Reverse-phase High Performance Liquid Chromatography of Sennosides in Cassia angustifolia

Abstract

A rapid and simple reverse‐phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles sennoside A and sennoside B in senna. Using a Bondapak C18 column eluted with methanol:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.

An improved method for the analysis of sennosides in Cassia angustifolia by high‐performance liquid chromatography

“…The flow rate was maintained at 0.6 mL/min for the first 20 min, whilst at 30 min it was 1.0 mL/min. A calibration curve for each standard sennoside was plotted and found to be linear over the range 2-50 mg as reported earlier (Verma et al, 1996). The minimum detection limits of the method were 0.2 mg/mL for sennoside A and 0.1 mg/mL for sennoside B.…”

“…A search of the literature showed that the HPLC methods for the rapid estimation of sennosides A and B in C. angustifolia have employed a variety of extraction procedures, mobile phases and stationary phases such as C 8 (Gorler et al, 1979), C 18 (Tanaka et al, 1982;Srivastava et al, 1983;Verma et al, 1996) and Nucleosil N(CH 3 ) 2 (Ohshima and Takahashi, 1983). During our programme for the development of analytical methods for important medicinal plants, we have found that the isocratic methods reported provided inaccurate results owing to the inadequate resolution of compounds which are eluted quickly, and that the quantitative determination of sennosides A and B becomes difficult in samples which contain relatively low concentrations (2-5 mg/mL) of these two compounds.…”

“…During our programme for the development of analytical methods for important medicinal plants, we have found that the isocratic methods reported provided inaccurate results owing to the inadequate resolution of compounds which are eluted quickly, and that the quantitative determination of sennosides A and B becomes difficult in samples which contain relatively low concentrations (2-5 mg/mL) of these two compounds. Furthermore, some of the sample extraction procedures were time-consuming, and certain analyses were performed with large column diameters and with relatively high flow-rates (Ohshima and Takahashi, 1983), or at extreme pH limits of the mobile phase (Tanaka et al, 1982;Srivastava et al, 1983;Verma et al, 1996) resulting in high column backpressures during repeated analyses which could shorten the column life. This led us to develop an economically feasible HPLC method with an extraction procedure that would provide better recoveries without high column back-pressure, which would operate at moderate pH and flow-rate, and with acceptable resolution for all of the constituents present in crude extracts of C. angustifolia.…”

“…A number of isocratic HPLC methods have been reported for the analysis of sennosides (Erni and Frei, 1978;Gorler et al, 1979;Ohshima and Takahashi, 1983;Srivastava et al, 1983;Verma et al, 1996), but a baseline resolution was not achieved for all of the constituents present in crude leaf and pod extracts. This has prompted us to develop a simple, precise and reproducible method for the analysis of the compounds present in crude extracts of C. angustifolia.…”

SIMULTANEOUS DETERMINATION OF IMPORTANT ALKALOIDS IN PAPAVER SOMNIFERUM USING REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

“…Analytical reports are mainly on the simultaneous analysis of up to five alkaloids, morphine, codeine, thebaine, papaverine, and narcotine. In our efforts towards developing liquid chromatographic procedures for plant drug analysis (4)(5)(6)(7)(8), here, in this paper we are reporting a reversed-phase HPLC (RPLC) method for the simultaneous analysis of eight major alkaloids (1-8) (Fig.1) in P. somniferum samples by employing a binary mobile phase. This is the first report on the quantitation of eight alkaloids (1-8) by RPLC.…”