Bacillus oceanisediminis sp. nov., isolated from marine sediment

Abstract

A Gram-stain-positive, spore-forming, rod-shaped and aerobic bacterium was isolated from a sediment sample from the South Sea in China. The isolate, designated H2 T , grew at 4-45 6C (optimum 37 6C) and pH 6-10 (optimum pH 7.0). The cell-wall peptidoglycan contained mesodiaminopimelic acid. The major isoprenoid quinone was MK-7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. The major fatty acid was iso-C 15 : 0 . The genomic DNA G+C content of strain H2 T was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a monophyletic clade with Bacillus firmus IAM 12464 T . DNA-DNA relatedness between the isolate and B. firmus ATCC 14575 T was low (27.5 %). Strain H2 T also had a phenotypic profile that readily distinguished it from its closest phylogenetic neighbours. It is evident from the combination of genotypic and phenotypic data that the organism should be classified in a novel species of the genus Bacillus, for which the name Bacillus oceanisediminis sp. nov. is proposed. The type strain is H2 T (5CGMCC 1.10115 T 5JCM 16506 T ).The application of chemotaxonomic, numerical phenetic and molecular systematic methods has led to an improved description and a division of the genus Bacillus. Some species have been reclassified in novel genera or transferred to other genera (Ash et al., 1993;Stackebrandt & Liesack, 1993;Shida et al., 1996;Wainø et al., 1999;Nazina et al., 2001;Yoon et al., 2001;Albert et al., 2007;Zhou et al., 2009). Many of these reclassifications are well supported by relatively conserved phenotypic traits such as the quinone system, peptidoglycan composition and polar lipid and cellular fatty acid profiles (Albert et al., 2007). Falsibacillus is a newly described genus, which currently comprises a single species, Falsibacillus pallidus comb. nov., and is represented by the type strain, CW 7 T . The taxon F. pallidus comb. nov. is linked to the illegitimately named taxon Bacillus pallidus Zhou et al. 2008 and the creation of the genus Falsibacillus was primarily based on significant chemotaxonomic differences in comparison with the type species of the genus Bacillus, Bacillus subtilis (Zhou et al., , 2009). The genus Bacillus currently encompasses more than 150 species with validly published names and contains at least six phylogenetically distinct groups on the basis of molecular analyses of 16S rRNA gene sequences (Ash et al., 1991;Nielsen et al., 1994;Schlesner et al., 2001;Yoon et al., 2004;Carrasco et al., 2007). Members of the genus Bacillus have been isolated from a wide variety of aquatic and terrestrial environments. A Gram-stain-positive bacterium, strain H2 T , was isolated during an ecological survey of marine organisms in China and found to have morphological properties consistent with the genus Bacillus.Strain H2 T was isolated from a marine sediment collected at a depth of 823 m in the South Sea, China. A standard dilution-plating technique was used to ...

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

“…The majority of the genera and species recovered in this study were from marsh sediments and have previously been described in saline environments except B. safensis, Bacillus thioparans and B. simplex (Ventosa et al, 1998;Okamoto et al, 2001;Yoon et al, 2001;2003;Chookietwattana, 2003;Sánchez-Porro et al, 2003;Borsodi et al, 2005;Fan et al, 2006;Pérez-Ibarra et al, 2007;Ventosa et al, 2008;Parvathi et al, 2009;Sasaki et al, 2009;Zhang et al, 2010;Bassey and Grigson, 2011). This finding extends our knowledge of the bacterial diversity in saline environments and indicates that the groups obtained, including potential new species, are well adapted.…”

Draft Genome Sequence of Bacillus oceanisediminis 2691

“…T was first isolated from sediment of the South Sea in China (11) and was found to be most closely related to Bacillus firmus (6) and Bacillus infantis (4) by 16S rRNA gene analysis. Recently, the Gram-negative Paenibacillus oceanisediminis sp.…”

Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant

“…The polar lipid analysis showed the predominance of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol (Fig. 2), which are characteristic of the genus Bacillus (Kämpfer et al, 2006;Vaishampayan et al, 2010;Zhang et al, 2010). The unidentified phospholipids found in strain DS22…”