Reversal of ultraviolet B‐induced immunosuppression by inhibition of the extracellular signal‐regulated mitogen‐activated protein kinase

Liang, Hong; Luo, Xiaoqun; Ma, Li; Kang, Kefei; Zhang, Zheng

doi:10.1111/j.1600-0781.2009.00458.x

Cited by 7 publications

(4 citation statements)

References 44 publications

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“…In addition, mitogen-activated protein kinases (MAPKs) regulate key proinflammatory pathways following stimulation with UV and TNF-α (4,5). Three MAPK proteins, i.e., extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 MAPK are thought to play different roles in chronic inflammatory diseases and homeostasis in the skin (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Down-regulation of IL-6, IL-8, TNF-α and IL-1β by glucosamine in HaCaT cells, but not in the presence of TNF-α

Park

Lee²,

Cho³

et al. 2010

Oncology Letters

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Abstract. There is considerable evidence that glucosamine exerts an inhibitory effect on inflammatory cytokine expression in cells. Glucosamine has been recommended as a promising anti-inflammatory modulator, which has been applied in clinical trials for attenuation of the inflammatory process. However, it is unknown whether glucosamine reduces the expression of TNF-α-induced inflammatory cytokines in HaCaT cells. The anti-inflammatory effects of curcumin in HaCaT cells have been extensively investigated in several studies. Thus, in this study we investigated the expression of IL-6, IL-8, TNF-α and IL-1β in glucosamine-treated HaCaT cells, and the effects of glucosamine were compared to those of curcumin-treated HaCaT cells. Our data showed that the expression of IL-6, IL-8, TNF-α and IL-1β was decreased by glucosamine treatment in the HaCaT cells. In contrast, the expression of IL-6, IL-8, TNF-α and IL-1β was not attenuated by glucosamine treatment in the TNF-α-treated HaCaT cells. Notably, curcumin induced an increased expression of IL-8 and IL-1β in the HaCaT cells, but not that of IL-6 and TNF-α. On the other hand, curcumin attenuated the expression of IL-6 and IL-8 in the TNF-α-treated HaCaT cells. Our data indicated that glucosamine induced the down-regulation of IL-6, IL-8, TNF-α and IL-1β expression in the HaCaT cells. However, the stimulation of TNF-α abolished the inhibitory effects of glucosamine on the expression of inflammatory cytokines in the HaCaT cells. Thus, even though glucosamine induces the down-regulation of inflammatory cytokines in HaCaT cells, the anti-inflammatory role of glucosamine in TNF-α-mediated inflammatory skin diseases should be investigated.

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Section: Introductionmentioning

confidence: 99%

Down-regulation of IL-6, IL-8, TNF-α and IL-1β by glucosamine in HaCaT cells, but not in the presence of TNF-α

Park

Lee²,

Cho³

et al. 2010

Oncology Letters

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“…MAPK regulate important inflammation pathways following stimulation with UVB irradiation (Carlsen et al, 2004 ; Hong et al, 2009 ; Muthusamy and Piva, 2010 ). UVB radiation exposure can lead to the generation of ROS, which activate various cellular signaling pathways, including the MAPK signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

Molecular mechanism of the anti-inflammatory and skin protective effects of Syzygium formosum in human skin keratinocytes

Lee,

Choi

et al. 2023

Food Sci Biotechnol

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Irradiation injury, especially caused by UVB, of the skin is one of the critical reasons for skin inflammation and damage. The present study aimed to explore the protective effect of Syzygium formosum leafy extract (SFLE) and its mechanism of action against UVB-induced damages of human keratinocytes. In this study, SFLE was prepared from 100 kg dried leaves using industrial-scale processes. We found that SFLE markedly reduced markers of the skin inflammation in UVB-induced pro-inflammatory cytokines. Only 2 μg/mL of SFLE exhibited significantly stronger anti-inflammatory effects than the fivefold concentration of positive control. Intriguingly, an anti-inflammatory enzyme, heme oxygenase-1 expression was significantly induced by SFLE treatment. MMP-3 and -9 were, but not MMP-1, significantly reduced. SFLE inhibited the expression of the MAPK pathway, resulting in a decrease on UVB-induced reactive oxygen species. In conclusion, SFLE can potentially be used to treat skin inflammatory diseases.

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“…Considering the side effects reported in the clinical trials, topical application of MEK inhibitors is an ideal treatment. So far, MEK inhibitors have been used topically only in mouse models (Jaffee et al, 2000;Leng et al, 2009). We hope safe and effective topical MEK inhibitors for human skin diseases will be developed in the near future.…”

Section: Discussionmentioning

confidence: 99%

Blocking MAPK Signaling Downregulates CCL21 in Lymphatic Endothelial Cells and Impairs Contact Hypersensitivity Responses

Miyagaki

Sugaya

Okochi

et al. 2011

Journal of Investigative Dermatology

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CCL21 expression by lymphatic endothelial cells (LECs) is essential for migration of CCR7+ immune cells from skin to regional lymph nodes (LNs). We investigated the importance of mitogen-activated protein kinase (MAPK) signaling in CCL21 expression by ECs in vitro and in vivo. Normal human dermal lymphatic microvascular ECs (HMVEC-dLy) stimulated in vitro with oncostatin M (OSM) expressed high amounts of CCL21 mRNA. CCL21 protein expression by HMVEC-dLy was also markedly increased by OSM compared with unstimulated cultures. Marked phosphorylation of MAPK 44/42 was detected in HMVEC-dLy stimulated by OSM. CCL21 expression by HMVEC-dLy was blocked by a JAK inhibitor 1, JAK3 inhibitor, and U0126 (a MAPK kinase inhibitor) in vitro, all of which blocked phosphorylation of MAPK 44/42. In addition, injection of U0126 into murine skin significantly decreased CCL21 mRNA and protein expression. Moreover, injection of U0126 before sensitization decreased migration of dendritic cells to draining LNs and decreased contact hypersensitivity responses. In summary, these results suggest that the MAPK pathway is important for CCL21 expression by LECs in vitro and in vivo. Blocking MAPK signaling within skin may offer a novel approach to treatment of inflammatory skin diseases.

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Reversal of ultraviolet B‐induced immunosuppression by inhibition of the extracellular signal‐regulated mitogen‐activated protein kinase

Abstract: The ERK1/2 mitogen-activated protein kinase (MAPK) pathway plays an important role in the local UVB-induced immunosuppression, and its specific inhibitor PD can arrest its function, resulting in protection against UVB-induced immunosuppression in the present in vivo study.

Cited by 7 publications

References 44 publications

Down-regulation of IL-6, IL-8, TNF-α and IL-1β by glucosamine in HaCaT cells, but not in the presence of TNF-α

Down-regulation of IL-6, IL-8, TNF-α and IL-1β by glucosamine in HaCaT cells, but not in the presence of TNF-α

Molecular mechanism of the anti-inflammatory and skin protective effects of Syzygium formosum in human skin keratinocytes

Blocking MAPK Signaling Downregulates CCL21 in Lymphatic Endothelial Cells and Impairs Contact Hypersensitivity Responses

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