Review: role of carbon sources for in vitro plant growth and development

Yaseen, Mehwish; Ahmad, Touqeer; Sablok, Gaurav; Standardi, A.; Hafiz, Ishfaq Ahmad

doi:10.1007/s11033-012-2299-z

Cited by 180 publications

(115 citation statements)

References 124 publications

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“…Sugars are necessary for the function of several parts of morphogenesis, such as the osmolarity balance of the culture medium, skeletal function for the building of various important factors, heterotrotophy/mixotrophy, and regulation of genes responsible for plant growth (Yaseen et al, 2013). Some studies on plants from the Zingiberaceae family have shown that higher sucrose concentrations of 60 to 70 g/L are more efficient for in vitro plant morphogenesis when compared to the usual concentration of 30 g/L.…”

Section: Effect Of Activated Charcoal and Sucrosementioning

confidence: 99%

“…Beyond sucrose, other carbon sources have been used in the micropropagation, as is the case of sorbitol, mannitol, fructose, galactose (pentoses) and maltose (disaccharide) (Yaseen et al, 2013). Being the first two, more are used in essays involving minimal cultivation or in germplasm collection establishment (Martins et al, 2011).…”

Section: Effect Of Activated Charcoal and Sucrosementioning

confidence: 99%

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Evaluation of new protocols to Curcuma longa micropropagation: A medicinal and ornamental specie

Ferrari¹,

Antoniazzi²,

Nascimento³

et al. 2016

J. Med. Plants Res.

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Turmeric (Curcuma longa) is an Asian native species used for ornamental, medicinal and food purposes. However, its conventional propagation method is costly and inefficient. Therefore, this study aimed to verify appropriate asepsis methods for shoots from rhizomes, evaluate concentrations of activated charcoal and sucrose, and determine appropriate salt concentrations in Murashige and Skoog (MS) medium for turmeric growth. Three independent assays were performed. First, asepsis methods were verified for explants; second was for concentrations of sucrose and activated charcoal and in the final assay salt concentrations was evaluated. In all assays, MS medium supplemented with 30 g/L of sucrose, 6.5 g/L of agar and pH adjusted to 5.8 was used. Two growth regulators were added to the culture medium: 4.44 µM of 6-benzyl-amino-purine (BAP) and 1.08 µM of α-naphthalene acetic acid (NAA). Data obtained were subjected to analysis of variance (ANOVA) at p ≤ 0.05 probability. The averages were compared by Tukey's test and polynomial regression at p ≤ 0.05. All the asepsis methods evaluated showed similar effects. The highest concentration was obtained by utilizing 60 mg/L sucrose for the most part of the evaluated characteristics; however, it is recommended that activated charcoal be used at a concentration of 4.5 g/L. For salt concentrations, averages were compared by polynomial regression at (p ≤ 0.05) probability in 50 to 60% MS medium, and resulted in longer root systems, greater numbers of shoots and more leaves. The usual dose of 3% sucrose resulted in lower development of in vitro seedlings. Salt concentrations higher than 60% were toxic to turmeric tissues and consequently compromised the root system and the aerial part of developing seedlings.

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Section: Effect Of Activated Charcoal and Sucrosementioning

confidence: 99%

Section: Effect Of Activated Charcoal and Sucrosementioning

confidence: 99%

Evaluation of new protocols to Curcuma longa micropropagation: A medicinal and ornamental specie

Ferrari¹,

Antoniazzi²,

Nascimento³

et al. 2016

J. Med. Plants Res.

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show abstract

“…Moreover, the role of LEAFY COTYLEDON1 (LEC1) gene, that controls embryogenic competence, requires both the auxin and sucrose to promote cell division and embryonic differentiation (Casson and Lindsey 2006). Various exogenous sugars have been used to enhance SE efficiency, indicating that carbon sources play an important role in this process (Yaseen et al 2013). Despite the importance of sucrose metabolism in maintaining the balance between hexose signals and metabolic paths (Koch 2004), there is only one example of the relationship between the content of phytohormones and endogenous sugars during the SE induction currently displayed .…”

Section: Introductionmentioning

confidence: 99%

Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb.

2017

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Somatic embryogenesis (SE) in the tree fern Cyathea delgadii was first described in 2015 and since then has been used to exploration of this phenomenon in cryptogamic plants. To deepen the knowledge about the hormonal control of SE, stipe explants were cultured on media supplemented with hormone biosynthesis and transport inhibitors (HBTIs). In the presence of 30 µM 2,3,5-triiodobenzoic acid (TIBA), or 40 µM fluridone or 125 µM salicylic acid (SA), somatic embryo production was totally inhibited. The quantitative analysis of the changes in endogenous hormone and sugar contents was conducted every 2 days within 10-day-long initial culture. The results showed that the concentrations of endogenous indole-3-acetic acid (IAA), abscisic acid (ABA), cytokinins (CKs) and soluble sugars were strongly modified either by TIBA and fluridone. Under their influence, the contents of cytokinins such as c-Z, c-ZR, t-Z, t-ZR, KinR were reduced to barely detectable levels. Treatment with SA results in the changes in endogenous IAA and sugar contents. It also modifies the IAA/CKs ratio; however, excluding the first 2 days of culture, the concentrations of ABA and cytokinins were kept on the control level. All HBTIs significantly increased the kinetin (Kin) content. Our work sheds new light on the relationships between the biosynthetic inhibitors and phytohormones and sugars in the process of early SE. It can be helpful to study the role of hormones in acquisition of embryogenic competence.

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“…No cultivo in vitro de plantas a fotossíntese é mínima, pois a plântula em desenvolvimento tem suas necessidades de carbono supridas pela adição de sacarose no meio de cultura (YASEEN et al, 2013;GAGO et al, 2014). Já o fototropismo, também não é tão relevante nas condições in vitro, no entanto, em relação à fotomorfogênese a luz tem papel direto nas respostas morfofisiológicas do vegetal, portanto, interessa a elucidação de sua influência sobre as plântulas em crescimento (GEORGE et al, 2008).…”

Section: Introductionunclassified

ESPECTROS LUMINOSOS NO DESENVOLVIMENTO DE PLÂNTULAS DE Curcuma longa CULTIVADAS IN VITRO

Ferrari

Antoniazzi

Nascimento

et al. 2017

ArqVet

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Umuarama, v. 19, n. 4, p. 247-251, out./dez. 2016. RESUMO:Curcuma longa é uma espécie asiática perene e rizomatosa com grandes propriedades medicinais, alimentícias e ornamentais. No processo micropropagativo carece de muitas informações dentre a faixa espectral mais adequada ao seu desenvolvimento. Sendo assim, o objetivo desse trabalho foi avaliar diferentes filmes espectrais no desenvolvimento de plântulas de C. longa cultivadas in vitro. Para tanto, brotações de C. longa foram inoculadas em meio Murashig e Skoog e suplementado com 30 g/L de sacarose, 6,5 g/L de ágar 4, 44 μM/L de Benzil aminopurina (BAP) e 1,08 μM/L ácido naftaleno acético (ANA). O pH do meio foi ajustado para 5,8. Os brotos foram submetidos a diferentes intensidades e qualidade espectral de luz: branco, vermelho, amarelo, azul e verde. As plântulas foram mantidas em sala de crescimento em luz constante de 25C° e fotoperíodo de 24 horas por 145 dias. Foram avaliadas características morfológicas e anatômicas. Os resultados obtidos demonstraram que as diferenças espectraris propiciaram diferenças no desenvolvimento das plântulas de C. longa e também na contaminação in vitro. O diâmetro da base das plântulas, a massa seca e fresca da parte área e raiz foram influenciadas pelas diferentes faixas espectrais, sendo que de modo geral os filmes, amarelo e branco foram os que propiciaram maiores valores para essas características. Já o filme verde foi o que menos favoreceu o ganho de massa das plântulas, além disso, alta taxa de contaminação foi observada na presença desse filme. PALAVRAS-CHAVE: Açafrão-da-terra. Filmes coloridos. Micropropagação. Morfologia. LUMINOUS SPECTRUM IN THE DEVELOPMENT OF IN-VITRO Curcuma longa SEEDLINGSABSTRACT: Curcuma longa is a perennial Asiatic rhizomelic species with important medicinal, food and ornamental properties. However, its micro-propagation process lacks information regarding the most adequate spectral range for its development. Thus, this work aimed to evaluate different spectrum films in the development of in vitro C. longa seedlings. In order to do this, C. longa sprouts were inoculated in Murashig and Skoog media supplemented with 30 g/L of sucrose, 6.5 g/L of agar 4, 44 μM/L of benzylaminopurine (BAP) and 1.08 μM/L naphthalene acetic acid (ANA). The pH was adjusted to 5.8. Sprouts were put under different light spectrum intensity and quality: white, red, yellow, blue and green. The seedlings were maintained in a growth room with constant light at 25 ºC and a 24-hours photoperiod for 145 days. Morphological and anatomical characteristics were analyzed. The results demonstrated that spectral differences propitiated differences in the development of C. longa seedlings, and also in the in vitro contamination. The seedling base diameter, aerial and root dry and fresh mass were influenced by the different spectral ranges, with the yellow and white ranges being those that resulting the highest values for each characteristic. The green spectrum was the least favorable for the seedling regarding mass gain, as we...

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Review: role of carbon sources for in vitro plant growth and development

Cited by 180 publications

References 124 publications

Evaluation of new protocols to Curcuma longa micropropagation: A medicinal and ornamental specie

Evaluation of new protocols to Curcuma longa micropropagation: A medicinal and ornamental specie

Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb.

ESPECTROS LUMINOSOS NO DESENVOLVIMENTO DE PLÂNTULAS DE Curcuma longa CULTIVADAS IN VITRO

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