Review of translocations detected by FISH for retrospective biological dosimetry applications

Edwards, Alan; Lindholm, Carita; Darroudi, F.; Stephan, G.; Romm, Horst; Barquinero, Joan Francesc; Barrios, Leonardo; Caballı́n, Marı́a Rosa; Roy, Laurence; Whitehouse, Caroline; Tawn, E. Janet; Moquet, Jayne; Lloyd, David; Voisin, Philippe

doi:10.1093/rpd/nch452

Cited by 95 publications

(61 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…De nombreuses publications attestent que le taux de translocations augmente significativement avec l'âge (Darroudi, 2000 ;Edwards et al, 2005 ;Pressl et al, 1999 ;Ramsey et al, 1995 ;Sigurdson et al, 2008 ;Sorokine-Durm et al, 2000a ;Whitehouse et al, 2005). Ces études peuvent être regroupées en deux groupes : celles regroupant les données issues de plusieurs laboratoires pour avoir plus de poids statistique (Sigurdson et al, 2008 ;Sorokine-Durm et al, 2000b ;Whitehouse et al, 2005), et celles qui présentent les données d'un seul laboratoire (Darroudi et Natarajan, 2000 ;Pressl et al, 1999 ;Ramsey et al, 1995) (Tab.…”

Section: Effet De L'âgeunclassified

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

et al. 2010

Self Cite

View full text Add to dashboard Cite

The assessment of exposure to ionizing radiation, carried out long time after exposure, is currently performed by scoring of translocations, a specific type of chromosomal aberrations. The translocations rate observed in peripheral blood lymphocytes of exposed subjects is compared to that observed in a control population. However, the translocation specificity towards radiation exposure is not clearly identified. To avoid any hasty conclusion, it is necessary to identify all the factors likely to induce translocation. To our knowledge, no study has thus far examined the effects of all these different factors on translocation rates. A review of the literature thus allowed us to assess the impact of host factors and lifestyle on the production of translocations. This study confirms that age appears to be the factor 1 IRSN, Laboratoire de Dosimétrie Biologique, BP 17, 92262 Fontenay-aux-Roses Cedex, France.Article publié par EDP Sciences E. GRÉGOIRE et al. 154 RADIOPROTECTION -VOL. 45 -N° 2 (2010) having the greatest impact on the rate of translocations, especially over 60 years. To date, the factor "age" is already considered in estimating the impact of radiation on the rate of translocation for all age groups. However, the study also shows that this rate varies significantly when the patient is exposed simultaneously and significantly towards many lifestyle agents. A precise threshold translocation rate should thus be established as a function of known behavioral exposures, below which it is impossible to conclude that radiological exposure has occurred. The effects of chemicals on the translocation rate after occupational exposure will be the subject of a second part.

show abstract

Section: Effet De L'âgeunclassified

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genotoxic agents, including chemicals and ionizing radiation, induce chromosomal aberrations including translocations. Because translocations frequently persist through mitosis so that the affected cells and their progeny remain viable, they are of interest as a biomarker because they allow for meaningful assessment many years after the putative exposure has occurred [5][6][7][8][9]. Increased frequencies of stable and unstable chromosome aberrations are known to occur after radiation exposure and to increase with age.…”

Section: Introductionmentioning

confidence: 99%

International study of factors affecting human chromosome translocations

Sigurdson

Hauptmann

et al. 2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

118

View full text Add to dashboard Cite

Abstract*Correspondence, page proofs, and reprint requests to: James D. Tucker, Ph.D., Department of Biological Sciences, 1370 Biological Sciences Building, 5047 Gullen Mall, Wayne State University, Detroit, TEL: (313) 577-2783, FAX: (313) 577-3602, EMAIL: E-mail: jtucker@biology.biosci.wayne.edu. z Retired Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from sixteen laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages vs. a linear relationship (p <0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p<0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes. NIH Public Access

show abstract

“…Although mtDNA mutations are recognized to accumulate with age (7,19) and mutations within the D-loop are not believed to be under strong selection pressure (18), persistence of mutations has not been tested adequately. While chromosomal translocations are a stable biomarker of radiation exposure of hematopoietic stem cells (34), the kinetics of mitochondrial replication is radically different from that of the nuclear DNA (1), and the long-term persistence of mtDNA muta- C1 100 A16235G, C16261T, C16291T, C16292T No C16021G, G16034A, C16057T, C16107T, T16292C*, G16329&T16330 del §, G16336 del, C16355T C2 96 C16069T, T16126C, G16145A, T16231C, C16261T A16302G (11) G16039A, G16047T, A16212T, C16188T, T16189C, C16348T C3 Exp7 97 G16129A, C16223T, G16391A No G16039 del, C16111T †, T16124C, G16125A, C16256T † Exp8 97 C16069T, T16126C No G16129A †, C16176T, A16258T, C16287T, G16346A † Exp9 96 T16093C, C16192T, A16207G, C16256T, C16270T, C16291T, T16324C, A16399G C16093T ( tions after historical exposure to genotoxic agents is unknown.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA Mutations in Individuals Occupationally Exposed to Ionizing Radiation

Wilding¹,

Cadwell²,

Tawn³

et al. 2006

Radiation Research

View full text Add to dashboard Cite

Mutations in a 443-bp amplicon of the hypervariable region HVR1 of the D-loop of mitochondrial DNA (mtDNA) were quantified in DNA extracted from peripheral blood samples of 10 retired radiation workers who had accumulated external radiation doses of Ͼ0.9 Sv over the course of their working life and were compared to the levels of mutations in 10 control individuals matched for age and smoking status. The mutation rate in the 10 exposed individuals was 9.92 ؋ 10 Ϫ5 mutations/ nucleotide, and for the controls it was 8.65 ؋ 10 Ϫ5 mutations/ nucleotide, with a procedural error rate of 2.65 ؋ 10 Ϫ5 mutations/nucleotide. No increase in mtDNA mutations due to radiation exposure was detectable (P ‫؍‬ 0.640). In contrast, chromosomal translocation frequencies, a validated radiobiological technique for retrospective dosimetric purposes, were significantly elevated in the exposed individuals. This suggests that mutations identified through sequencing of mtDNA in peripheral blood lymphocytes do not represent a promising genetic marker of DNA damage after low-dose or low-doserate exposures to ionizing radiation. There was an increase in singleton mutations above that attributable to procedural error in both exposed and control groups that is likely to reflect age-related somatic mutation. ᭧

show abstract

Review of translocations detected by FISH for retrospective biological dosimetry applications

Cited by 95 publications

References 34 publications

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

International study of factors affecting human chromosome translocations

Mitochondrial DNA Mutations in Individuals Occupationally Exposed to Ionizing Radiation

Contact Info

Product

Resources

About