A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus

Amid, Mehrnoush; Manap, Yazid Abdul; Asmadi, Farhana Azmira; Hussin, Muhaini; Sarker, Zaidul Islam

doi:10.1016/j.jchromb.2015.04.034

Cited by 8 publications

(3 citation statements)

References 39 publications

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“…The photographs were observed for morphological characteristics and to confirm the spherical nature of the microspheres. To determine the sphericity, the tracings of lipids microspheres (magnification 45 X) were taken on a black paper using Camera Lucida, (Model-Prism type, Rolex, India), and circulatory factor was calculated 14 . The sphericity of microspheres was calculated using the equation, S= p 2 / (12.56 × A)…”

Section: Yield Of Microspheresmentioning

confidence: 99%

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2022

IJPSR

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Microspheres drug delivery system have improved patient compliance, decreased toxicity, and increased efficacy. This work aimed to design a controlled-release drug-delivery system for lipid microspheres of Verapamil hydrochloride that would retain the drug in the stomach and continuously release the drug in a controlled manner up to a predetermined time. Lipid microspheres were prepared by using a melt solidification method. Microspheres with spherical shapes and narrow size distribution were formed. In the present investigation, two lipids were used in various concentrations; Ceresine wax & Bees Wax. In-vitro performance was evaluated, such as particle size analysis, drug entrapment efficiency, compatibility study, Preformulation study, flow properties, in-vitro drug release studies, stability studies, etc. The drug in microspheres was found to be stable and compatible with waxes, as confirmed by DSC and FTIR methods. The mixing ratio of lipids with drug affected the size, size distribution, Yield, drug content, and drug release of microspheres. Results showed that the solid, discrete, reproducible free-flowing microspheres were obtained. In most cases, good in vitro microsphere behavior was observed, and a broad variety of drug release patterns could be achieved by variation of the drug-lipid ratio. The release kinetics followed different transport mechanisms. The surface morphology of microspheres was characterized by scanning electron microscopy (SEM). The release of the drug was controlled for more than 12h. INTRODUCTION:The need to design new drug delivery systems will reduce or eliminate the variable plasma concentration. Controlled-release drug delivery systems are developed to address many of the difficulties associated with traditional methods of administration 1 . The real issue in developing oral controlled release dosage forms is to prolong the residence time of the dosage form in the stomach or upper gastrointestinal tract until the drug is completely released 2 .

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Section: Yield Of Microspheresmentioning

confidence: 99%

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2022

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“…Extraction of crude enzyme polygalacturonase (PG) was conducted according to the methods of Terefe et al (2009) and Amid et al (2015). First, durian samples and 1.2M NaCl solution, pH 6.0 (1:6 w/v) were mixed thoroughly for 30 mins in ice.…”

Section: Crude Enzyme Extractionmentioning

confidence: 99%

Shelf life determination of durian (Durio zibethinus) paste and pulp upon highpressure processing

Tan¹,

Ng²,

Tan³

et al. 2018

Food Res.

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Globally, there has been an increase in demand for durian. Therefore, it is important to extend the shelf life and at the same time, maintain the quality of durian. This study investigated the effect of high-pressure processing (HPP) on the shelf life of durian paste and pulp. Specifically, HPP treatments of 500 MPa for 5 min on nylon-and skin filmpacked durian pulp, and 600 MPa for 5 min on nylon-packed durian paste were applied. It was found that throughout the 56-day storage period, the total soluble solids (TSS), pH and titratable acidity of durian paste and pulp showed no significant changes (p>0.05). The colour (L* values) of nylon-packed untreated durian pulp showed no significant changes (p>0.05), while the untreated durian paste and skin film-packed durian pulp showed significant changes (p<0.05). As for chroma values, it showed no significant changes (p>0.05) throughout the storage period. The microbial levels of all the highpressure (HP)-treated durian samples remained below the detection limit till the end of the storage study. HP-treated durian samples showed lower enzymatic (polygalacturonase and pectin methyl esterase) activities compared to untreated sample throughout the storage period. Sensory evaluation showed no significant difference (p>0.05) between untreated and HP-treated samples. In summary, HPP effectively maintained the overall quality of durian for a minimum of 56 days.

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“…Another important requirement for droplet microfluidics-based LLE is the ability to investigate the effect of coexisting substances on the extraction process. , While such an investigation is essential for developing efficient additives that act as extraction agents, it is also highly difficult to perform because the size of the generated droplets is also affected when the compositions of the continuous and dispersed phases are altered. If droplets of a constant size are generated and LLE is initiated by exchanging the carrier fluid of the droplets (continuous phase) with another fluid containing the target and coexisting molecules, it is possible to eliminate the influence of droplet-size variations.…”

mentioning

confidence: 99%

Microfluidic System Enabling Multistep Tuning of Extraction Time Periods for Kinetic Analysis of Droplet-Based Liquid–Liquid Extraction

et al. 2016

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When analyzing the kinetics of liquid-liquid extraction (LLE), the change in the concentration of extracted target molecules over time should be monitored for a known interfacial area. Herein, we developed a microfluidic system for precisely analyzing the kinetics of LLE using droplets of a constant size even in the presence of additives. Extraction is initiated by exchanging the carrier fluid for the droplets with a target solution and then terminated by phase separation, based on the principle of hydrodynamic filtration. By using one out of several pairs of outlet/buffer inlet at a given time, the extraction time period is tuned stepwise without changing the flow rate condition. We successfully demonstrated droplet-based LLE by controlling the extraction period from ∼0.03 to ∼1.2 s and evaluated the extraction kinetics of rhodamine B from the continuous aqueous phase to droplets of 1-octanol with a diameter of ∼40 μm. In addition, the effect of additives on extraction efficiency was evaluated. The system presented in this study would be useful for determining rate constants for interfacial mass transfer in general LLE kinetic studies as well as for developing new extraction chemistries and optimizing microfluidic chemical/biochemical analysis systems that involve an LLE process.

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A novel liquid/liquid extraction process composed of surfactant and acetonitrile for purification of polygalacturonase enzyme from Durio zibethinus

Cited by 8 publications

References 39 publications

Untitled

Untitled

Shelf life determination of durian (Durio zibethinus) paste and pulp upon highpressure processing

Microfluidic System Enabling Multistep Tuning of Extraction Time Periods for Kinetic Analysis of Droplet-Based Liquid–Liquid Extraction

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