Reverse strand-displacement amplification strategy for rapid detection of p53 gene

Symmetric exponential amplification reaction-based DNA nanomachine for the fluorescent detection of nucleic acids

“…This scheme only involved a well-designed hairpin probe, with the advantages of ease of operation and no need for immobilization, separation or washing, and achieved comparable analytical performance to previously published methods. 11,14,18,20 More information about the comparison between the P-HP-mediated S-EXPAR assay and published methods, in terms of assay capability, is given in Table S2. †…”

“…8,9 The exponential amplication reaction (EXPAR) is an outstanding example of DNA nanomachines driven by polymerase and endonuclease, which can perform autonomous polymerization/nicking/ displacement cycles and produce a large number of sensing modules. [10][11][12][13][14][15] However, Tan et al found that further application of EXPAR was limited by a nonspecic reaction between the single-stranded template and the DNA polymerase. 16 In later studies, researchers replaced the single-stranded template with a normal or even a double-hairpin molecular beacon, to avoid this nonspecic reaction.…”

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

“…It is inspired from normal physiological RNA transcription and DNA replication, which occurs at a constant temperature. Over the past 2 decades, SDA has been widely used as an alternative to PCR for the detection of pathogens [14,15], hereditary diseases [16], and cancers [17][18][19]. Moreover, SDA amplified nucleic acids can be multiplexed and readily provide optical and visual readouts [14,20,21].…”

Ultrasensitive electrochemical platform for the p53 gene via molecular beacon-mediated circular strand displacement and terminal deoxynucleotidyl transferase-mediated signal amplification strategy

“…13,14 In recent years, electrochemical biosensors have played a signification role in the detection of DNA genes due to its specific virtues as a fast, simple, portable, and low cost tool with easy miniaturization and high sensitivity. 15 In order to achieve the highly sensitive detection of such electrochemical biosensors for the detection of target DNA genes with low abundance in actual samples, several signal amplification strategies have been widely used to enhance the detection signal, such as strand displacement reaction, 16 hybridization chain reaction, 17 loop-mediated isothermal amplification, 18 enzyme-assisted target recycling, 12,19 and rolling circle amplification. 20 Among these signal amplification approaches, the molecular beacon-mediated circular strand displacement (CSD) has attracted great attention, and is widely used to improve the specificity and sensitivity of electrochemical biosensors.…”