Rhizobacteria Pseudomonas fluorescens and Azospirillum sp. association enhances growth of Lactuca sativa L. under tropical conditions

Aponte, A P Freddy; Castillo, O.; Cabrera, Ganimedes; Pernia, Mariloly; Hernández, Y.

doi:10.5513/jcea01/18.2.1916

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…Pseudomonas putida and P. fluorescens are saprophytic bacteria (Lelliot & Stead, 1987) commonly present in the soil in Brazil (Cabral et al, 2013;Ferraz et al, 2015;Aponte et al, 2017;Sousa et al, 2019), but until now, no phytopathogenic activity associated with these species has been described, and also have not been described in the Absent Quarantine Pest List (A1) of the Ministry of Agriculture, Livestock and Food Supply (Mapa, 2018). These species have already been reported to cause pith necrosis in Europe (Lo Cantore & Iacobellis, 2002;Saygili et al, 2004;Polizzi et al, 2007;Dimartino et al, 2011), although the bacteria transmission by the seeds was not described up to now.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

2021

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In 2016, several reports emerged of fresh-market staked tomato plants with severe symptoms of pith necrosis and premature death in commercial cultivation areas in the states of Paraná and Minas Gerais, Brazil, which are similar to those caused by Pseudomonas corrugata. Four bacterial strains were isolated from infected tissue samples and characterized as Gram-negative, aerobic, and fluorescent on King’s B Medium. LOPAT tests were performed and the isolate UFU H120 was classified into group Vb (+ + − + −) and the isolates UFU H3, UFU H6, and UFU H21 into group Va (− + − + −). The pathogenicity of the isolates was confirmed by artificial inoculation on tomato plants of Santa Cruz type, cv. Kada Gigante, and Koch’s postulates were accomplished successfully. The isolates sequences of their 16S rRNA gene region were compared with those deposited in GenBank, the isolate UFU H120 aligning with Pseudomonas fluorescens (99,45% similarity) and the isolates UFU H3, UFU H6, and UFU H21 with Pseudomonas putida (99.2, 99.53 and 99.64% similarity, respectively). Notably, P. fluorescens and P. putida are most known as saprophytic bacteria normally present in the soil, although both of these species have already been reported to infect tomato plants in Italy. However, so far, there have not been any reports of such bacteria being phytopathogenic on tomato or any crop in Brazil. Thus, to our knowledge, this is the first report of pith necrosis of such occurrence in Brazil.

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Section: Resultsmentioning

confidence: 99%

Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

2021

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“…Working with lettuce in tropical conditions, Aponte et al (2017) found that the association of Azospirillum with Pseudomonas fluorescens promoted greater seedling emergence, greater leaf area, and greater dry mass, highlighting the advantages of the bio-consortium of strains. According to the same authors, these microorganisms act synergistically and can trigger a tolerance mechanism for lettuce under abiotic stress conditions.…”

Section: Stems Leaves and Bulbsmentioning

confidence: 98%

“…The Azospirillum genus falls within the group of plant growth-promoting rhizobacteria (PGPRs) (Kloepper & Schroth, 1978). Although it has stood out for a longer time in use in species from the Poaceae family, it started to interest the horticultural sector for the possible benefits provided, such as reduced use of nitrogen (Lima et al, 2017;Aguirre et al, 2018), increased yield (Aponte et al, 2017) and product quality, among others. Still, it highlights the interest in producing organic vegetables as a good option for crop management.…”

Section: Stems Leaves and Bulbsmentioning

confidence: 99%

The Azospirillum genus and the cultivation of vegetables. A review

Vendruscolo¹,

Lima²

2021

Biotechnol. Agron. Soc. Environ.

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Introduction. Plant growth-promoting rhizobacteria have been successfully inserted in agriculture, aiming to find a better balance between agricultural production and environmental protection. However, there is a restriction on its application concerning the exploited species since the focus is on large-scale grain production, practically excluding small and medium-sized farms. Literature. Studies on the application of Azospirillum bacteria in horticultural species are still scarce, compared with those aimed at grain production. However, it appears that these bacteria are beneficial to the development and production of vegetables, whether they produce leaves, stems, bulbs, flowers, fruits, roots, or tubers, and may result in monetary gains, especially for small and medium agricultural producers. Conclusions. The use of Azospirillum bacteria to increase the quality and quantity of products from horticultural species and establish an environmentally friendly practice is a reality. However, the development of research that defines the best strategies for using this technology must be carried out continuously, aiming at the best conditions for producers.

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“…This antibiotic is active mainly against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus [ 12 ]. All these properties make P. fluorescens interesting in the field of biotechnology [ 13 ], with multiple applications in food, agriculture, health and environment [ 14 ]. At the same time, the status of P. fluorescens as a non-pathogenic bacterium can be questioned, with some strains being able to act as pathogens under specific conditions [ 15 ] [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Growth Conditions on Pseudomonas fluorescens Morphology Characterized by Atomic Force Microscopy

Kahli

Béven

Grauby–Heywang

et al. 2022

IJMS

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This work is dedicated to the characterization by Atomic Force Microscopy (AFM) of Pseudomonas fluorescens, bacteria having high potential in biotechnology. They were first studied first in optimal conditions in terms of culture medium and temperature. AFM revealed a more-or-less elongated morphology with typical dimensions in the micrometer range, and an organization of the outer membrane characterized by the presence of long and randomly distributed ripples, which are likely related to the organization of lipopolysaccharides (LPS). The outer membrane also presents invaginations, some of them showing a reorganization of ripples, which could be the first sign of a bacterial stress response. In a second step, bacteria grown under unfavorable conditions were characterized. The choice of the medium appeared to be more critical in the case of the second generation of cells, the less adapted medium inducing not only changes in the membrane organization but also larger damages in bacteria. An increased growth temperature affected both the usual “swollen” morphology and the organization of the outer membrane. Here also, LPS likely contribute to membrane remodelling, which makes them potential markers to track cell state changes.

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Rhizobacteria Pseudomonas fluorescens and Azospirillum sp. association enhances growth of Lactuca sativa L. under tropical conditions

Cited by 9 publications

References 35 publications

Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

Occurrence of Pseudomonas fluorescens and P. putida associated to tomato pith necrosis in Brazil

The Azospirillum genus and the cultivation of vegetables. A review

Impact of Growth Conditions on Pseudomonas fluorescens Morphology Characterized by Atomic Force Microscopy

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