Rhizosphere microorganisms enhance in vitro root and plantlet development of Pyrus and Prunus rootstocks

Cantabella, Daniel; Teixidó, Neus; Segarra, Guillem; Torres, Rosário; Casanovas, María; Dolcet-Sanjuan, Ramón

doi:10.1007/s00425-021-03595-3

Cited by 15 publications

(15 citation statements)

References 46 publications

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“…of their differential regenerative responses to be identified. In addition, our results from substrate and hydroponic system experiments confirmed that adventitious rooting of hardwood cuttings was dependent on both genotype and environment factors [ 22 , 30 , 31 ], and as such, these two factors must be considered during rootstock breeding.…”

Section: Discussionsupporting

confidence: 60%

“…Despite the availability of effective protocols for vegetative multiplication in most woody plants, the rooting efficiency of some Prunus sp. clones is problematic [ 22 ]. Rootstocks are an essential component of modern fruit agriculture due to their ability to adapt a particular crop to various environmental conditions and cultural practices [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Justamante

Mhimdi

Molina-Pérez

et al. 2022

Plants

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Several Prunus species are among the most important cultivated stone fruits in the Mediterranean region, and there is an urgent need to obtain rootstocks with specific adaptations to challenging environmental conditions. The development of adventitious roots (ARs) is an evolutionary mechanism of high relevance for stress tolerance, which has led to the development of environmentally resilient plants. As a first step towards understanding the genetic determinants involved in AR formation in Prunus sp., we evaluated the rooting of hardwood cuttings from five Prunus rootstocks (Adafuel, Adarcias, Cadaman, Garnem, and GF 677) grown in hydroponics. We found that auxin-induced callus and rooting responses were strongly genotype-dependent. To investigate the molecular mechanisms involved in these differential responses, we performed a time-series study of AR formation in two rootstocks with contrasting rooting performance, Garnem and GF 677, by culturing in vitro microcuttings with and without auxin treatment (0.9 mg/L of indole-3-butyric acid [IBA]). Despite showing a similar histological structure, Garnem and GF677 rootstocks displayed dynamic changes in endogenous hormone homeostasis involving metabolites such as indole-3-acetic acid (IAA) conjugated to aspartic acid (IAA-Asp), and these changes could explain the differences observed during rooting.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Justamante

Mhimdi

Molina-Pérez

et al. 2022

Plants

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“…Previous studies have shown that the presence of P. oryzihabitans PGP01 induces modifications in Pyrus and Prunus rootstocks ( Cantabella et al , 2020 , 2021 ). The data presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots toPseudomonas oryzihabitans

Cantabella

Karpińska

Teixidó

et al. 2022

Journal of Experimental Botany

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Soil bacteria promote plant growth and protect against environmental stresses but the mechanisms involved remain poorly characterised, particularly when there is no direct contact between the roots and bacteria. Here, we explored the effects of Pseudomonas oryzihabitans PGP01 on the root system architecture (RSA) in Arabidopsis thaliana seedlings. Significant increases in lateral root (LR) density were observed when seedlings were grown in the presence of P. oryzihabitans, as well as an increased abundance of transcripts associated with altered nutrient transport and phytohormone responses. However, no bacterial transcripts were detected on the root samples by RNAseq analysis, demonstrating that the bacteria do not colonise the roots. Separating the agar containing bacteria from the seedlings prevented the bacteria-induced changes in RSA. Bacteria-induced changes in RSA were absent from mutants defective in Ethylene Response Factor (ERF109), glutathione synthesis (pad2-1, cad2-1 and rax1-1) and in strigolactone (SL) synthesis (max3-9 and max4-1) or signalling (max2-3). However, the P. oryzihabitans-induced changes in RSA were similar in the low ascorbate mutants (vtc2-1, vtc2-2) to the wild type controls. Taken together, these results demonstrate the importance of non-volatile signals and redox mechanisms in the root architecture regulation that occurs following long-distance perception of P. oryzihabitans.

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“…Contrarily, C. ramotenellum is mostly associated with plants but presents a dual nature. On one hand, the species has been described as a beneficial mycorrhizal fungus [ 101 , 102 ], but it has also as been the cause of ripening in grape berries and mandarins [ 103 , 104 ]. The presence of C. allicinum and C. ramotenellum might be associated with migrating birds, which arrive for the breeding period on the nearby coast (Wedel Jarlsberg Land) [ 105 ].…”

Section: Discussionmentioning

confidence: 99%

The First Comprehensive Biodiversity Study of Culturable Fungal Communities Inhabiting Cryoconite Holes in the Werenskiold Glacier on Spitsbergen (Svalbard Archipelago, Arctic)

Borzęcka

Suchodolski

Dudek

et al. 2022

Biology

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Cryoconite holes on glacier surfaces are a source of cold-adapted microorganisms, but little is known about their fungal inhabitants. Here, we provide the first report of distinctive fungal communities in cryoconite holes in the Werenskiold Glacier on Spitsbergen (Svalbard Archipelago, Arctic). Due to a combination of two incubation temperatures (7 °C and 24 ± 0.5 °C) and two media during isolation (PDA, YPG), as well as classical and molecular identification approaches, we were able to identify 23 different fungi (21 species and 2 unassigned species). Most of the fungi cultured from cryoconite sediment were ascomycetous filamentous micromycetes. However, four representatives of macromycetes were also identified (Bjerkandera adusta, Holwaya mucida, Orbiliaceae sp., and Trametes versicolor). Some of the described fungi possess biotechnological potential (Aspergillus pseudoglaucus, A. sydowii, Penicillium expansum, P. velutinum, B. adusta, and T. versicolor), thus, we propose the Arctic region as a source of new strains for industrial applications. In addition, two phytopathogenic representatives were present (P. sumatraense, Botrytis cinerea), as well as one potentially harmful to humans (Cladosporium cladosporioides). To the best of our knowledge, we are the first to report the occurrence of A. pseudoglaucus, C. allicinum, C. ramotenellum, P. sumatraense, P. velutinum, P. cumulodentata, B. adusta, and T. versicolor in polar regions. In all likelihood, two unassigned fungus species (Orbiliaceae and Dothideomycetes spp.) might also be newly described in such environments. Additionally, due to experimenting with 10 sampling sites located at different latitudes, we were able to conclude that the number of fungal spores decreases as one moves down the glacier. Considering the prevalence and endangerment of glacial environments worldwide, such findings suggest their potential as reservoirs of fungal diversity, which should not be overlooked.

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Rhizosphere microorganisms enhance in vitro root and plantlet development of Pyrus and Prunus rootstocks

Cited by 15 publications

References 46 publications

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks

Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots toPseudomonas oryzihabitans

The First Comprehensive Biodiversity Study of Culturable Fungal Communities Inhabiting Cryoconite Holes in the Werenskiold Glacier on Spitsbergen (Svalbard Archipelago, Arctic)

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