rhBMP2-loaded calcium phosphate cements combined with allogenic bone marrow mesenchymal stem cells for bone formation

Luo, Gaoxing; Huang, Yadong; Gu, Fei

doi:10.1016/j.biopha.2017.05.083

Cited by 14 publications

(9 citation statements)

References 32 publications

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“…BMP-2, part of the transforming growth factorbeta superfamily, is involved in induction of differentiation of bone and cartilage. Triggering of BMP-2 enhances the osteogenic differentiation of BMSCs (Luo, Huang, & Gu, 2017;Um et al, 2017). We F I G U R E 6 The effect of BMP-2 on FD-BMSCs and normal BMSCs according to transplantation ratio.…”

Section: Discussionmentioning

confidence: 99%

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Effects of BMP‐2 on the osteogenic differentiation of bone marrow stem cells in fibrous dysplasia

Seo

2018

Oral Diseases

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Section: Discussionmentioning

confidence: 99%

“…BMP‐2, part of the transforming growth factor‐beta superfamily, is involved in induction of differentiation of bone and cartilage. Triggering of BMP‐2 enhances the osteogenic differentiation of BMSCs (Luo, Huang, & Gu, ; Um et al., ). We found that 200 ng/ml BMP‐2 increased the osteogenic differentiation potential of FD‐BMSCs.…”

Section: Discussionmentioning

confidence: 99%

Effects of BMP‐2 on the osteogenic differentiation of bone marrow stem cells in fibrous dysplasia

Seo

2018

Oral Diseases

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“…In addition, as an ideal support, Bio-Oss is similar to the human bone structure and has a large internal surface area, which makes it suitable for the adsorption of growth factors and biological proteins (28). The different pore sizes of the internal structure of Bio-Oss can promote the regeneration of blood vessels and maintain the stability of blood clots (29). Bio-Oss consists of small granules, with a diameter of 10–60 nm.…”

Section: Discussionmentioning

confidence: 99%

Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

et al. 2019

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Hypoxia-inducible factor (HIF)-1α protein, which is upregulated by hypoxia, serves an important role in angiogenesis during osteogenesis. The aim of the present study was to investigate the effect of HIF-1α on alveolar ridge preservation in a dog tooth extraction model. Six beagle dogs were used in the present study. The second and fourth premolar teeth of the lower jaws on both sides were extracted. Two unilateral extraction sockets were randomly selected and filled with Bio-Oss and Bio-Oss + HIF-1α. The contralateral sockets remained unfilled and served as the negative control. Micro-computed tomography examination and histological staining were performed to examine the difference of new bone formation among the three groups. Western blotting and reverse transcription-quantitative polymerase chain reaction analysis were used to detect the expression levels of osteogenesis- and angiogenesis-associated genes in the bone tissues of the three groups. Twelve weeks post-surgery, trabecular bone formation in the Bio-Oss + HIF-1α group was significantly increased compared with the other groups. The expression levels of osteogenesis-associated genes (runt-related transcription factor 2, osteoblast-specific transcription factor osterix and osteocalcin) and angiogenesis-associated genes (HIF-1α and vascular endothelial growth factor) were all significantly increased in the Bio-Oss + HIF-1α group compared with the other two groups (P<0.05). The present results indicated that Bio-Oss with HIF-1α can promote osteogenesis and angiogenesis in vivo and may be used as an effective treatment for the preservation of the alveolar ridge.

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“…BMSCs are pluripotent stromal cells that are easily isolated and cultured in vitro, 8,9 which has osteogenic differentiation potential, and strong proliferative capacity. [10][11][12] It has been demonstrated in many studies that BMSCs plays an important role in bone formation 13 and is therefore considered to be a good seed cell for bone tissue engineering, 14 as well as one of the most important cellular sources of regenerative medicine. 15 The methods of obtaining BMSCs mainly include flow cytometry separation, whole bone marrow culture and density gradient centrifugation combined with adherent culture.…”

Section: Discussionmentioning

confidence: 99%

Effects of lentiviral transfection containing bFGF gene on the biological characteristics of rabbit BMSCs

Peng

Zhang

et al. 2018

J of Cellular Biochemistry

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To investigate the effect of lentiviral transfection containing bFGF gene on the biological characteristics of bone marrow stromal cells (BMSCs). BMSCs were obtained by density gradient centrifugation and adherence screening. The bFGF gene was transfected into BMSCs by lentiviral vector and divided into bFGF transfection group, empty virus group, and untransfected group according to the transfection conditions. After transfection, the morphology, expression of bFGF mRNA and protein, cell proliferation, cell cycle, and alkaline phosphatase (ALP) activity were observed in three groups of cells. High density BMSCs were successfully obtained by density gradient centrifugation and adherence screening. After transfection of BMSCs with bFGF gene, the cell morphology did not change significantly, while the expression of bFGF mRNA and protein were significantly increased, the cell proliferation curve shifted upward, the proportion of proliferating cells increased, and the activity of ALP was significantly enhanced. Compared with empty virus group and untransfected group, there was significant difference (P < 0.05). The rabbit bFGF gene was successfully introduced into the BMSCs cultured in vitro by lentiviral vector, and the target gene was stably expressed. And the expression of bFGF can promote the proliferation and osteogenic differentiation of BMSCs.

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rhBMP2-loaded calcium phosphate cements combined with allogenic bone marrow mesenchymal stem cells for bone formation

Cited by 14 publications

References 32 publications

Effects of BMP‐2 on the osteogenic differentiation of bone marrow stem cells in fibrous dysplasia

Effects of BMP‐2 on the osteogenic differentiation of bone marrow stem cells in fibrous dysplasia

Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

Effects of lentiviral transfection containing bFGF gene on the biological characteristics of rabbit BMSCs

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