Symmetric exponential amplification reaction-based DNA nanomachine for the fluorescent detection of nucleic acids

Qi, Yan; Duan, Qiuyue; Huang, Yuqi; Guo, Jiang; Liu, Zhong; Wang, Hong; Ye, Gang

doi:10.1039/c9ra08854g

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…These experiments suggested that the sensor molecules behave uniformly. Interestingly, when the experiment in Figure 2 was repeated after injection of a target (p53 tumor suppressor gene: 5′-TTCCTCTGTGCGCCGGTCTCTCCT) 37,49,50 and incubated for ∼20 min, we observed a very different behavior of sensor molecules. In the presence of the target, sensor molecules showed very clear dynamics of Cy3 and Cy5 fluorescence intensities that were anticorrelated.…”

Section: ■ Results and Discussionmentioning

confidence: 95%

Single-Molecule FRET-Based Dynamic DNA Sensor

2021

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Selective and sensitive detection of nucleic acid biomarkers is of great significance in early-stage diagnosis and targeted therapy. Therefore, the development of diagnostic methods capable of detecting diseases at the molecular level in biological fluids is vital to the emerging revolution in the early diagnosis of diseases. However, the vast majority of the currently available ultrasensitive detection strategies involve either target/signal amplification or involve complex designs. Here, using a p53 tumor suppressor gene whose mutation has been implicated in more than 50% of human cancers, we show a background-free ultrasensitive detection of this gene on a simple platform. The sensor exhibits a relatively static mid-FRET state in the absence of a target that can be attributed to the time-averaged fluorescence intensity of fast transitions among multiple states, but it undergoes continuous dynamic switching between a low-and a high-FRET state in the presence of a target, allowing a high-confidence detection. In addition to its simple design, the sensor has a detection limit down to low femtomolar (fM) concentration without the need for target amplification. We also show that this sensor is highly effective in discriminating against single-nucleotide polymorphisms (SNPs). Given the generic hybridization-based detection platform, the sensing strategy developed here can be used to detect a wide range of nucleic acid sequences enabling early diagnosis of diseases and screening genetic disorders.

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Section: ■ Results and Discussionmentioning

confidence: 95%

Single-Molecule FRET-Based Dynamic DNA Sensor

2021

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“…Another advancement came from Yan et al , who constructed a novel multifunctional hairpin probe (P-HP) incorporating palindromic fragments for a symmetric EXPAR (S-EXPAR) method. 128 This design significantly reduces nonspecific amplification in the EXPAR. Similarly, Kim et al developed a target-inducible strand amplification reaction for detecting target microRNAs (miRNAs) by employing cleverly designed, interrelated reaction pathways 129 (Table 1).…”

Section: Expar-based Assays Incorporating Other Methodsmentioning

confidence: 99%