Revealing the mode of action of DNA topoisomerase I and its inhibitors by atomic force microscopy

Argaman, Miriam; Bendetz-Nezer, Sarit; Matlis, Sophie; Segal, Shraga; Priel, Esther

doi:10.1016/s0006-291x(03)00025-1

Cited by 8 publications

(9 citation statements)

References 40 publications

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“…A comparison of AFM results with that of TOPO I crystal structure (refer to Table I), revealed that the measured heights by AFM are much smaller than the predicted value from the crystal structure of TOPO I, whereas the lengths and widths are much bigger than those of crystal structure of TOPO I. These results are similar to those of calf thymus TOPO I in previous AFM studies (Argaman et al 2003). We speculated that the TOPO I structure may be transformed during the adsorption process on mica surface.…”

Section: Resultssupporting

confidence: 81%

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Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy

Liu

Meng

et al. 2009

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In this study, the topography of human topoisomerase I (TOPO I) on mica surfaces in air and in liquid has been studied by atomic force microscopy (AFM). The average height of TOPO I on mica surface in air measured by AFM was 2.59+/-0.32 nm. After adsorption of the 0.3 U/microl TOPO I on mica surfaces for 2 h, and then imaged in liquid by AFM, well-separated single TOPO I was observed. The average height of TOPO I on mica surfaces in liquid measured by AFM was 2.93+/-0.42 nm. After adsorption of the 4 U/microl TOPO I on mica surfaces for 1.5 h, TOPO I monolayer can be formed. The produced TOPO I monolayer on mica was flat and exhibited good stability.

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Section: Resultssupporting

confidence: 81%

“…More recently, calf thymus TOPO I has been studied by AFM in air (Argaman et al 2003). To our knowledge, structural study of human TOPO I on a solid surface in air or in liquid has not been addressed.…”

Section: Introductionmentioning

confidence: 99%

Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy

Liu

Meng

et al. 2009

Scanning

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“…41 Mammalian topoisomerase I is sensitive to camptothecin analogues, and its inhibition plays a significant role in cancer chemotherapy. 165,169 Mammalian topoisomerase III is as yet not involved in chemotherapy. 170 Antibiotic activity concentrations of a wide variety of quinolones parallel those that inhibit the enzymes.…”

Section: Molecular Mode Of Actionmentioning

confidence: 99%

Bacterial Topoisomerase Inhibitors: Quinolone and Pyridone Antibacterial Agents

2005

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“…AFM provides direct visualization of DNA-protein complexes with nanometre resolution. 21,[23][24][25] Three dimensional AFM plots of 300 bp, 535 bp and 936 bp cDNA in the presence and absence of hTopo I are shown in Fig. 3 and Fig.…”

Section: Communicationmentioning

confidence: 99%