Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

Tan, Luanjun; Zhang, Yingdi; Huang, Yadong; Luo, Yuan; Liu, Yuehua

doi:10.3892/etm.2019.7301

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…In the process of bone regeneration, HIF-1α not only promotes angiogenesis but also regulates metabolic adaptations by inducing glycolysis transformation to promote cell survival [ 30 ]. Hence, HIF-1 serves an indispensable role in osteogenesis and bone restoration [ 26 , 31 ]. When osteoblasts and other associated cells sense reduced oxygen tension, intracellular HIF-1α is stably expressed to regulate the expression of the angiogenic and osteogenic genes [ 32 ].…”

Section: Hypoxia-inducible Factorsmentioning

confidence: 99%

Hypoxia-Inducible Factors Signaling in Osteogenesis and Skeletal Repair

Qin

Liu²,

Yang³

et al. 2022

IJMS

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Sufficient oxygen is required to maintain normal cellular and physiological function, such as a creature’s development, breeding, and homeostasis. Lately, some researchers have reported that both pathological hypoxia and environmental hypoxia might affect bone health. Adaptation to hypoxia is a pivotal cellular event in normal cell development and differentiation and in pathological settings such as ischemia. As central mediators of homeostasis, hypoxia-inducible transcription factors (HIFs) can allow cells to survive in a low-oxygen environment and are essential for the regulation of osteogenesis and skeletal repair. From this perspective, we summarized the role of HIF-1 and HIF-2 in signaling pathways implicated in bone development and skeletal repair and outlined the molecular mechanism of regulation of downstream growth factors and protein molecules such as VEGF, EPO, and so on. All of these present an opportunity for developing therapies for bone regeneration.

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Section: Hypoxia-inducible Factorsmentioning

confidence: 99%

Hypoxia-Inducible Factors Signaling in Osteogenesis and Skeletal Repair

Qin

Liu²,

Yang³

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Hypoxia-inducible factor HIF-1α, which is induced by hypoxia, plays a crucial role in angiogenesis. Human hematological malignant disease, including leukemia and lymphoma, express high levels of HIF-1α, which is often closely associated with poor disease prognosis (16,17). However, there are few researches exploring the relationship between cancers and HIF1α in the veterinary field (18).…”

Section: Introductionmentioning

confidence: 99%

A Novel Canine Mammary Cancer Cell Line: Preliminary Identification and Utilization for Drug Screening Studies

Sun

et al. 2021

Front. Vet. Sci.

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Canine malignant mammary tumor is a dangerously fatal neoplastic disease with poor survival in female dogs. The aim of this study was to preliminary characterize a novel canine mammary cancer cell line, B-CMT, from canine primary mammary gland tumor, and to utilize it as a cell model for in vitro screening of possible therapeutic drugs. The successfully established cell line, B-CMT, was cultured over 50 passages. B-CMT has a fast proliferation rate, and a population doubling time (PDT) of 33.6 h. The B-CMT cell line lacked human epidermal growth factor receptor-2 (HER-2), estrogen receptors (ER) and progesterone receptors (PR) expression by qRT-PCR. Compared with MDCK cells, CDH1 expression of CMT cell line was significantly decreased or even absent, but GATA3 expression dramatically increased, while TGF-β expression was at a similar level. Interestingly, the B-CMT cell line from canine primary tumor also showed positive hypoxia inducible factor-1α (HIF-1α) results in immunofluorescence (IF), western blot, and qRT-PCR analysis. Ten days post inoculation with EGFP-B-CMT (B-CMT cells stably expressing EGFP), the experimental mice developed palpable soft tissue masses which histologically resembled the canine primary tumor, and was approved to be derived from B-CMT cell line through detection of EGFP by immunohistochemical (IHC) analysis. Moreover, we investigated the cytotoxicity of five drugs to B-CMT cells, and the results showed that rapamycin and imatinib significantly inhibited the proliferation of the cells in vitro within a certain range of concentration. They also induced cell cycle arrest of B-CMT cells at G1 and G2 phase, respectively. In summary, the results of this report showed that B-CMT cell line might serve as a tool for future studies on tumor microenvironment and drug resistance.

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Characterizations of alveolar repair after mandibular second molar extraction: an experimental study in rats

Sheng

Sun

et al. 2022

J. Appl. Oral Sci.

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Characterizations of rat mandibular second molar extraction socket with significantly different buccal and lingual alveolar ridge width remain unclear. Objective: To observe alterations in the alveolar ridge after extraction of mandibular second molars, and to examine processes of alveolar socket healing in an experimental model of alveolar ridge absorption and preservation. Methodology: Eighteen Wistar rats were included and divided into six groups regarding healing time in the study. Bilateral mandibular second molars were extracted. The rats with tooth extraction sockets took 0, 1.5, 2, 3, 4 and 8 weeks of healing. Histological observation, tartrate-resistant acidic phosphatase (TRAP) staining, Masson’s trichrome staining, immunohistochemical staining and micro-computed tomography (micro-CT) were applied to estimate alterations in the alveolar ridge. Results: Different buccal and lingual alveolar ridge width led to different height loss. Lingual wall height (LH) decreased significantly two weeks after tooth extraction. Buccal wall height rarely reduced its higher ridge width. From two to eight weeks after extraction, bone volume (BV/TV), density (BMD), and trabecular thickness (Tb.Th) progressively increased in the alveolar socket, which gradually decreased in Tb.Sp and Tb.N. LH showed no significant change during the same period. Osteogenic marker OCN and OPN increased during bone repair from two to eight weeks. The reduced height of the lingual wall of the tooth extraction socket was rarely repaired in the later repair stage. Osteoclast activity led to absorption of the alveolar ridge of the alveolar bone wall within two weeks after operation. We observed positive expression of EMMPRIN and MMP-9 in osteoclasts that participated in the absorption of the spire region. Conclusion: Extraction of rat mandibular second molars may help the study of alveolar ridge absorption and preservation. The EMMPRIN-MMP-9 pathway may be a candidate for further study on attenuating bone resorption after tooth extraction.

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Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

Cited by 4 publications

References 35 publications

Hypoxia-Inducible Factors Signaling in Osteogenesis and Skeletal Repair

Hypoxia-Inducible Factors Signaling in Osteogenesis and Skeletal Repair

A Novel Canine Mammary Cancer Cell Line: Preliminary Identification and Utilization for Drug Screening Studies

Characterizations of alveolar repair after mandibular second molar extraction: an experimental study in rats

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