Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA

Streitner, Nadine; Voss, Carsten; Flaschel, Erwin

doi:10.1016/j.jbiotec.2007.06.011

Cited by 38 publications

(26 citation statements)

References 20 publications

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“…It has been receiving a sustained attention in the medicine, food projects and biological engineering. Compared with the conventional purification methods, the extraction via reversed micellar system has the advantages of less operating procedures, more facility in manipulation, lower cost, higher specificity and capacity for preventing enzyme denaturalization [3,[17][18][19][20][21][22].…”

Section: Fundamentals Of Enzyme-containing Reversed Micellar Systemmentioning

confidence: 99%

Effects of surfactants on enzyme-containing reversed micellar system

et al. 2011

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With the development of colloid interface and enzyme technologies, enzyme-containing reversed micellar system has been receiving much attention in bioseparation and bioconversion. Because of its high efficiency, it has brought new opportunities for the development of molecular biotechnology. Reversed micelles represent nano-sized aqueous droplets stabilized by surfactant amphiphiles inside the bulk organic solvents. The entrapped enzymes have enhanced activities under those conditions as suited in the lipid bilayers of biological membranes. The fundamentals of enzyme-containing reversed micellar system are described in this paper, with special emphasis on the effects of surfactants varying in concentrations and structures. The latest study progress on the surfactants application in enzyme-containing reversed micelles is reviewed. The introduction of novel functional surfactants in micellar enzymology and their future development are also discussed. reversed micelle, enzyme, surfactant concentration, surfactant molecular structure, enzyme-containing reversed micellar system.

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Section: Fundamentals Of Enzyme-containing Reversed Micellar Systemmentioning

confidence: 99%

Effects of surfactants on enzyme-containing reversed micellar system

et al. 2011

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“…Amongst the nonionic/acetonitrile systems, the binodal curves of the Triton X-100/acetonitrile systems were closer to the origin than the binodal curves of Tween80/acetonitrile systems. This phenomenon was attributed to Tween's higher hydrophobicity [35]. Higher hydrophobicity causes poor acetonitrile solubility and miscibility; thus, a lower concentration of phase components (surfactant and acetonitrile) is needed to form the two-phase system.…”

Section: Phase Diagram Of Surfactants and Acetonitrilementioning

confidence: 99%

A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus

Amid

Manap

Asmadi

et al. 2015

Journal of Chromatography B

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a b s t r a c tPolygalacturonase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. A novel liquid/liquid extraction process composed of surfactant and acetonitrile was employed for the first time to purify polygalacturonase from Durio zibethinus. The influences of different parameters such as type and concentration of surfactants, concentrations of acetonitrile and composition of surfactant/acetonitrile on partitioning behavior and recovery of polygalacturonase was investigated. Moreover, the effect of pH of system and crude load on purification fold and yield of purified polygalacturonase were studied. The results of the experiment indicated the polygalacturonase was partitioned into surfactant top rich phase with impurities being partitioned into acetonitrile bottom rich phase in the novel method of liquid/liquid process composed of 23% (w/w) Triton X-100 and 19% (w/w) acetonitrile, at 55.6% of TLL (tie line length) crude load of 25% (w/w) at pH 6.0. Recovery and recycling of components also was measured in each successive step of liquid/liquid extraction process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 97.3% while phase components were also recovered and recycled above 95%. This study demonstrated that the novel method of liquid/liquid extraction process can be used as an efficient and economical extraction method rather than the traditional methods of extraction for the purification and recovery of the valuable enzyme.

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“…Die Aufarbeitung der Nukleinsäuren erfolgte nach Lemmens et al [9], mit geringfü-gigen Modifikationen [25]. Die dort beschriebene Anionenaustauschchromatographie mittels Fractogel-DEAE wurde auch zur weiteren Aufreinigung wässriger Rezeptorphasen verwendet, die aus der Rückextraktion mit inversmizellaren Zweiphasensystemen stammten.…”

Section: Plasmid-dna-aufarbeitungunclassified

Isolierung von Plasmid‐DNA durch inversmizellare Zweiphasensysteme – Optimierung der Rückextraktion

Streitner

Voss

Flaschel

2008

Chemie Ingenieur Technik

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Die Verteilung von RNA und Plasmid-DNA wurde in einem inversmizellaren Zweiphasensystem bestehend aus Trioctylmethylammoniumchlorid (TOMAC) und Isooctan untersucht.Die Auswirkung verschiedener Salze und Alkohole auf den Transfer der Nukleinsäuren wurde vor allem unter dem Gesichtspunkt einer hohen Wiederfindung der Plasmid-DNA nach der Rückextraktion betrachtet. Systeme, die eine Trennung von Plasmid-DNA und RNA ermöglichen, wurden eingesetzt, um RNA aus bakteriellem Klarlysat zu entfernen.

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Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA

Cited by 38 publications

References 20 publications

Effects of surfactants on enzyme-containing reversed micellar system

Effects of surfactants on enzyme-containing reversed micellar system

A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus

Isolierung von Plasmid‐DNA durch inversmizellare Zweiphasensysteme – Optimierung der Rückextraktion

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