproductive stage (Mao et al, 2021; Xin et al, 2021). As overexpression of MLH1dn partially, rather than completely, inhibits MLH1 functions, the effects of MHL1dn on maize fertility should be less severe than MHL1 knockout and could be eliminated in the next generation by segregation.…”

Section: Resultsmentioning

confidence: 99%

Optimized prime editing efficiently generates heritable mutations in maize

Qiao

Wang

et al. 2023

JIPB

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Low efficiency is the main obstacle to using prime editing in maize (Zea mays). Recently, prime-editing efficiency was greatly improved in mammalian cells and rice (Oryza sativa) plants by engineering primeediting guide RNAs (pegRNAs), optimizing the prime editor (PE) protein, and manipulating cellular determinants of prime editing. In this study, we tested PEs optimized via these three strategies in maize. We demonstrated that the ePE5max system, composed of PEmax, epegRNAs (pegRNA-evopreQ. 1), nicking single guide RNAs (sgRNAs), and MLH1dn, efficiently generated heritable mutations that conferred resistance to herbicides that inhibit 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), acetolactate synthase (ALS), or acetyl CoA carboxylase (ACCase) activity. Collectively, we demonstrate that the ePE5max system has sufficient efficiency to generate heritable (homozygous or heterozygous) mutations in maize target genes and that the main obstacle to using PEs in maize has thus been removed.

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Section: Resultsmentioning

confidence: 99%

Optimized prime editing efficiently generates heritable mutations in maize

Qiao

Wang

et al. 2023

JIPB

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“…LLPS is reported to mediate the localization of proteins to the pre-existing phase separated, accelerate the biological reactions, and perform other functions (Alberti et al, 2019;Buttress et al, 2022). LLPS also exists in the nucleus: when co-expressed in live leaf imaging, the aggregation of GBPL3 in the nucleus can exclude CDK8 (Huang et al, 2021). LLPS at specific genomic sites can promote targeted condensation and bring distal genomic loci together (Shin et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

A point mutation in the meiotic crossover formation gene HEI10/TFS2 leads to thermosensitive genic sterility in rice

Zhang,

Guo,

Wang

et al. 2024

The Plant Journal

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SUMMARYThermosensitive genic female sterility (TGFS) is a promising property to be utilized for hybrid breeding. Here, we identified a rice TGFS line, tfs2, through an ethyl methyl sulfone (EMS) mutagenesis strategy. This line showed sterility under high temperature and became fertile under low temperature. Few seeds were produced when the tfs2 stigma was pollinated, indicating that tfs2 is female sterile. Gene cloning and genetic complementation showed that a point mutation from leucine to phenylalanine in HEI10 (HEI10tfs2), a crossover formation protein, caused the TGFS trait of tfs2. Under high temperature, abnormal univalents were formed, and the chromosomes were unequally segregated during meiosis, similar to the reported meiotic defects in oshei10. Under low temperature, the number of univalents was largely reduced, and the chromosomes segregated equally, suggesting that crossover formation was restored in tfs2. Yeast two‐hybrid assays showed that HEI10 interacted with two putative protein degradation‐related proteins, RPT4 and SRFP1. Through transient expression in tobacco leaves, HEI10 were found to spontaneously aggregate into dot‐like foci in the nucleus under high temperature, but HEI10tfs2 failed to aggregate. In contrast, low temperature promoted HEI10tfs2 aggregation. This result suggests that protein aggregation at the crossover position contributes to the fertility restoration of tfs2 under low temperature. In addition, RPT4 and SRFP1 also aggregated into dot‐like foci, and these aggregations depend on the presence of HEI10. These findings reveal a novel mechanism of fertility restoration and facilitate further understanding of HEI10 in meiotic crossover formation.

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“…The FM-line system is superior to the traditional HRS production method and other biotechnology-based systems in several aspects. First, relative to the other FS mutants, such as ptb1 (Li S.C. et al, 2013) and fsv1 (Liu et al, 2019;Mao et al, 2021), here, the FS lines were created by hybridization breeding and using a natural mutant fst (Lee et al, 2013a), and the plants propagated were completely sterile in different genetic backgrounds and under different environmental conditions. Second, compared with the current 2-and 3-line systems of HRS technology, the FM-line system facilitated the mechanization of high-quality and efficient HRS production using a mixed-planting and mixed-harvesting approach for FS and MS lines.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, only a few FS mutant genes, such as the fst (Lee et al, 2002(Lee et al, , 2013a, ptb1 (Li S.C. et al, 2013;Xia et al, 2019), and fsv1 (Liu et al, 2019;Mao et al, 2021), have been identified in rice. However, for the commercial production of hybrids, FS acting as the pollen donor parent is only feasible if the FS line is completely sterile.…”

Section: Introductionmentioning

confidence: 99%

Construction of a Novel Female Sterility System for Hybrid Rice

Wei

Guo

et al. 2022

Front. Plant Sci.

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The main constraints of current hybrid rice technology using male sterility (MS) are the low yield and high labor costs of hybrid rice seed (HRS) production. Therefore, there is an urgent need for innovative new hybrid rice technology. Fortunately, we discovered a unique spontaneous sporophytic female-sterile rice mutant controlled by a single recessive locus in the nucleus. Because female-sterile mutant lines cannot produce any selfed-seeds but their pollen has totally normal functions, female sterility (FS) lines may be considered ideal pollen donors to replace the female-fertile pollen donor parent lines currently used in the HRS production. In this study, a genetically engineered FS-based system was constructed to propagate a pure transgene-free FS line using a bentazon herbicide screening. Additionally, the ability of the FS + MS (FM)-line system, with mixed plantings of FS and MS lines, to produce HRS was tested. The pilot field experiment results showed that HRS of the FM-line system was more efficient compared with the conventional FS to MS strip planting control mode. Thus, this study provides new insights into genetic engineering technology and a promising strategy for the utilization of FS in hybrid rice.

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Rice MutLγ, the MLH1–MLH3 heterodimer, participates in the formation of type I crossovers and regulation of embryo sac fertility

Cited by 12 publications

References 77 publications

Optimized prime editing efficiently generates heritable mutations in maize

Optimized prime editing efficiently generates heritable mutations in maize

A point mutation in the meiotic crossover formation gene HEI10/TFS2 leads to thermosensitive genic sterility in rice

Construction of a Novel Female Sterility System for Hybrid Rice

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