A single glass conical nanopore channel modified with 6-carboxymethyl-chitosan to study the binding of bovine serum albumin due to hydrophobic and hydrophilic interactions

Abstract

A single glass conical nanopore functionalized with 6-carboxymethyl-chitosan (CMC) was applied to study the binding of bovine serum albumin (BSA) because of both hydrophobic and hydrophilic interactions. The interactions between the CMC-modified nanopore and BSA within the confined space were studied via the ionic current passing the nanopore by measuring the current-voltage (I-V) curves in 10 mM KCl solution. The hydrophilicity of CMC was varied by adjusting the pH values. Significant changes in the ionic current were observed following attachment of BSA. The relative contributions of hydrophobic and hydrophilic interactions depend on whether solutions are acidic or basic. A linear relationship exists between the concentration of BSA (up to 500 nM) and the ionic current at pH 12. This suggests a potential application of the method for sensing proteins via sweep voltammetry on a nanoscale. The nanodevice described here can be made reversible by ultrasonication to remove the attached BSA molecules.

One‐Step Noncovalent Surface Functionalization of PDMS with Chitosan‐Based Bioparticles and Their Protein‐Repellent Properties

“…In both the cases, no protein adsorption studies were carried out . Cai et al have studied the association (hydrophobic/hydrophilic) of bovine serum albumin (BSA) on Chi and CMChi molecules, which were covalently attached to single glass conical nanopore, by sweep voltammetry, with the aim to construct smart nanosensing devices …”

Ultraselective Monovalent Metal Ion Conduction in a Three-Dimensional Sub-1 nm Nanofluidic Device Constructed by Metal–Organic Frameworks

“…Nanofluidics have been the research hotspot in the past two decades due to their wide potential applications such as in energy harvesting and conversion, biosensing, and biomimetic ion transportation. − The advances in nanotechnology have enabled researchers to design and construct various kinds of well-defined nanofluidic systems for regulation of ion transport at nanometric scale, based on one-dimensional (1D) materials (carbon nanotubes, − glass nanopores − and polymeric nanochannels − ) and two-dimensional (2D) materials, including graphene and graphene oxide (GO), − MXenes, − h-BN, , C 3 N 4 , and other layered nanosheets . It was initially believed that the nanochannels with pore sizes comparable to hydrated diameters of ions are predicted to have enhanced ion selectivity.…”

Ultrasensitive determination of mercury(II) using glass nanopores functionalized with macrocyclic dioxotetraamines

“…Glass conical nanopore, fabricated from glass capillary, shows the characteristics of high mechanical stability, easy modification and low cost. [18,19] It exhibits excellent sensitivity in sensing of nanoparticles [20,21], small organics [22], sugars [23], proteins [24,25] and DNAs [26,27]. Herein, we present a macrocyclic dioxotetraamines derivative (C5) functionalized glass conical nanopore to achieve the ultrasensitive detection of Hg 2+ (Fig.1).…”