Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

Abstract

Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-b-cyclodextrin (HP-b-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5-6.0 with viscosity in the range of 20-60 mPa s, surface tension 20.3-24.6 mN m -1 and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days.

Wound Healing Activity of the Essential Oil of Bursera morelensis, in Mice

“…The healing process depends on the biosynthesis and deposition of collagen and its maturation [36]. Our results showed that both EO treatments had the better structure of scar tissue and a greater deposit of collagen.…”

Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review

“…The C. asiatica extract and its triterpenoids had certain therapeutic and relieving effects on acne, baldness, vitiligo, atopic dermatitis, and wounds (Sawatdee et al, 2016;Choi et al, 2017;Ling et al, 2017;Ju Ho et al, 2018;Shen et al, 2019) (Table 4).…”

Natural actives for wound healing: A review

“…Several studies demonstrated the effect of C. asiatica in wound healing when topically applied (Gohil et al, ; Gul Satar et al, ; Hong et al, ; Sawatdee, Choochuay, Chanthorn, & Srichana, ). Its mechanism of action has been attributed to increase cellular proliferation and collagen synthesis at the wound site (Pazyar et al, ; Sunilkumar, Parameshwaraiah, & Shivakumar, ), increase in angiogenesis (Incandela et al, ; Rosen, Blumenthal, & McCallum, ), and it has an effect on keratinization, which aids in thickening skin in areas of infection (Poizot & Dumez, ).…”

“…Commercial enzymatic debriding agents are available, and some previous studies reported their efficacy mainly in the treatment of burn wounds (Cordts et al, 2016;Rosenberg et al, 2014;Schulz et al, 2017) and to clear bruises and hematomas on boxers (Blonstein, Bromelain (Dutta & Bhattacharyya, 2013;Ali et al, 2015;Praveen et al, 2014;Manosroi et al, 2014;Ataide, de Carvalho, et al, 2017;Ekambaram et al, 2017) (Dutta & Bhattacharyya, 2013;Hu et al, 2011;Singer et al, 2010;Wu et al, 2012) (Blonstein, 1960;Cordts et al, 2016;Koller et al, 2008;Krieger et al, 2012;Rosenberg et al, 2004;Rosenberg et al, 2014;Schulz et al, 2017) Propolis ( Centella asiatica (Hong et al, 2005;Shukla, Rasik, Jain, et al, 1999;Gohil et al, 2010;Incandela et al, 2001;Zhang et al, 2016) (Shukla, Rasik, Jain, et al, 1999;Sawatdee et al, 2016;Gohil et al, 2010;Gul Satar et al, 2013;Sunilkumar et al, 1998;Rosen et al, 1967;Incandela et al, 2001;Poizot & Dumez, 1978;Suguna et al, 1996;Zhang et al, 2016) …”