No abstract

Devido a relevante importância econômica, tanto para o setor alimentício quanto farmacêutico, a oliveira vem sendo cada vez mais cultivada em todo o mundo. Para isso, a obtenção de mudas de qualidade com uniformidade e idoneidade varietal surge como fator altamente relevante na implantação dos pomares de oliveiras. Dessa forma, o objetivo deste trabalho foi desenvolver protocolo de estabelecimento in vitro a partir de segmentos nodais de duas cultivares de oliveira sob diferentes concentrações de hipoclorito de sódio e concentrações de BAP. O experimento foi conduzido no delineamento inteiramente casualisado, em esquema fatorial triplo (2x3x3), sendo duas cultivares de oliveira (Arbequina e Koroneiki), três concentrações de BAP (0,00; 2,22 e 4,44 µM) e três concentrações de hipoclorito de sódio (0,6; 0,8 e 1,0% de cloro ativo) para a descontaminação dos explantes. Cada tratamento contou com três repetições e a unidade experimental constituída por cinco tubos de ensaio com um explante cada. Após 28 dias, foi possível observar que os tratamentos testados não foram eficientes no estabelecimento in vitro de Olea europaea cv. Arbequina. A concentração de 2,22 µM de BAP adicionada ao meio WPM e a concentração de 1,0% de hipoclorito de sódio, utilizada na desinfestação dos explantes, são ideais para o estabelecimento in vitro de Olea europaea cv. Koroneiki.

The objective of this work was to evaluate the influence of spectral qualities on the germination and vigor of cucurbit seeds. This experiment was a completely randomized design, in a factorial scheme 3 x 6, with three species of cucurbitaceae: cucumber, melon and watermelon. Six spectral qualities were used: blue LED, white LED, red LED, blue + red LED, fluorescent and dark (no light). Variables related to germination and vigor were analyzed. The cucurbitaceae presented differentiated responses when submitted to the different spectral qualities. For cucumber seeds, the luminous spectra increased root length, compared to the dark treatment. The melon seeds had low physiological quality; for these, the dark promoted a higher percentage of germinated and normal seeds. For watermelon seeds, the white LED conditions led to a higher percentage of normal seedlings, high rate of germination (IVG) and lower percentage of dead seeds. Red LED conditioned high IVG in watermelon. In general, fluorescent light favors carotenoid content and the dark condition favors shoot length in cucurbit seeds.

The use of highly toxic pesticides to control soil pathogens, such as Fusarium spp. and Sclerotinia sclerotiorum has generated concern, due to the irreversible impacts caused on the environment, in addition to selecting resistant isolates. In this way, essential oils appear as an efficient alternative in control of diseases. Facing the problem of soil pathogens control and high antimicrobial fungicide that essential oils present, this work aimed to evaluate the in vitro fungicidal potential of essential oils in control of Fusarium spp. and S. sclerotiorum. A completely randomized design, factorial scheme 2×4×8 was used, with two isolates (Fusarium spp. and S. sclerotiorum), four essential oils (Aloysia citriodora, Cymbopogon winterianus, Lippia alba and Ocimum americanum), eight essential oil concentrations (0.0; 0.2; 0.4; 0.6; 0.8; 1.0; 1.2 and 1.4 ?L·mL-1), and ten replicates. The essential oils inhibited mycelial growth of the fungi in different concentrations, being their potential justified by the presence of antifungal chemical compounds. Essential oils of A. citriodora, C. winterianus, L. alba and O. americanum present high fungicidal potential, being viable alternatives for formulation of commercial products, boosting the pesticides industry.

Seedling production is a critical step in the establishment of vegetables. This work aimed to evaluate the effect of different spectral qualities on the germination and vigor of endive, lettuce, and chicory seeds. The experiment was carried out in three stages. The first stage, two cultivars of lettuce (‘Crespa Repolhuda’ and ‘Vera’) and six spectral qualities (blue LED, red LED, blue + red LED, white LED, fluorescent and dark) were evaluated; the second stage, two cultivars of chicory (‘Lisa Escarola’ and ‘Palla Rosa’) and the same spectral qualities were evaluated. In the third stage, the spectral quality of the endive cultivar ‘Pão de Açúcar’ was evaluated. The experiments were performed in a completely randomized design, with four replications of 50 seeds each. The evaluated parameters were: germination percentage, first count, germination speed index, seedling length, and fresh and dry mass. Endive, lettuce and chicory seeds germinated both in the presence or not of light so that they can be classified as neutral photos. A spectral LED of red quality fostered the development of the most significant volume of fresh mass on the endive, lettuce, and chicory. All the spectral qualities stimulated root growth. The dark, on the other hand, promoted the most significant length of the aerial part, promoting seedlings etiolation.