RETRACTED: Comparison of Coconut Water, Propolis, HBSS, and Milk on PDL Cell Survival

Gopikrishna, Velayutham; Baweja, Parvinder Singh; Nagendrababu, Venkateshbabu; Thomas, Toby; Kandaswamy, Deivanayagam

doi:10.1016/j.joen.2008.01.018

Cited by 112 publications

(207 citation statements)

References 21 publications

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“…They attributed that increased cell damage to the cell lysis caused by the very low osmolarity of tap water [7]. Among the studied environments, tap water has shown to be the one with the least desirable results, though it protects the tooth from dehydration -for being a hypotonic medium -it causes rapid cellular death of the PDL, similarly to a dry storage [8].…”

Section: Tap Watermentioning

confidence: 99%

Current Developments in Transport Media for Avulsed Teeth: An Update

Roma

Hegde

2017

Asian J Pharm Clin Res

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Dental avulsion is one of the common types of trauma that results in the complete displacement of tooth from the alveolar socket. Although the ideal treatment would be the immediate reimplantation of the tooth at the site where the trauma took place, this may not be practically possible in every case. Hence, the avulsed tooth may have to be placed in an appropriate storage or transport medium until it is reimplanted. The biological properties of the storage medium have a significant impact on the success of reimplantation, as it must be capable of preserving the vitality, clonogenic, and mitogenic ability of the periodontal ligament cells for successful reimplantation.

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Section: Tap Watermentioning

confidence: 99%

Current Developments in Transport Media for Avulsed Teeth: An Update

Roma

Hegde

2017

Asian J Pharm Clin Res

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“…In this sense, various storage media have been investigated for their ability to maintain the viability of PDL cells for as long as possible. These storage media include milk, culture media, Viaspan, Hank's Balanced Salt Solution (HBSS), Gatorade®, propolis 8,9 , soy milk 10,11 , and coconut water (CW) [11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, some studies [11][12][13][14][15] have proposed CW as a promising storage medium for avulsed teeth. The natural, fresh and tender CW is a sterile solution, with physiological osmolarity used in the past as a blood plasma substitute 16 .…”

Section: Introductionmentioning

confidence: 99%

“…It can replace fluids, amino acids (lysine, cystine, phenylalanine, histidine, and tryptophan), sugars (fructose and glucose), and electrolytes (potassium, calcium, and magnesium) 12 . All these properties may explain its ability to preserve cell viability 12,13,17 . Despite the favorable characteristics of CW, studies involving this solution as a storage medium have shown contradictory results.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the favorable characteristics of CW, studies involving this solution as a storage medium have shown contradictory results. Some studies report that CW showed better performance than other storage media such as HBSS or milk in terms of maintaining PDL cell viability after avulsion [11][12][13]15,18 ; another study addressed low capability to preserve cells 14 . The pH adjustment of this solution seems to be an indispensable stage for its production at an industrial scale 9 .…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Soares

Rocha

Reis

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: Avaliar a eficácia de quatro tipos de substâncias usadas para ajuste do pH da água de coco (AC) sobre a viabilidade de fibroblastos humanos (HFF). Material e método: O pH da AC natural e industrializada foi ajustado para pH 7,0 utilizando: (1) Hidróxido de Sódio (NaOH), (2) bicarbonato de sódio (NaHCO3), (3) Trietanolamina (C6H15NO3), (4) 2-Amino-2-Methil-1-propanol (C4H11NO). Células HFF foram plaqueadas em 2×10 4 células/poço em placas de 96 poços e mantidas nas diferentes soluções de AC acima durante 2 h e 4 h. Controle positivo foi representado por HFF mantidas em DMEM e o controle negativo por água da torneira. A viabilidade celular foi avaliada pelo método de MTT Formazan. Os dados foram analisados por 3-way ANOVA seguido pelo teste de Tukey e Dunnett. Resultado: A viabilidade celular não é influenciada pelo período de avaliação, e as interações entre AC e período de avaliação, AC e método de ajuste de pH, método de ajuste de pH e período de avaliação (p>0,05). Conclusão: O produto utilizado para ajuste do pH não interfere na viabilidade de FH, embora, haja uma tendência de melhor desempenho em AC natural.Descritores: Avulsão dentária; água de coco; viabilidade celular. AbstractObjective: This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method: Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO 3 ), (3) Triethanolamine (C 6 H 15 NO 3 ), (4) 2-Amino-2-Methil-1-Propanol (C 4 H 11 NO). Fibroblasts were plated at 2×10 4 / well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey's and Dunnet's test. Result: There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion: The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

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