Construction of a Novel Female Sterility System for Hybrid Rice

Wei, Li; Guo, Xiaoqiong; Wu, Wenbin; Yu, Weilin; Li, Shichuan; Luo, Di; Wang, Tianjie; Zhu, Qian; Chen, Lijuan; Lee, Dongsun

doi:10.3389/fpls.2021.815401

Cited by 3 publications

(8 citation statements)

References 37 publications

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“…However, the ptb1 mutant is not completely sterile and has a seed-setting rate of 1.8% (Li et al 2013 ); this will cause 0.6–1.2% of female infertile seed contamination of the hybrid seed during commercial production (Xia et al 2019 ). Li et al ( 2022 ) used a different strategy by constructing a transgene cassette consisting of the wild-type female fertility gene FST and the BTZ-RNAi expression module, which was then transformed into the female sterile mutant fst . The function of BTZ-RNAi is to inhibit the expression of CYP81A6 and make the transgenic plant sensitive to herbicide bentazon.…”

Section: Discussionmentioning

confidence: 99%

“…Self-pollination of the hemizygous transgenic T 0 plants produced 3:1 transgenic and nontransgenic progeny. The transgenic plants were eliminated by bentazon spray, and the transgene-free female sterile line stayed alive (Li et al 2022 ). The advantage of this system is that the fst mutant is completely female sterile (Li et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…The transgenic plants were eliminated by bentazon spray, and the transgene-free female sterile line stayed alive (Li et al 2022 ). The advantage of this system is that the fst mutant is completely female sterile (Li et al 2022 ). However, it has a problem in continuing propagation of the maintainer line.…”

Section: Discussionmentioning

confidence: 99%

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Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene

Wang,

Yuan,

Wang

et al. 2024

Rice

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Background Hybrid rice has significant yield advantage and stress tolerance compared with inbred rice. However, production of hybrid rice seeds requires extensive manual labors. Currently, hybrid rice seeds are produced by crosspollination of male sterile lines by fertile paternal lines. Because seeds from paternal lines can contaminate the hybrid seeds, mechanized production by mixed-seeding and mixed-harvesting is difficult. This problem can be solved if the paternal line is female sterile. Results Here we identified a female infertile mutant named h569 carrying a novel mutation (A1106G) in the MEL2 gene that was previously reported to regulate meiosis entry both in male and female organs. h569 mutant is female infertile but male normal, suggesting that MEL2 regulates meiosis entry in male and female organs through distinct pathways. The MEL2 gene and h569 mutant gave us tools to construct female sterility maintaining systems that can be used for propagation of female sterile lines. We connected the wild-type MEL2 gene with pollen-killer gene ZmAA1 and seed-marker gene DsRed2 in one T-DNA cassette and transformed it into ZZH1607, a widely used restorer line. Transgenic line carrying a single transgene inserted in an intergenic region was selected to cross with h569 mutant. F2 progeny carrying homozygous A1106G mutation and hemizygous transgene displayed 1:1 segregation of fertile and infertile pollen grains and 1:1 segregation of fluorescent and non-fluorescent seeds upon self-fertilization. All of the non-fluorescent seeds generated female infertile plants, while the fluorescent seeds generated fertile plants that reproduced in the way as their previous generation. Conclusions These results indicated that the female sterility maintaining system constructed in the study can be used to breed and propagate paternal lines that are female infertile. The application of this system will enable mechanized production of hybrid rice seed by using the mixed-seeding and mixed harvesting approach, which will significantly reduce the cost in hybrid rice seed production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene

Wang,

Yuan,

Wang

et al. 2024

Rice

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“…The MADS-box gene FEMALE-STERILE (FST) is genetically sporophytic and plays a key role in regulating chalaza formation, integument morphogenesis, and the early development of the zygotic embryo and endosperm (Table 1 ) (Yin and Xue 2012 ; Lee et al 2013 ; Li et al 2022 ). The fst mutation confers complete female sterility in various genetic backgrounds under different environmental conditions without affecting vegetative growth or pollen viability.…”

Section: Construction Of Male-sterile Line and Maintainer Line In One...mentioning

confidence: 99%

Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review

Chen,

Wu,

Deng

et al. 2023

Rice

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Rice (Oryza sativa L.) is one of the most important food crops worldwide. The utilisation of heterosis (hybrid vigour) has played a significant role in increasing rice yield and ensuring food supply. Over the past 50 years, the first-generation three-line system based on cytoplasmic male sterility, and the second-generation two-line system based on environment-sensitive genic male sterility (EGMS), have been widely applied in hybrid rice production. However, the three-line system is restricted by the matching relationship among the three parental lines and allows only ~ 2–5% of germplasms to be explored for elite combinations. The environmental sensitivity of EGMS lines has posed serious risks to the production of hybrid seeds. These factors have hindered the development and applications of hybrid rice. Third-generation hybrid rice technology (TGHRT) is based on environment-insensitive genic male sterility, which can effectively overcome the intrinsic problems of the three-line and two-line systems. Since the establishment of TGHRT, numerous findings and innovations have been reported. This paper gives a brief review of traditional hybrid rice technologies and discusses the establishment of TGHRT, technical innovations in TGHRT, and future research that is necessary to promote the wide application of TGHRT in rice production.

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“…During the process of hybrid seed breeding in fields, restorer lines must be planted separately from male sterile lines and eliminated before harvesting to avoid contamination (Kim et al, 2007). In contrast, the TGFS lines would not need to be eliminated ahead of time in fields when harvesting (Li et al, 2021(Li et al, , 2022. Thus, the TGFS lines are expected to be valuable in hybrid seed breeding.…”

Section: Introductionmentioning

confidence: 99%

A point mutation in the meiotic crossover formation gene HEI10/TFS2 leads to thermosensitive genic sterility in rice

Zhang,

Guo,

Wang

et al. 2024

The Plant Journal

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SUMMARYThermosensitive genic female sterility (TGFS) is a promising property to be utilized for hybrid breeding. Here, we identified a rice TGFS line, tfs2, through an ethyl methyl sulfone (EMS) mutagenesis strategy. This line showed sterility under high temperature and became fertile under low temperature. Few seeds were produced when the tfs2 stigma was pollinated, indicating that tfs2 is female sterile. Gene cloning and genetic complementation showed that a point mutation from leucine to phenylalanine in HEI10 (HEI10tfs2), a crossover formation protein, caused the TGFS trait of tfs2. Under high temperature, abnormal univalents were formed, and the chromosomes were unequally segregated during meiosis, similar to the reported meiotic defects in oshei10. Under low temperature, the number of univalents was largely reduced, and the chromosomes segregated equally, suggesting that crossover formation was restored in tfs2. Yeast two‐hybrid assays showed that HEI10 interacted with two putative protein degradation‐related proteins, RPT4 and SRFP1. Through transient expression in tobacco leaves, HEI10 were found to spontaneously aggregate into dot‐like foci in the nucleus under high temperature, but HEI10tfs2 failed to aggregate. In contrast, low temperature promoted HEI10tfs2 aggregation. This result suggests that protein aggregation at the crossover position contributes to the fertility restoration of tfs2 under low temperature. In addition, RPT4 and SRFP1 also aggregated into dot‐like foci, and these aggregations depend on the presence of HEI10. These findings reveal a novel mechanism of fertility restoration and facilitate further understanding of HEI10 in meiotic crossover formation.

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Construction of a Novel Female Sterility System for Hybrid Rice

Cited by 3 publications

References 37 publications

Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene

Construction of a Female Sterility Maintaining System Based on a Novel Mutation of the MEL2 Gene

Establishment and Advances of Third-Generation Hybrid Rice Technology: A Review

A point mutation in the meiotic crossover formation gene HEI10/TFS2 leads to thermosensitive genic sterility in rice

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