Reversible thrombin detection by aptamer functionalized STING sensors

Actis, Paolo; Rogers, Adam; Nivala, Jeff; Vilozny, Boaz; Seger, R; Jejelowo, Olufisayo; Pourmand, Nader

doi:10.1016/j.bios.2011.05.010

Cited by 56 publications

(50 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, aptamers offer several advantages over antibodies. For instance, aptamers can easily be forced to release the bound target without harsh treatments such as high heat, salt concentrations, or pH, thus preserving the integrity of the target [84]. For example, the introduction of a sequence complementary to the aptamer sequence can be enough to compete off the binding of the target [85].…”

Section: Discussionmentioning

confidence: 99%

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Dickey

Giangrande

2016

Methods

View full text Add to dashboard Cite

A critical challenge for treating cancer is the early identification of those patients who are at greatest risk of developing metastatic disease. The number of circulating tumor cells (CTCs) in cancer patients has recently been shown to be a valuable (and non-invasively accessible) diagnostic indicator of the state of metastatic disease. CTCs are rare cancer cells found in the blood circulation of cancer patients believed to provide a means of diagnosing the likelihood for metastatic spread and assessing response to therapy in advanced, as well as early stage disease settings. Numerous technical efforts have been made to reliably detect and quantify CTCs, but the development of a universal assay has proven quite difficult. Notable challenges for developing a broadly useful CTC-based diagnostic assay are the development of easy-to-operate methods that 1) are sufficiently sensitive to reliably detect the small number of CTCs that are present in the circulation and 2) can capture the molecular heterogeneity of tumor cells. In this review, we describe recent progress towards the application of synthetic oligonucleotide aptamers as promising, novel, robust tools for the isolation and detection of CTCs. Advantages and challenges of the aptamer approach are also discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Dickey

Giangrande

2016

Methods

View full text Add to dashboard Cite

show abstract

“…On the other hand, previous researches concerning the construction of nanopore sensors were mainly focused on polymer materials based nanopores such as poly(ethylene terephthalate) (PET) [11,12,20,26,27] and polyimide (PI or Kapton) [10,14]. Only a few of these nanosensors are based on glass materials nanopore channel [13,19,28]. Compared with polymer nanopores, glass conical nanopore channel possesses several advantages include facile and inexpensive preparation, compactness, and superb mechanical robustness [16].…”

Section: Introductionmentioning

confidence: 99%

A single glass conical nanopore channel modified with 6-carboxymethyl-chitosan to study the binding of bovine serum albumin due to hydrophobic and hydrophilic interactions

Cai

Zhang

et al. 2015

Microchim Acta

View full text Add to dashboard Cite

A single glass conical nanopore functionalized with 6-carboxymethyl-chitosan (CMC) was applied to study the binding of bovine serum albumin (BSA) because of both hydrophobic and hydrophilic interactions. The interactions between the CMC-modified nanopore and BSA within the confined space were studied via the ionic current passing the nanopore by measuring the current-voltage (I-V) curves in 10 mM KCl solution. The hydrophilicity of CMC was varied by adjusting the pH values. Significant changes in the ionic current were observed following attachment of BSA. The relative contributions of hydrophobic and hydrophilic interactions depend on whether solutions are acidic or basic. A linear relationship exists between the concentration of BSA (up to 500 nM) and the ionic current at pH 12. This suggests a potential application of the method for sensing proteins via sweep voltammetry on a nanoscale. The nanodevice described here can be made reversible by ultrasonication to remove the attached BSA molecules.

show abstract

“…Thrombin binding DNA-aptamer has been extensively investigated [4]. Several types of tests have been developed to quantify thrombin, such as electrochemiluminescence, electrochemical, SPR fiber grating, end-point assays [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Thrombin detection by means of an aptamer based sensitive coating fabricated onto LMR-based optical fiber refractometer

et al. 2012

View full text Add to dashboard Cite

An optical fiber sensor using an aptamer receptor has been developed for the detection of thrombin. The sensitive coating consists of an aptamer-polymer structure fabricated using the layer-by-layer electrostatic self assembly technique onto novel optical fiber refractometers based on lossy mode resonances (LMRs). LMR based refractometers enable to overcome the traditional drawbacks of surface Plasmon resonance (SPR) based devices, such as the utilization of polarized light for example. The refractometer is based on an indium tin oxide thin layer deposited by DC-sputtering onto the cladding removed multimode fiber. These refractometers enable to monitor the variations of the surrounding medium refractive index (RI). Hence, RI variations of the outer film induced by thrombin can be referenced to the LMR wavelength shift. The described configuration has enabled the detection of low concentrations of thrombin (100nM) as well as obtaining a maximum wavelength shift of 50 nm at higher (1µM) concentrations.

show abstract

Reversible thrombin detection by aptamer functionalized STING sensors

Cited by 56 publications

References 30 publications

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

A single glass conical nanopore channel modified with 6-carboxymethyl-chitosan to study the binding of bovine serum albumin due to hydrophobic and hydrophilic interactions

Thrombin detection by means of an aptamer based sensitive coating fabricated onto LMR-based optical fiber refractometer

Contact Info

Product

Resources

About