An improved method for the analysis of sennosides in<i> Cassia angustifolia</i> by high‐performance liquid chromatography

Bala, Shashi; Uniyal, G. C.; Dubey, Toshi; Singh, Satnam

doi:10.1002/pca.586

Cited by 21 publications

(9 citation statements)

References 9 publications

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“…Many studies have reported the quantitative analysis of SA and SB by isocratic HPLC [2,3], gradient HPLC [4], ion-pair HPLC [5][6][7], and capillary electrophoresis [8,9]. Some of these studies used solid-phase extraction (SPE) to remove the interfering components.…”

Section: Introductionmentioning

confidence: 99%

Simple and rapid analysis of sennoside A and sennoside B contained in crude drugs and crude drug products by solid-phase extraction and high-performance liquid chromatography

et al. 2009

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The sennoside A (SA) and sennoside B (SB) contents of various samples of crude drugs were determined using solid-phase extraction (SPE) and HPLC. The samples examined were crude drugs (senna leaf, senna pods, and rhubarb), conventional crude drug products, and Kampo formulations. The sample solution was purified using an Oasis MAX cartridge, which has strong anion-exchange and reversed-phase properties. The samples containing SA and SB were dissolved in a solution of methanol-0.2% sodium bicarbonate (7:3, v/v) and applied to the Oasis MAX cartridge. The cartridge was washed with a solution of methanol containing 1% acetic acid. SA and SB were eluted with methanol-water-formic acid (70:30:2, v/v), and the eluate was used as the sample solution for HPLC analysis. SA and SB were analyzed using a conventional octadecylsilyl (ODS) column at a detection wavelength of 380 nm; water-acetonitrile-phosphoric acid (800:200:1, v/v) was used as the mobile phase. The SA and SB components in most samples were completely separated from other interfering constituents within 10 min. In particular, several interfering peaks adjacent to the SB peak were eliminated by SPE using the Oasis MAX cartridge. On subjecting the Kampo extracts to an additional recovery experiment, high recovery rates of SA and SB were obtained. The method employed in this study proved to be a simple and rapid method for the quantification of SA and SB.

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Section: Introductionmentioning

confidence: 99%

Simple and rapid analysis of sennoside A and sennoside B contained in crude drugs and crude drug products by solid-phase extraction and high-performance liquid chromatography

et al. 2009

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“…HPLC analysis for the estimation of sennoside A and B was performed following the protocol modified from Bala et al (2001). The solvent system consisted of solvent A: methanol + water + acetic acid (20:80:0.1) and solvent B: methanol + water + acetic acid (80:20:0.1).…”

Section: Phytochemical Analysismentioning

confidence: 99%

Micropropagation and validation of genetic and biochemical fidelity amongst regenerants of Cassia angustifolia Vahl employing RAPD marker and HPLC

Chetri

Sardar

Agrawal

2014

Physiol Mol Biol Plants

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In vitro protocol has been established for clonal propagation of Cassia angustifolia Vahl which is an important source of anticancerous bioactive compounds, sennoside A and B. Nodal explants excised from field raised elite plant (showing optimum level of sennoside A and B) of C. angustifolia when reared on Murashige and Skoog's medium augmented with different cytokinins, viz. N(6)-benzyladenine (BA), N(6)-(2-isopentenyl) adenine (2iP) and 6-furfuryl aminopurine (Kn) differentiated multiple shoots in their axils. Of the three cytokinins, BA at 5 μM proved optimum for differentiating multiple shoots in 95 % cultures with an average of 9.14 shoots per explant within 8 weeks of culture. Nearly, 95 % of the excised in vitro shoots rooted on half strength MS medium supplemented with 10 μM indole-3-butyric acid (IBA). The phenotypically similar micropropagated plants were evaluated for their genetic fidelity employing random amplified polymorphic DNA (RAPD) markers. Eleven individuals, randomly chosen amongst a population of 120 regenerants were compared with the donor plant. A total of 36 scorable bands, ranging in size from 100 to 1,000 bp were generated amongst them by the RAPD primers. All banding profiles from micropropagated plants were monomorphic and similar to those of mother plant proving their true to the type nature. Besides, high performance liquid chromatography evaluation of the sennoside A and B content amongst leaves of the mature regenerants and the elite mother plant too revealed consistency in their content.

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“…Other procedures for the detection of sennosides include reversed -phase column liquid chromatography. 33 Metabolites of anthranoid compounds in senna produce discoloration of the urine that can cause false -positive tests for urinary urobilinogen.…”

Section: Diagnostic Testingmentioning

confidence: 99%

Senna ( Senna alexandrina P. Mill.)

Barceloux

2008

Medical Toxicology of Natural Substances

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An improved method for the analysis of sennosides in Cassia angustifolia by high‐performance liquid chromatography

Cited by 21 publications

References 9 publications

Simple and rapid analysis of sennoside A and sennoside B contained in crude drugs and crude drug products by solid-phase extraction and high-performance liquid chromatography

Simple and rapid analysis of sennoside A and sennoside B contained in crude drugs and crude drug products by solid-phase extraction and high-performance liquid chromatography

Micropropagation and validation of genetic and biochemical fidelity amongst regenerants of Cassia angustifolia Vahl employing RAPD marker and HPLC

Senna ( Senna alexandrina P. Mill.)

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