Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites

Mateo, César; Abián, Olga; Fernández‐Lafuente, Roberto; Guisán, José M.

doi:10.1002/(sici)1097-0290(20000405)68:1<98::aid-bit12>3.0.co;2-t

Cited by 239 publications

(162 citation statements)

References 25 publications

(24 reference statements)

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“…However, there are some drawbacks. The enzyme cannot be used under conditions where the enzyme may become desorbed from the support (pH or ionic strength will be restricted in ionic exchange [9,46,47], solvents or detergents in hydrophobic adsorptions, etc. [48].…”

Section: Immobilization Via Physical Adsorptionmentioning

confidence: 99%

“…Cantoblanco. 28049 Madrid, Spain; Tel: ----------------; Fax: --------------------; E-mail: rfl@icp.csic.es involves most of the ones that immobilize enzymes via physical adsorptions: generally a multipoint immobilization process is required to fix the enzyme to the support (e.g., ion exchangers), and that means that the support must have a high activation degree [9]. That way, the immobilized enzyme may interact with the support during storage or operation.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact on enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

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Section: Immobilization Via Physical Adsorptionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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“…Among them, carbon-based matrices are the most applied due to costs and their electronic conductivity. Aspects related with the protocols for bio-molecules immobilization on the composite support have been widely proposed for many researchers (Caramori & Fernandes, 2004;Ikeda et al, 2002;Shan et al, 2007;Mateo et al, 2000). Difficulties still pose problems such as being reproducible, simple to use, and stable immobilization of the biological component.…”

Section: Fig 1 Electrochemical Composite Biosensor For Analyte Detementioning

confidence: 99%

Graphite-Composites Alternatives for Electrochemical Biosensor

Alhadeff¹,

Bojorge²

2011

Metal, Ceramic and Polymeric Composites for Various Uses

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“…In this sense, immobilization of proteins on ionic exchangers presents several advantages because it does not involve complex protocols for enzyme immobilization; it only requires putting the enzyme solution in contact with the support under very mild conditions. The most important advantage of this method is that it is possible to reutilize the supports after enzyme inactivation (8,31,35,37,46). Therefore, ionic exchangers provide a simple solution for some immobilization problems, and in fact they were used as the first strategy to produce an industrial biocatalyst (10,24).…”

mentioning

confidence: 99%

“…The use of ionic polymers coating the surface of solid supports (e.g., polyethyleneimine [PEI] or dextran sulfate) not only provides a stronger adsorption but also prevents the alteration of the three-dimensional protein structure since the polymer becomes adapted to the enzyme instead of forcing the protein to become adapted to the support (13,31). The immobilization of proteins on this polymer-coated support may have some additional advantages, preventing subunit dissociation of multimeric enzymes (2,14,31) or generating hydrophilic nanoenvironments that stabilize the proteins against the presence of organic solvents (12). However, in some cases a protein may not be adsorbed strongly enough even on these polymeric ionic beds for a particular application.…”

mentioning

confidence: 99%

Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers

Montes

Grazú

López‐Gallego

et al. 2007

Appl Environ Microbiol

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A new mutant of the industrial enzyme penicillin G acylase (PGA) from Escherichia coli has been designed to improve its reversible immobilization on anionic exchangers (DEAE-or polyethyleneimine [PEI]-coated agarose) by assembling eight new glutamic residues distributed homogeneously through the enzyme surface via site-directed mutagenesis. The mutant PGA is produced and processed in vivo as is the native enzyme. Moreover, it has a similar specific activity to and shows the same pH activity profile as native PGA; however, its isoelectric point decreased from 6.4 to 4.3. Although the new enzyme is adsorbed on both supports, the adsorption was even stronger when supports were coated with PEI, allowing us to improve the enzyme stability in organic cosolvents. The use of restrictive conditions during the enzyme adsorption on anionic exchangers (pH 5 and high ionic strength) permitted us to still further increase the strength of adsorption and the enzyme stability in the presence of organic solvents, suggesting that these conditions allow the penetration of the enzyme inside the polymeric beds, thus becoming fully covered with the polymer. After the enzyme inactivation, it can be desorbed to reuse the support. The possibility to improve the immobilization properties on an enzyme by site-directed mutagenesis of its surface opens a promising new scenario for enzyme engineering.

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Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites

Cited by 239 publications

References 25 publications

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Graphite-Composites Alternatives for Electrochemical Biosensor

Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers

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