RETRACTED ARTICLE: miR-484 suppresses proliferation and epithelial–mesenchymal transition by targeting ZEB1 and SMAD2 in cervical cancer cells

Hu, Yang; Xie, Hong; Liu, Yankun; Liu, Weiying; Liu, Min; Tang, Hua

doi:10.1186/s12935-017-0407-9

Cited by 62 publications

(54 citation statements)

References 43 publications

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“…Recent studies showed that miR-484 play important roles in tumor progression. For example, Hu et al showed that miR-484 suppressed proliferation and epithelial-mesenchymal transition by targeting ZEB1 and SMAD2 in cervical cancer cells [25]. While, Li et al showed that miR-484 promoted non-small-cell lung cancer progression through inhibiting Apaf-1 associated with the suppression of apoptosis [26].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer

Xie

Wang

et al. 2018

Cell Cycle

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The incidence and mortality rate of colorectal cancer (CRC) have been significantly increasing. However, mechanisms involved in CRC progression are still unclear. LncRNA ZFAS1 has been verified as oncogenic molecular in a series of tumors, including CRC. However, the underlying mechanism of ZFAS1 in CRC carcinogenesis remains unclear. In the present study, our data showed that ZFAS1 expression was significantly upregulated in CRC tissues and cell lines. Correlation analysis showed that high ZFAS1 expression was significantly associated with Helicobacter pylori infection, lymph nodes metastasis, advanced TNM stage and poor overall survival of CRC patients. Loss-of-function experiments revealed that ZFAS1 inhibition could markedly suppress CRC cells proliferation and invasion both in vitro and in vivo. Bioinformatics analysis and luciferase reporter assay revealed that ZFAS1 directly interacted with miR-484. Rescue experiments showed that miR-484 inhibitor reversed the tumor suppressing roles of ZFAS1 knockdown on CRC cells. Therefore, our study suggested that ZFAS1 could act as an oncogene in CRC tumorigenesis, and discovered the functional regulatory pathway of ZFAS1 sponging miR-484.

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer

Xie

Wang

et al. 2018

Cell Cycle

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“…been illustrated in multiple cancers including cervical cancer. 6,7 LncRNAs are generally defined as RNA transcripts with more than 200 nucleotides in length and no proteincoding potential. Increasing evidence has indicated that lncRNAs are implicated in a variety of pathophysiological processes, such as gene expression, cell proliferation, apoptosis, and tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

LncRNA GAS5 suppresses the tumorigenesis of cervical cancer by downregulating miR-196a and miR-205

Yang

et al. 2017

Tumour Biol.

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Growth arrest special 5 (GAS5) is a long non-coding RNA reported to function as an inhibitor in various tumors including cervical cancer. However, the molecular mechanism of GAS5 involved in cervical cancer progression remains far from being elucidated. The expression of GAS5, forkhead box protein O1 and phosphatase and tensin homolog was examined by quantitative reverse transcription polymerase chain reaction qRT-PCR. cell growth, invasion, and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, transwell invasion assay, and flow cytometry analysis, respectively. The interaction between GAS5 and miR-196a or miR-205 was confirmed by luciferase reporter assay, RNA immunoprecipitation assay, and qRT-PCR. Xenograft tumor experiments were performed to validate the biological role of GAS5 and its molecular mechanism in cervical cancer in vivo. GAS5 expression was decreased in cervical cancer tissues and cells. GAS5 overexpression suppressed cervical cancer cell proliferation, invasion, and apoptosis. GAS5 was able to directly bind to miR-196a and miR-205 to downregulate their expression. Moreover, GAS5 induced forkhead box protein O1 and phosphatase and tensin homolog expression by repressing miR-196a and miR-205, respectively. Exogenous expression of GAS5 hindered tumor growth in vivo by downregulating miR-196a and miR-205. Upregulation of GAS5 suppressed cell proliferation, invasion, and apoptosis of cervical cancer cells by downregulating miR-196a and miR-205, contributing to our understanding the pathogenesis of cervical cancer and development of long non-coding RNA–mediated clinical therapy against this disease.

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“…The abnormal expression of circulating miRNA has also been widely reported in patients with NSCLC (6,26). Increasing evidence has indicate the important role of miR-484 in various types of tumor (18,27,28). For instance, increased miR-484 levels have been revealed to promote glioma progression by regulating extracellular signal-regulated kinase 1/2 signaling (27).…”

Section: Discussionmentioning

confidence: 99%

“…In metastatic renal cell carcinoma, miR-484 has been reported to be increased and associated with tumor progression (28). However, in cervical cancer, miR-484 serves as a tumor suppressor by suppressing zinc finger E-box-binding homeobox 1 and SMAD2 (18). Circulating miRNA exhibits a good stability in serum, which is associated with the occurrence and development of tumors (29).…”

Section: Discussionmentioning

confidence: 99%

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High serum miR‑484 expression is associated with the diagnosis and prognosis of patients with non‑small cell lung cancer

2019

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The aim of the current study was to assess the expression and clinical significance of serum microRNA (miR)-484 in patients with non-small cell lung cancer (NSCLC). Reverse transcription-quantitative polymerase chain reaction was performed to determine the expression of miR-484 in the serum of patients with NSCLC and NSCLC cell lines. Cell counting kit-8, flow cytometry, cell migration and cell invasion assays were performed to assess the role of miR-484 in the malignant changes associated with NSCLC cells. Furthermore, to assess the diagnostic value of miR-484, receiver operating curve (ROC) analysis was performed and the clinical relevance of serum miR-484 expression in patients with NSCLC was determined. A Kaplan-Meier analysis with the log-rank test was performed to assess the overall survival rate patients. To the best of our knowledge, the current study demonstrates for the first time that serum miR-484 was increased in patients with NSCLC compared with healthy controls. Additionally, serum miR-484 was revealed to be positively associated with histological grade, lymph node metastasis, distant metastasis and clinical stage. Patients with NSCLC and high serum miR-484 levels demonstrated significantly poorer overall survival rates compared with those exhibiting lower serum miR-484 expressions. ROC analysis revealed that serum miR-484 could screen patients with NSCLC patients from healthy controls with a high sensitivity and specificity. In vitro analysis also demonstrated that miR-484 was significantly upregulated in NSCLC cell lines, including 95D and H358 cells. Furthermore, the suppression of miR-484 decreased cell proliferation, cell migration and invasion. In summary, the results of the present study demonstrated that increased serum miR-484 expression is associated with the diagnosis and prognosis of patients with NSCLC. High serum miR-484 expression is associated with the diagnosis and prognosis of patients with non-small cell lung cancer

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RETRACTED ARTICLE: miR-484 suppresses proliferation and epithelial–mesenchymal transition by targeting ZEB1 and SMAD2 in cervical cancer cells

Cited by 62 publications

References 43 publications

Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer

Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer

LncRNA GAS5 suppresses the tumorigenesis of cervical cancer by downregulating miR-196a and miR-205

High serum miR‑484 expression is associated with the diagnosis and prognosis of patients with non‑small cell lung cancer

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