Effects of surfactants on enzyme-containing reversed micellar system

Liang, Yue; Zeng, Guangming; Zhong, Hua; Li, Hui; Wang, Weiwei

doi:10.1007/s11426-011-4266-2

Cited by 15 publications

(9 citation statements)

References 51 publications

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“…This approach could provide an opportunity to diversify the portfolio and pipelines for the treatment options considered for COVID-19 or other serious infections. There are numerous other applications in medicine, especially for protein extraction and bioactive molecule separation and purification, as well as enzyme drug delivery [45].…”

Section: Enzymes and Biocatalystsmentioning

confidence: 99%

Biosurfactants’ Potential Role in Combating COVID-19 and Similar Future Microbial Threats

et al. 2020

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During 2020, the world has experienced extreme vulnerability in the face of a disease outbreak. The coronavirus disease 2019 (COVID-19) pandemic discovered in China and rapidly spread across the globe, infecting millions, causing hundreds of thousands of deaths, and severe downturns in the economies of countries worldwide. Biosurfactants can play a significant role in the prevention, control and treatment of diseases caused by these pathogenic agents through various therapeutic, pharmaceutical, environmental and hygiene approaches. Biosurfactants have the potential to inhibit microbial species with virulent intrinsic characteristics capable of developing diseases with high morbidity and mortality, as well as interrupting their spread through environmental and hygiene interventions. This is possible due to their antimicrobial activity, ability to interact with cells forming micelles and to interact with the immune system, and compatibility with relevant processes such as nanoparticle synthesis. They, therefore, can be applied in developing innovative and more effective pharmaceutical, therapeutics, sustainable and friendly environmental management approaches, less toxic formulations, and more efficient cleaning agents. These approaches can be easily integrated into relevant product development pipelines and implemented as measures for combating and managing pandemics. This review examines the potential approaches of biosurfactants as useful molecules in fighting microbial pathogens both known and previously unknown, such as COVID-19.

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Section: Enzymes and Biocatalystsmentioning

confidence: 99%

Biosurfactants’ Potential Role in Combating COVID-19 and Similar Future Microbial Threats

et al. 2020

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“…The hydrolysis of protein components by neutral proteases reduced the degree of crosslinking, making cellulose and starch in DRB more susceptible to combine cellulase and α-amylase to enhance the effect of saccharification. In addition, protease can act as a surfactant, which promotes the enzymatic hydrolysis of glucoamylase (Liang et al 2011).…”

Section: Hydrolytic Effect Of Neutral Protease On the Cellulase And α-Amylasementioning

confidence: 99%

Simultaneous hydrolysis and fermentation of defatted rice bran and defatted soybean meal for nisin production with engineered Lactococcus lactis

Liu

et al. 2020

BioRes

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This work aimed to study the potential of defatted rice bran (DRB) and defatted soybean meal (DSM) as carbon and nitrogen sources for Lactococcus lactis growth and nisin production. First, a maximum nisin yield of 3630 IU/mL was achieved using 40% DRB hydrolysates and 30% DSM hydrolysates, which was 1.13 times greater than that found in commercial media. Second, to simplify the operation and shorten the length of the entire process, the processes of combined hydrolysis of DRB-DSM followed by fermentation, and simultaneous hydrolysis and fermentation of DRB-DSM were developed. Neutral proteinase enhanced the saccharification of DRB by cellulase and α-amylase. Furthermore, the strategy of NADH oxidase expression and hemin addition was innovatively proposed to overcome the oxygen stress in a simultaneous hydrolysis and fermentation process, which could alleviate the lag period following inoculation of L. lactis and result in a 77.3% increase in nisin titer.

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“…Enzyme-contained in a reverse micellar system is also a feasible strategy for enzyme stabilization [93]. The reversed micelle is formed by surfactant amphiphiles self-aggregated in the bulk apolar organic solvents.…”

Section: Polymers and Surfactantsmentioning

confidence: 99%

“…Because of the formed polar cores, reversed micellar system allows nanometer-sized aqueous droplets stabilized in it. The process of enzyme solubilization in reversed micelles results in the formation of enzyme-containing reversed micellar system, and the entrapped enzymes have enhanced activities under those conditions as suited in the lipid bilayers of biological membranes [93]. The surfactant concentration influences greatly the enzyme solubilization and its catalytic efficiency.…”

Section: Polymers and Surfactantsmentioning

confidence: 99%

Practical insights on enzyme stabilization

Silva

Martins

et al. 2017

Critical Reviews in Biotechnology

174

113

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Enzymes are efficient catalysts designed by nature to work in physiological environments of living systems. The best operational conditions to access and convert substrates at the industrial level are different from nature and normally extreme. Strategies to isolate enzymes from extremophiles can redefine new operational conditions, however not always solving all industrial requirements. The stability of enzymes is therefore a key issue on the implementation of the catalysts in industrial processes which require the use of extreme environments that can undergo enzyme instability. Strategies for enzyme stabilization have been exhaustively reviewed, however they lack a practical approach. This review intends to compile and describe the most used approaches for enzyme stabilization highlighting case studies in a practical point of view.

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Effects of surfactants on enzyme-containing reversed micellar system

Cited by 15 publications

References 51 publications

Biosurfactants’ Potential Role in Combating COVID-19 and Similar Future Microbial Threats

Biosurfactants’ Potential Role in Combating COVID-19 and Similar Future Microbial Threats

Simultaneous hydrolysis and fermentation of defatted rice bran and defatted soybean meal for nisin production with engineered Lactococcus lactis

Practical insights on enzyme stabilization

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