Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy

Liu, Zhiguo; Meng, Ronghua; Zu, Yuangang; Li, Qingyong; Yao, Liping

doi:10.1002/sca.20154

Cited by 11 publications

(11 citation statements)

References 26 publications

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“…Atomic force microscopy (AFM) is a powerful technique for structural characterization of biomolecules at single molecular level. Individual DNA molecule and protein has been characterized by AFM (Liu et al ., , , , ; Shi et al ., ). AFM can be further employed to study the interactions of the different drugs and DNA molecule.…”

Section: Introductionmentioning

confidence: 99%

Structural study on the interactions of oxaliplatin and linear DNA

Zou

Biao

et al. 2016

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Damage to cellular DNA is believed to determine the cytotoxicity of oxaliplatin. However, high resolution structures formed by oxaliplatin and different linear DNA remain unclear. This study characterized, the key structures of different linear DNA in the platination process by UV absorption spectra and atomic force microscopy (AFM). Bathochromic shift and hyperchromicity in UV spectra after addition of oxaliplatin revealed that it can disrupt base stacking of DNA in the platination process. AFM results of different linear DNA indicated that, the platination process can induce DNA change from an extended conformation to the network structure with many kinks and finally to the compact particles, or toroids with increasing the incubation time. All AFM results confirmed that, platination of different linear DNA by oxaliplatin is a time depended process. The present AFM results provide, structural evidence about the interactions between oxaliplatin and different linear DNA containing multiple targets. SCANNING 38:880-888, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Structural study on the interactions of oxaliplatin and linear DNA

Zou

Biao

et al. 2016

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“…Atomic force microscopy (AFM) is a very useful technique to study biomolecules at single molecular level. Structural features of single DNA molecule and protein have been investigated by AFM (Liu et al, 2005a(Liu et al, ,b, 2008(Liu et al, , 2009Shi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Study on the interactions between anti-cancer drug oxaliplatin and DNA by atomic force microscopy

Liu

et al. 2015

Micron

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“…Thus, structural studies of DNA molecules on HOPG are very helpful for understanding the experimental results on carbon nanotube and graphene. Atomic force microscopy (AFM) is a powerful tool for observation of DNA molecules and protein immobilized on a solid substrate since it has been invented (Rippe et al , '; Pope et al , '; Liu et al , , ). Recently, DNA structures formed on the alkylamines and a graphite modifier‐modified HOPG surface have been observed by AFM (Severin et al , ; Adamcik et al , , ; Klinov et al , ; Dubrovin et al , ).…”

Section: Introductionmentioning

confidence: 99%

Influence of Mg²⁺, Ni²⁺, and Cu²⁺ on DNA assembly on HOPG surfaces: atomic force microscopy study

Zhao

Liu

et al. 2011

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Adsorption of circular DNA onto bare highly oriented pyrolytic graphite (HOPG) surfaces by the addition of Mg²⁺, Ni²⁺, and Cu²⁺ has been investigated by atomic force microscopy (AFM). AFM results revealed that the topography and height of DNA on HOPG surface by the addition of different metal ions are quite different. After the addition of Mg²⁺ for incubation, DNA molecules tend to form many loops on HOPG surfaces, which are derived from the crossover of intramolecular and intermolecular chains. After the addition of Ni²⁺, DNA molecules can form network on HOPG surfaces, and the density of DNA network was significantly increased with increasing DNA concentration. Consequently, dense DNA network can be obtained by using relatively low concentration of DNA and Ni²⁺. As for the addition of Cu²⁺, angular DNA loops composed of flat chains were observed. The observed flat DNA chains with an average height of 0.52 nm can be ascribed to Cu²⁺ insert into the site between bases and phosphate group of DNA inducing denaturation of DNA molecules. This study is very helpful for understanding the interactions of metal ions and DNA molecules, and for constructing various DNA structures on the carbonaceous surfaces.

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Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy

Cited by 11 publications

References 26 publications

Structural study on the interactions of oxaliplatin and linear DNA

Structural study on the interactions of oxaliplatin and linear DNA

Study on the interactions between anti-cancer drug oxaliplatin and DNA by atomic force microscopy

Influence of Mg²⁺, Ni²⁺, and Cu²⁺ on DNA assembly on HOPG surfaces: atomic force microscopy study

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Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy

Cited by 11 publications

References 26 publications

Structural study on the interactions of oxaliplatin and linear DNA

Structural study on the interactions of oxaliplatin and linear DNA

Study on the interactions between anti-cancer drug oxaliplatin and DNA by atomic force microscopy

Influence of Mg2+, Ni2+, and Cu2+ on DNA assembly on HOPG surfaces: atomic force microscopy study

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Influence of Mg²⁺, Ni²⁺, and Cu²⁺ on DNA assembly on HOPG surfaces: atomic force microscopy study