Identification of allelic variants of Xgwm261 locus for Rht8 dwarfing gene in Pakistani wheat genetic resource

Abstract

Semi dwarfing wheat gene (Rht8) is among the important Green Revolution genes to increase productivity by modifying plant stature. Plant height is among one of the important constituent of plant architecture that determines the stability, harvest index and yield of crop plants. Strong linkage of Xgwm261, a wheat microsatellite marker with the dwarfing gene (Rht8) resulting in reduced height makes it well known marker among the breeders. Using this functional SSR marker, Pakistani wheat varieties and land races were analyzed to access the allelic distribution/ variation at microsatellite Xgwm261 locus. The 165-bp and 174-bp alleles of Xgwm261 were most frequent (38 and 24% respectively) among studied genotypes. A 192-bp diagnostic allele of Rht8 gene was not detected in tested germplasm. Here we also report novel WMS261 variants of 182-bp, 188-bp, 196-bp and over 200-bp (205 and 215-bp) in some tested genotypes. For this microsatellite Xgwm261 locus the value of polymorphic information content (PIC) was 0.55. Conclusively, a high level of diversity for this locus was observed. Seven different microsatellite alleles were detected and a strong linkage of these variants at the Rht8 locus if identified will provide a better understanding for gene to environment interaction for designing appropriate marker assisted selection (MAS) for high yielder wheat cultivars. Observed higher frequency of major allele (165-bp) could possibly be due to usage of CIMMYT wheat materials in Pakistani breeding programs. Selection of appropriate alleles will be useful in the varietal improvements breeding programs.

EVALUTION OF THE EFFECT OF THE NEW FRACTION PAIR 1Dx1.5+1Dy10 INHERITED FROM Glu-Dt LOCUS OF Ae. Tauschii (DtDt, 2n=14) ON SOME QUALITATIVE INDICES IN THE PROGENIES OF SYNTHETIC WHEAT VARIETIES (BBАuАuDtDt, 2n=42)

“…The results from the investigations show that the researches on the genetics of the qualitative indices are important for the improvement of the breeding process since these results allow determining the relationships between the composition of the storage endosperm proteins and the technological properties of grain. (Tabasum et al, 2011;Carter et al, 2012).…”

Characterization of gamma ray induced mutations in PepYLCV-infecting local pepper in Karo Regency, Sumatra Utara, Indonesia

“…These results indicated that higher irradiation led to lower plant height, stem diameter, branch and leaf numbers. The research of [50] and [51] approved that each individual had different genotype response to gamma irradiation.…”