ESPECTROS LUMINOSOS NO DESENVOLVIMENTO DE PLÂNTULAS DE Curcuma longa CULTIVADAS IN VITRO

Abstract

Umuarama, v. 19, n. 4, p. 247-251, out./dez. 2016. RESUMO:Curcuma longa é uma espécie asiática perene e rizomatosa com grandes propriedades medicinais, alimentícias e ornamentais. No processo micropropagativo carece de muitas informações dentre a faixa espectral mais adequada ao seu desenvolvimento. Sendo assim, o objetivo desse trabalho foi avaliar diferentes filmes espectrais no desenvolvimento de plântulas de C. longa cultivadas in vitro. Para tanto, brotações de C. longa foram inoculadas em meio Murashig e Skoog e suplementado com 30 g/L de sacarose, 6,5 g/L de ágar 4, 44 μM/L de Benzil aminopurina (BAP) e 1,08 μM/L ácido naftaleno acético (ANA). O pH do meio foi ajustado para 5,8. Os brotos foram submetidos a diferentes intensidades e qualidade espectral de luz: branco, vermelho, amarelo, azul e verde. As plântulas foram mantidas em sala de crescimento em luz constante de 25C° e fotoperíodo de 24 horas por 145 dias. Foram avaliadas características morfológicas e anatômicas. Os resultados obtidos demonstraram que as diferenças espectraris propiciaram diferenças no desenvolvimento das plântulas de C. longa e também na contaminação in vitro. O diâmetro da base das plântulas, a massa seca e fresca da parte área e raiz foram influenciadas pelas diferentes faixas espectrais, sendo que de modo geral os filmes, amarelo e branco foram os que propiciaram maiores valores para essas características. Já o filme verde foi o que menos favoreceu o ganho de massa das plântulas, além disso, alta taxa de contaminação foi observada na presença desse filme. PALAVRAS-CHAVE: Açafrão-da-terra. Filmes coloridos. Micropropagação. Morfologia. LUMINOUS SPECTRUM IN THE DEVELOPMENT OF IN-VITRO Curcuma longa SEEDLINGSABSTRACT: Curcuma longa is a perennial Asiatic rhizomelic species with important medicinal, food and ornamental properties. However, its micro-propagation process lacks information regarding the most adequate spectral range for its development. Thus, this work aimed to evaluate different spectrum films in the development of in vitro C. longa seedlings. In order to do this, C. longa sprouts were inoculated in Murashig and Skoog media supplemented with 30 g/L of sucrose, 6.5 g/L of agar 4, 44 μM/L of benzylaminopurine (BAP) and 1.08 μM/L naphthalene acetic acid (ANA). The pH was adjusted to 5.8. Sprouts were put under different light spectrum intensity and quality: white, red, yellow, blue and green. The seedlings were maintained in a growth room with constant light at 25 ºC and a 24-hours photoperiod for 145 days. Morphological and anatomical characteristics were analyzed. The results demonstrated that spectral differences propitiated differences in the development of C. longa seedlings, and also in the in vitro contamination. The seedling base diameter, aerial and root dry and fresh mass were influenced by the different spectral ranges, with the yellow and white ranges being those that resulting the highest values for each characteristic. The green spectrum was the least favorable for the seedling regarding mass gain, as we...

Physiological quality of cucurbits in spectral qualities

“…Positive results in terms of the increase in fresh shoot mass have already been reported for Curcuma longa in fluorescent light, followed by red light (~ 625-440 nm) and yellow light (~ 565-590nm; Ferrari et al, 2016). In contrast, Gu et al (2012), working with Anthurium andraeanum, noted the largest fresh mass with red + blue light, followed by white light.…”

“…The growth, development, production, and vigor of seedlings, and photosynthetic efficiency responses to spectral qualities have already been reported for other species (Ferrari et al, 2016). Although several authors have demonstrated the effects of spectral qualities on plant growth and development, the obtained responses vary for each crop (Dignart, 2006).…”

“…In their work with Anthurium andraeanum, Gu et al (2012) noted the highest root length under the red + blue LED and white LED. Ferrari et al (2016) noted the largest root length with blue LED followed by white LED. Su et al (2014) also reported that spectral red LED had a stimulating effect on root length.…”

Physiological characteristics of endive, lettuce and chicory seeds as a function of spectral quality

“…'Vera' lettuce had a decrease in root growth on the blue LED. This datum is in the counterhand to the description provided by Ferrari et al (2016) As to the fresh mass, lettuce seedlings showed the lowest averages, these in the qualities blue, white LED and fluorescent. The observation corroborates with Ferrari et al (2016).…”

“…The dry mass of saffron seedlings increased, as submitted to white and yellow spectra (Ferrari et al, 2016).…”