Long non-coding RNA ZFAS1 sponges miR-484 to promote cell proliferation and invasion in colorectal cancer

Abstract

The incidence and mortality rate of colorectal cancer (CRC) have been significantly increasing. However, mechanisms involved in CRC progression are still unclear. LncRNA ZFAS1 has been verified as oncogenic molecular in a series of tumors, including CRC. However, the underlying mechanism of ZFAS1 in CRC carcinogenesis remains unclear. In the present study, our data showed that ZFAS1 expression was significantly upregulated in CRC tissues and cell lines. Correlation analysis showed that high ZFAS1 expression was significantly associated with Helicobacter pylori infection, lymph nodes metastasis, advanced TNM stage and poor overall survival of CRC patients. Loss-of-function experiments revealed that ZFAS1 inhibition could markedly suppress CRC cells proliferation and invasion both in vitro and in vivo. Bioinformatics analysis and luciferase reporter assay revealed that ZFAS1 directly interacted with miR-484. Rescue experiments showed that miR-484 inhibitor reversed the tumor suppressing roles of ZFAS1 knockdown on CRC cells. Therefore, our study suggested that ZFAS1 could act as an oncogene in CRC tumorigenesis, and discovered the functional regulatory pathway of ZFAS1 sponging miR-484.

ZFAS1 knockdown inhibits viability and enhances cisplatin cytotoxicity by up‐regulating miR‐432‐5p in glioma cells

“…For instance, ZFAS1 interacted with miR‐150‐5p to regulate the expression of transcription factor Sp1, thereby regulating proliferation rate, migration activity and development of chemoresistance in epithelial ovarian cancer . ZFAS1 sponged miR‐484 to regulate cell proliferation and invasion in colorectal cancer cells and modulate tumour growth in nude mice . In addition, ZFAS1 served as an oncogene in bladder cancer tumorigenesis by sponging miR‐329 …”

“…26 ZFAS1 sponged miR-484 to regulate cell proliferation and invasion in colorectal cancer cells and modulate tumour growth in nude mice. 27 In addition, ZFAS1 served as an oncogene in bladder cancer tumorigenesis by sponging miR-329. 28 In the current study, the miR-432-5p was predicted as a target of ZFAS1.…”

Long noncoding RNA ZFAS1 enhances adriamycin resistance in pediatric acute myeloid leukemia through the miR-195/Myb axis

“…[9][10][11] Zinc nger antisense 1 (ZFAS1) is a novel tumor-related lncRNA that has been identied as an oncogene in the development of multiple human cancers. [12][13][14] Previous studies had demonstrated that ZFAS1 was abnormally expressed in AML, and ZFAS1 overexpression promoted AML progression through acting as a sponge for miR-150. 15,16 A recent document manifested that ZFAS1 promoted cervical cancer cell chemoresistance to cisplatin.…”

Long non‑coding RNA LINC01503 promotes colorectal cancer cell proliferation and invasion by regulating miR‑4492/FOXK1 signaling

“…Fu et al (9) indicated that long intergenic non-protein coding RNA (LINC)00210 drives Wnt/β-catenin signaling activation and liver tumor progression in a catenin β interacting protein 1-dependent manner. In addition, Xie et al (10) indicated that lncRNA zinc finger NFX1-type containing 1 antisense RNA 1 sponges miR-484 to promote cell proliferation and invasion in CRC. Therefore, it has been widely acknowledged that lncRNAs have essential roles in human cancer, including CRC.…”