Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica

Verma, Rajesh; Bhartariya, K. G.; Gupta, Madan M.; Kumar, Sushil

doi:10.1002/(sici)1099-1565(199907/08)10:4<191::aid-pca456>3.0.co;2-r

Cited by 32 publications

(7 citation statements)

References 5 publications

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“…Many active compounds were extracted from C. asiatica and triterpenes were especially reported (3,16). Quantitative determination of triterpenes from C. asiatica by HPLC was previously reported (3,(16)(17)(18)(19). The retention time in this study was 8 to 17 min.…”

Section: Content Of Triterpenes In C Asiatica Extractmentioning

confidence: 99%

Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

et al. 2016

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Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-b-cyclodextrin (HP-b-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5-6.0 with viscosity in the range of 20-60 mPa s, surface tension 20.3-24.6 mN m -1 and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days.

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Section: Content Of Triterpenes In C Asiatica Extractmentioning

confidence: 99%

Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

et al. 2016

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“…The statistical analysis software was applied in this experiment to establish a regression equation (2), in this equation, the terms of Y, X 1 , X 2 , and X 3 respectively represent the yield of asiaticoside, microwave radiation time , liquid to solid ratio and enzymatic temperature:…”

Section: Quadratic Regression Orthogonal Design Resultsmentioning

confidence: 99%

“…It has been used for the treatment of hot and humid jaundice, traumatic injuries, infectious hepatitis and dermatosis [1]. Currently, some conventional extraction methods are mostly adopted for the extraction of asiaticoside [2][3][4], such as aqueous extraction and ethanol extraction.…”

mentioning

confidence: 99%

Enzymatic Pretreatment and Microwave Extraction of Asiaticoside from Centella asiatica

Fan

Han

Wang

et al. 2009

2009 3rd International Conference on Bioinformatics and Biomedical Engineering

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The extraction of asiaticoside from Centella asiatica by enzymatic pretreatment and microwave extraction (EPME) was studied in this article. The effects of several important factors such as temperature of enzymatic pretreatment, liquid to solid ratio and microwave radiation time were investigated by orthogonal central composite design experiment. An extraction model with well forecast performance was established. The results indicate that the optimum extraction condition was as follows: liquid to solid ratio was 36 mL/g, temperature of enzymatic pretreatment was 45 ℃, enzymatic time was 30 min, and microwave radiation time was 110 s. On such conditions the yield was 27.10%.

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“…Analytical reports are mainly on the simultaneous analysis of up to five alkaloids, morphine, codeine, thebaine, papaverine, and narcotine. In our efforts towards developing liquid chromatographic procedures for plant drug analysis (4)(5)(6)(7)(8), here, in this paper we are reporting a reversed-phase HPLC (RPLC) method for the simultaneous analysis of eight major alkaloids (1-8) (Fig.1) in P. somniferum samples by employing a binary mobile phase. This is the first report on the quantitation of eight alkaloids (1-8) by RPLC.…”

Section: Simultaneous Determination Of Important Alkaloids In Papavermentioning

confidence: 99%

SIMULTANEOUS DETERMINATION OF IMPORTANT ALKALOIDS IN PAPAVER SOMNIFERUM USING REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Singh

Prajapati

Bajpai

et al. 2000

Journal of Liquid Chromatography & Related Technologies

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Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica

Cited by 32 publications

References 5 publications

Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

Enzymatic Pretreatment and Microwave Extraction of Asiaticoside from Centella asiatica

SIMULTANEOUS DETERMINATION OF IMPORTANT ALKALOIDS IN PAPAVER SOMNIFERUM USING REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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