Rhodococcus maanshanensis sp. nov., a novel actinomycete from soil.

Rhodococcus defluvii sp. nov., isolated from wastewater of a bioreactor and formal proposal to reclassify [Corynebacterium hoagii] and Rhodococcus equi as Rhodococcus hoagii comb. nov.

“…nov. with strain Ca11 T as a representative type strain. The phylogenetic analysis based on nearly full-length 16S rRNA gene sequence analysis indicated that the R. equi 16S rRNA gene clade, including R. equi, R. kunmingensis, and strain Ca11 T represents a separate phylogenetic lineage, but assignment to the genus Rhodococcus remained, because no distinct chemotaxonomic differences were found to justify a reclassification and the proposal of a (Helmke & Weyland, 1984;Zhang et al, 2002Zhang et al, , 2005Klatte et al, 1994;Briglia et al, 1996;Yoon et al, 2000;Goodfellow et al, 2002;Takeuchi et al, 2002;Matsuyama et al, 2003;Li et al, 2004). +, Positive; W, weakly positive; 2, negative.…”

Bacillus oceanisediminis sp. nov., isolated from marine sediment

“…In particular, the isolate could be differentiated from Bacillus firmus ATCC 14575 T in that it was positive for utilization of D-galactose, inulin, myo-inositol, lactose, D-sorbitol and xylitol. Additional phenotypic properties are presented in the species description and Table 1.Biomass for most of the chemotaxonomic and molecular systematic studies was prepared as described by Zhang et al (2002), with the modification of using shake flasks of LB broth. Preparation of the cell wall and determination of the peptidoglycan composition were performed using the methods described by Schleifer (1985), with the modification that cellulose sheets instead of paper were used for TLC.…”

“…Biomass for most of the chemotaxonomic and molecular systematic studies was prepared as described by Zhang et al (2002), with the modification of using shake flasks of LB broth. Preparation of the cell wall and determination of the peptidoglycan composition were performed using the methods described by Schleifer (1985), with the modification that cellulose sheets instead of paper were used for TLC.…”

Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga

“…A glucose-yeast extract-malt extract (GYM) agar plate (comprising, l 21 : 4 g D-glucose, 4 g yeast extract, 10 g malt extract, 15 g agar; pH 7.3) was inoculated with a suspension of the soil and incubated at 37 u C for 7 days. The isolate that was obtained, strain 25B T , was maintained on LB agar (l 21 : 10 g tryptone, 5 g yeast extract, 10 g NaCl, 15 g agar; pH 7.0) at 4 u C and as glycerol suspensions (20 %, v/v) at 220 u C. Biomass for the chemotaxonomic and molecular systematic studies was prepared as described previously (Zhang et al, 2002) with the modification that the strain was grown in shake flasks of LB broth.Micromorphological and colonial properties of strain 25B T were examined on LB agar, Rourf's agar (Mulder & Deinema, 1992) and GYM agar after incubation at 37 u C for 3-7 days. Phase-contrast and electron microscopy of cells were performed as described by Andrews & Patel (1996).…”