Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Abstract

ResumoObjetivo: Avaliar a eficácia de quatro tipos de substâncias usadas para ajuste do pH da água de coco (AC) sobre a viabilidade de fibroblastos humanos (HFF). Material e método: O pH da AC natural e industrializada foi ajustado para pH 7,0 utilizando: (1) Hidróxido de Sódio (NaOH), (2) bicarbonato de sódio (NaHCO3), (3) Trietanolamina (C6H15NO3), (4) 2-Amino-2-Methil-1-propanol (C4H11NO). Células HFF foram plaqueadas em 2×10 4 células/poço em placas de 96 poços e mantidas nas diferentes soluções de AC acima durante 2 h e 4 h. Controle positivo foi representado por HFF mantidas em DMEM e o controle negativo por água da torneira. A viabilidade celular foi avaliada pelo método de MTT Formazan. Os dados foram analisados por 3-way ANOVA seguido pelo teste de Tukey e Dunnett. Resultado: A viabilidade celular não é influenciada pelo período de avaliação, e as interações entre AC e período de avaliação, AC e método de ajuste de pH, método de ajuste de pH e período de avaliação (p>0,05). Conclusão: O produto utilizado para ajuste do pH não interfere na viabilidade de FH, embora, haja uma tendência de melhor desempenho em AC natural.Descritores: Avulsão dentária; água de coco; viabilidade celular. AbstractObjective: This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method: Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO 3 ), (3) Triethanolamine (C 6 H 15 NO 3 ), (4) 2-Amino-2-Methil-1-Propanol (C 4 H 11 NO). Fibroblasts were plated at 2×10 4 / well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey's and Dunnet's test. Result: There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion: The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

Replanted teeth stored in a newly developed powdered coconut water formula

“…The differences observed in histomorphometric analysis between G1 and G4 may be related to different pH control systems. Group G4, which used triethanolamine as a neutralizer agent, was included in the current research due to favourable results in previous studies . However, the use of this substance as a neutralizing agent is not feasible under home and school conditions.…”

“…The unfavourable performance of G3, similar to dry conditions (negative control), with higher values of external root resorption, is probably related to its acidic pH, which affects cell metabolism . Although the current literature shows conflicting results regarding the potential use of CW as a storage medium, recent studies confirm the importance of adjusting the pH of the CW, which has proven to be an essential step for its use as a storage medium for avulsed teeth.…”

“…Group G4, which used triethanolamine as a neutralizer agent, was included in the current research due to favourable results in previous studies. [9][10][11][12] However, the use of this substance as a neutralizing agent is not feasible under home and school conditions. Additionally, G3 had rapid deterioration even in the long shelf life preparation, rendering its use as a tooth preservation medium unfeasible.…”

“…4 However, CW possesses an acidic pH that compromises cell metabolism, 3,8 and thus, it is important to use some substance capable of maintaining a near-neutral pH for the longest duration possible. 7,9 Considering this requirement, studies have been performed using sodium hydrogen carbonate (NaHCO 3 ) 7 and triethanolamine [9][10][11][12] to neutralize the pH of CW, allowing its use as a storage medium for avulsed teeth. However, it is not possible to adjust the pH of CW to 7.0 in domestic conditions.…”