Rho4 interaction with exocyst and septins regulates cell separation in fission yeast

Pérez, Pilar; Portales, Elvira; Santos, Beatriz Tamargo

doi:10.1099/mic.0.000062

Cited by 25 publications

(46 citation statements)

References 46 publications

(57 reference statements)

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“…C, Table ; Wang et al , ; Martin‐Cuadrado et al , ; Dekker et al , ; Garcia et al , ; Martin‐Cuadrado et al , ; Martin‐Cuadrado et al , ; Roncero and Sanchez, ; Sipiczki et al , ). The delivery of the glucanases are dependent on the exocyst complex (Wang et al , ; Wang et al , ; Martin‐Cuadrado et al , ; Perez et al , ). The exocyst complex is essential for viability; however, temperature sensitive sec8‐1 exocyst mutants and germinated spores of deletion mutants show polarized cell shape, indicating that it is not vital to cell polarity (Wang et al , ; Bendezu and Martin, ).…”

Section: Membrane Trafficking Events During Cytokinesismentioning

confidence: 99%

“…In addition to the exocyst complex, delivery of the hydrolytic enzymes required for septum digestion also depends on the Rho3/4 GTPases (Wang et al , ; Wang et al , ; Perez et al , ; Wang et al , ). Rho3 promotes the delivery of secretory vesicles to the delivery site, likely through interaction with the exocyst complex (Wang et al , ).…”

Section: Membrane Trafficking Events During Cytokinesismentioning

confidence: 99%

“…When exocyst functionality is compromised, Rho3 localization to the division site is impaired (Wang et al , ). In contrast, Rho4 localization is independent of the exocyst complex but the exocyst subunit Sec8 fails to localize to the division site in rho4 mutants (Perez et al , ). The Rho GEF, Gef3 also contributes to cell separation, likely via Rho4 activation (Wang et al , ).…”

Section: Membrane Trafficking Events During Cytokinesismentioning

confidence: 99%

“…While the authors report that addition of the drug aculeacin A (blocks glucan synthesis) does not lead to ring sliding, Bgs1 is resistant to this drug and thus one cannot conclude if the Bgs1 enzymatic activity is necessary for anchoring the ring (Castro et al, 1995;Martins et al, 2011). Bgs1 has been reported to cooperate with Paxillin Pxl1 and Cdc15 to maintain ring integrity and to Martin-Cuadrado et al (2003), Wang et al (2002), Garcia et al (2005), Sipiczki et al (2014), Martin-Cuadrado et al (2008), Wang et al (2003;2015), Perez et al (2015), Santos et al (2005), Munoz et al (2014), Villalobos-Duno et al (2013), Nakano et al (2003) promote septum synthesis (Cortes et al, 2015). Similar to cdc15 and bgs1 mutants, pxl1 mutants also show ring sliding defects (Cortes et al, 2015).…”

Section: Septum-driven Cytokinesis In Fission Yeastmentioning

confidence: 99%

“…Septin protein complexes are also required for the delivery of glucanases to the division site. This includes the anillin homolog Mid2 and the septin proteins Spn1-4 (Tasto et al, 2003;An et al, 2004;Martin-Cuadrado et al, 2005;Sipiczki, 2007;Roncero and Sanchez, 2010;Wu et al, 2010;Munoz et al, 2014;Perez et al, 2015;Wang et al, 2015;Zheng et al, 2018). Mid2 organizes the septin ring that forms on either side of the septum barrier (Tasto et al, 2003).…”

Section: Septum Digestion and Cell Separationmentioning

confidence: 99%

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Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

Hercyk

Onwubiko

Das

2019

Molecular Microbiology

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Summary During cytokinesis, animal and fungal cells form a membrane furrow via actomyosin ring constriction. Our understanding of actomyosin ring‐driven cytokinesis stems extensively from the fission yeast model system. However, unlike animal cells, actomyosin ring constriction occurs simultaneously with septum formation in fungi. While the formation of an actomyosin ring is essential for cytokinesis in fission yeast, proper furrow formation also requires septum deposition. The molecular mechanisms of spatiotemporal coordination of septum deposition with actomyosin ring constriction are poorly understood. Although the role of the actomyosin ring as a mechanical structure driving furrow formation is better understood, its role as a spatiotemporal landmark for septum deposition is not widely discussed. Here we review and discuss the recent advances describing how the actomyosin ring spatiotemporally regulates membrane traffic to promote septum‐driven cytokinesis in fission yeast. Finally, we explore emerging questions in cytokinesis, and discuss the role of extracellular matrix during cytokinesis in other organisms.

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Section: Membrane Trafficking Events During Cytokinesismentioning

confidence: 99%

Section: Membrane Trafficking Events During Cytokinesismentioning

confidence: 99%

Section: Membrane Trafficking Events During Cytokinesismentioning

confidence: 99%

Section: Septum-driven Cytokinesis In Fission Yeastmentioning

confidence: 99%

Section: Septum Digestion and Cell Separationmentioning

confidence: 99%

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Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

Hercyk

Onwubiko

Das

2019

Molecular Microbiology

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Functional interaction between Cdc42 and the stress MAPK signaling pathway during the regulation of fission yeast polarized growth

et al. 2019

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Cell polarization can be defined as the generation and maintenance of directional cellular organization. The spatial distribution and protein or lipid composition of the cell are not symmetric but organized in specialized domains which allow cells to grow and acquire a certain shape that is closely linked to their physiological function. The establishment and maintenance of polarized growth requires the coordination of diverse processes including cytoskeletal dynamics, membrane trafficking, and signaling cascade regulation. Some of the major players involved in the selection and maintenance of sites for polarized growth are Rho GTPases, which recognize the polarization site and transmit the signal to regulatory proteins of the cytoskeleton. Additionally, cytoskeletal organization, polarized secretion, and endocytosis are controlled by signaling pathways including those mediated by mitogen-activated protein kinases (MAPKs). Rho GTPases and the MAPK signaling pathways are strongly conserved from yeast to mammals, suggesting that the basic mechanisms of polarized growth have been maintained throughout evolution. For this reason, the study of how polarized growth is established and regulated in simple organisms such as the fission yeast Schizosaccharomyces pombe has contributed to broaden our knowledge about these processes in multicellular organisms. We review here the function of the Cdc42 GTPase and the stress activated MAPK (SAPK) signaling pathways during fission yeast polarized growth, and discuss the relevance of the crosstalk between both pathways.

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The price of independence: cell separation in fission yeast

Martín‐García

Santos

2016

World J Microbiol Biotechnol

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The ultimate goal of cell division is to give rise to two viable independent daughter cells. A tight spatial and temporal regulation between chromosome segregation and cytokinesis ensures the viability of the daughter cells. Schizosaccharomyces pombe, commonly known as fission yeast, has become a leading model organism for studying essential and conserved mechanisms of the eukaryotic cell division process. Like many other eukaryotic cells it divides by binary fission and the cleavage furrow undergoes ingression due to the contraction of an actomyosin ring. In contrast to mammalian cells, yeasts as cell-walled organisms, also need to form a division septum made of cell wall material to complete the process of cytokinesis. The division septum is deposited behind the constricting ring and it will constitute the new ends of the daughter cells. Cell separation also involves cell wall degradation and this process should be precisely regulated to avoid cell lysis. In this review, we will give a brief overview of the whole cytokinesis process in fission yeast, from the positioning and assembly of the contractile ring to the final step of cell separation, and the problems generated when these processes are not precise.

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Rho4 interaction with exocyst and septins regulates cell separation in fission yeast

Cited by 25 publications

References 46 publications

Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

Functional interaction between Cdc42 and the stress MAPK signaling pathway during the regulation of fission yeast polarized growth

The price of independence: cell separation in fission yeast

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