Rht8 gene as an alternate dwarfing gene in elite Indian spring wheat cultivars

Grover, Gomti; Sharma, Achla; Gill, Harsimardeep S.; Srivastava, Puja; Bains, N. S.

doi:10.1371/journal.pone.0199330

Cited by 33 publications

(31 citation statements)

References 22 publications

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“…Высота пшеницы -сложный признак, который определяется различными группами генов и связан с морфологией и фертильностью колоса, временем цветения, продуктивностью растения и качеством зерна (Chen et al, 2016;Würschum et al, 2018a, b). Одним из путей повышения урожайности пшеницы, особенно в условиях интенсивного земледелия, является оптимизация высоты растений за счет создания низкостебельных сортов (Лукьяненко, 1970;Беспалова, 2001;Divashuk et al, 2013;Grover et al, 2018). Наибольшее значение в создании форм пшеницы с оптимальной высотой растений в настоящее время имеют аллельные варианты генов, определяющих высоту растения, обладающие фенотипическим эффектом снижения высоты растений.…”

Section: Introductionunclassified

Phenotypic effects of the dwarfing gene Rht-17 in spring durum wheat under two climatic conditions

et al. 2019

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Alleles of the genes, conferring a dwarfing phenotype, play a crucial role in wheat breeding, as they not only reduce plant height, ensuring their resistance to lodging, but also have a number of positive and negative pleiotropic effects on plant productivity. Durum wheat carries only two subgenomes (A and B), which limits the use of the D-subgenome genes and requires the expansion of the arsenal of dwarfing alleles and the study of their effects on height and agronomically important traits. We studied the effect of the gibberellin-insensitive allele Rht-B1p in the B2F2:3 families, developed by crossing Chris Mutant /#517//LD222 in a field experiment in Moscow and Krasnodar. In our experiments, plants homozygous for Rht-B1p were shorter than those homozygous for the wild-type allele Rht-B1a by 36.3 cm (40 %) in Moscow and 49.5 cm (48 %) in Krasnodar. In the field experiment in Krasnodar, each plant with Rht-B1p had one less internode than any plant with Rht-B1a, which additionally contributed to the decrease in plant height. Grain weight per main spike was lower in plants with Rht-B1p than in plants with Rht-B1a by 12 % in Moscow and by 23 % in Krasnodar due to a decrease in 1000 grain weight in both regions of the field experiment. The number of grains per main spike in plants with Rht-B1p was higher in comparison to that with Rht-B1a by 6.5 % in Moscow due to an increase in spikelet number per main spike and by 11 % in Krasnodar due to an increase in grain number per spikelet. The onset of heading in plants with Rht-B1p in comparison with the plants with the wild-type allele Rht-B1a was 7 days later in Krasnodar. The possibility and prospects for the use of Rht-B1p in the breeding of durum wheat are discussed.

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Section: Introductionunclassified

Phenotypic effects of the dwarfing gene Rht-17 in spring durum wheat under two climatic conditions

et al. 2019

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“…It reduces straw length by ca 10 cm and is widespread among the Yugoslavian cultivars of common wheat . Grover et al, (2018) reported a 13.3% reduction in plant height due to Rht8 gene as compared to lines lacking this gene. Presence of allele that provides the product of 192-bp was related to the decrease in plant height by about 7-8 cm (Kowalczyk, 2006).…”

Section: Discussionmentioning

confidence: 98%

Identification of allelic variants of Xgwm261 locus for Rht8 dwarfing gene in Pakistani wheat genetic resource

Tabasum¹,

Iqbal²,

Hameed³

2019

PAK.J.BOT.

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Semi dwarfing wheat gene (Rht8) is among the important Green Revolution genes to increase productivity by modifying plant stature. Plant height is among one of the important constituent of plant architecture that determines the stability, harvest index and yield of crop plants. Strong linkage of Xgwm261, a wheat microsatellite marker with the dwarfing gene (Rht8) resulting in reduced height makes it well known marker among the breeders. Using this functional SSR marker, Pakistani wheat varieties and land races were analyzed to access the allelic distribution/ variation at microsatellite Xgwm261 locus. The 165-bp and 174-bp alleles of Xgwm261 were most frequent (38 and 24% respectively) among studied genotypes. A 192-bp diagnostic allele of Rht8 gene was not detected in tested germplasm. Here we also report novel WMS261 variants of 182-bp, 188-bp, 196-bp and over 200-bp (205 and 215-bp) in some tested genotypes. For this microsatellite Xgwm261 locus the value of polymorphic information content (PIC) was 0.55. Conclusively, a high level of diversity for this locus was observed. Seven different microsatellite alleles were detected and a strong linkage of these variants at the Rht8 locus if identified will provide a better understanding for gene to environment interaction for designing appropriate marker assisted selection (MAS) for high yielder wheat cultivars. Observed higher frequency of major allele (165-bp) could possibly be due to usage of CIMMYT wheat materials in Pakistani breeding programs. Selection of appropriate alleles will be useful in the varietal improvements breeding programs.

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“…This gene is essential in the Gibberellin (GA) catabolic pathway and regulates plant growth by inactivating endogenous bioactive GAs. Previous studies indicated that many semi-dwarf genes had a negative effect on coleoptile length [3,10]. This gene may regulate the plant growth by GA pathway in multiple aspects, including the coleoptile elongation.…”

Section: Discussionmentioning

confidence: 99%

“…Longer coleoptiles can not only protect the crops from early drought but also improve water use efficiency, and are more sustainable in low water supply areas. In the past decades, coleoptile elongation has been studied mainly in wheat, with a main focus on dwarf or semidwarf genes that had only little effect on the coleoptile length [10]. Only a few other studies announced coleoptile length-related loci other than dwarf genes [3].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association mapping reveals novel genes associated with coleoptile length in a worldwide collection of barley

Luo

Hill

Zhou

et al. 2020

Preprint

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BackgroundDrought is projected to become more frequent and severe in a changing climate, which requires deep sowing of crop seeds to reach soil moisture. Coleoptile length is a key agronomic trait in cereal crops such as barley, as long coleoptiles are linked to drought tolerance and improved seedling establishment under early water-limited growing conditions. ResultsIn this study, we detected large genetic variation in a panel of 328 diverse barley (Hordeum vulgare L.) accessions. To understand the overall genetic basis of barley coleoptile length, all accessions were geminated in the dark and phenotyped for coleoptile length after 2 weeks. The investigated barleys had significant variation for coleoptile length. We then conducted genome-wide association studies (GWASs) with more than 30,000 molecular markers and identified 8 genes and 12 intergenic loci significantly associated with coleoptile length in our barley panel. The Squamosa promoter-binding-like protein 3 gene (SPL3) on chromosome 6H was identified as a major candidate gene. The missense variant on the second exon changed serine to alanine in the conserved SBP domain, which likely impacted its DNA-binding activity. ConclusionThis study provides genetic loci for seedling coleoptile length along with candidate genes for future potential incorporation in breeding programmes to enhance early vigour and yield potential in water-limited environments.

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Rht8 gene as an alternate dwarfing gene in elite Indian spring wheat cultivars

Cited by 33 publications

References 22 publications

Phenotypic effects of the dwarfing gene Rht-17 in spring durum wheat under two climatic conditions

Phenotypic effects of the dwarfing gene Rht-17 in spring durum wheat under two climatic conditions

Identification of allelic variants of Xgwm261 locus for Rht8 dwarfing gene in Pakistani wheat genetic resource

Genome-wide association mapping reveals novel genes associated with coleoptile length in a worldwide collection of barley

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