RETRACTED ARTICLE: miR-381 modulates human bone mesenchymal stromal cells (BMSCs) osteogenesis via suppressing Wnt signaling pathway during atrophic nonunion development

Long, Haitao; Zhu, Yong; Lin, Zhenghua; Wan, Jun; Cheng, Liang; Zeng, Min; Tang, Yifu; Zhao, Ruibo

doi:10.1038/s41419-019-1693-z

Cited by 47 publications

(44 citation statements)

References 44 publications

(46 reference statements)

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“…In this context, miR-9-5p also promotes the occurrence and progression of osteoporosis through inhibiting osteogenesis and promoting adipogenesis via targeting Wnt3a in MSCs [ 131 ]. Wnt5a and FZD3 expression has also recently been found to be inhibited by miR-38, and binding to the mRNA 3′UTR hampered osteogenic differentiation of BM-MSCs [ 132 ]. The same authors also reported the involvement of miR-139-5p in hBM-MSC osteogenesis by directly targeting the β-catenin gene and frizzled 4 (FZD4) [ 133 ].…”

Section: Osteogenic Differentiation By Mirna Regulationmentioning

confidence: 99%

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

Mazziotta

Lanzillotti

Iaquinta

et al. 2021

IJMS

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Mesenchymal stem cells (MSCs) have been identified in many adult tissues and they have been closely studied in recent years, especially in view of their potential use for treating diseases and damaged tissues and organs. MSCs are capable of self-replication and differentiation into osteoblasts and are considered an important source of cells in tissue engineering for bone regeneration. Several epigenetic factors are believed to play a role in the osteogenic differentiation of MSCs, including microRNAs (miRNAs). MiRNAs are small, single-stranded, non-coding RNAs of approximately 22 nucleotides that are able to regulate cell proliferation, differentiation and apoptosis by binding the 3′ untranslated region (3′-UTR) of target mRNAs, which can be subsequently degraded or translationally silenced. MiRNAs control gene expression in osteogenic differentiation by regulating two crucial signaling cascades in osteogenesis: the transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) and the Wingless/Int-1(Wnt)/β-catenin signaling pathways. This review provides an overview of the miRNAs involved in osteogenic differentiation and how these miRNAs could regulate the expression of target genes.

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Section: Osteogenic Differentiation By Mirna Regulationmentioning

confidence: 99%

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

Mazziotta

Lanzillotti

Iaquinta

et al. 2021

IJMS

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“…Although there are currently no clear results showing that miR-381 regulates preadipocyte differentiation, many studies suggest its involvement in regulating lipid metabolism. Long et al [46] studied the effect of miR-381 on osteogenic differentiation and found that miR-381 overexpression increases PPARγ levels while inhibiting the Wnt signaling pathway. Other studies report that miR-381 inhibits the Wnt/β-catenin pathway by targeting cullin 4B [47].…”

Section: Discussionmentioning

confidence: 99%

miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

Shao

Fang

et al. 2020

Horm Metab Res

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MicroRNAs (miRNAs) are small, single-stranded, noncoding RNAs ~21 to ~23 nucleotides in length and have become a popular research topic in recent years due to their regulation of gene expression and many physiological processes, including fat metabolism; however, the precise functional mechanisms underlying their regulation of fat metabolism are not fully understood. Here, we identified miR-381, which specifically targets the 3′ untranslated region (3′ UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. The results showed that miR-381 directly targets the 3′ UTR of KCTD15 and downregulates its expression. Additionally, miR-381 overexpression using an miRNA mimic promoted triglyceride accumulation and upregulated adipogenic peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) at both the protein and mRNA levels, whereas miR-381 inhibition produced the opposite effect. These results indicated that miR-381 regulates the differentiation of Yanbian yellow cattle preadipocytes by inhibiting KCTD15 expression, thereby highlighting the importance of miRNA-mediated regulation of adipogenesis. Furthermore, our findings suggested that miR-381 and its target gene(s) might represent new targets for investigating intramuscular fat deposits in cattle and treating human obesity.

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“…Dozens of miRNAs have potential to regulating Wnt β-catenin signaling pathway. Long et al [20] revealed that miR-381 modulates hBMMSC osteogenesis via suppressing Wnt signaling pathway during atrophic nonunion development. Li et al [21] found that miR-23a inhibits osteogenic differentiation of hBMMSCs by targeting LRP5 (Wnt receptor).…”

Section: Discussionmentioning

confidence: 99%

The mechanism of miR-889 regulates osteogenesis in human bone marrow mesenchymal stem cells

Ding

Shi

2019

J Orthop Surg Res

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Background: Bone marrow mesenchymal stem cells (BMMSCs) can be used for bone regeneration in the specified condition. Osteogenic differentiation of BMMSCs is controlled by microRNAs (miRNAs) and other factors. This study was aimed to identify the role and mechanism of miR-889 in regulating the osteogenic differentiation of BMMSCs. Methods: Osteoporosis patients and normal control bone tissues were collected and used PCR techniques to identify the change of miR-889 and WNT7A. Moreover, the dynamic change of miR-889 and WNT7A during osteogenic differentiation of BMMSCs was also measured. Bioinformatic analysis was performed to identify the target genes and potential pathways of miR-889. Then, we constructed miR-889 mimic and inhibitor, ALP staining, ARS, osteoblastic-related protein, and Wnt β-catenin signaling pathway-related protein were also measured. WNT7A siRNA was also used to verify the function of miR-889. Results: In the present study, we showed that miR-889 expression was upregulated in osteoporosis patients than healthy control. However, the miR-889 expression was downregulated during osteogenic differentiation. Bioinformatics analysis found that miR-889 targets 666 genes and mainly through Wnt β-catenin signaling pathway. Administrated miR-889 mimic, the ALP activity, and calcium deposition were decreased than the control group, while miR-889 inhibitor shown the opposite trend. And miR-889 could bind the 3′UTR of WNT7A. We further used WNT7A siRNA to explore the function of miR-889, and the results revealed that co-cultured with miR-889 inhibitor and WNT7A siRNA was associated with a reduction of ALP activity and calcium deposition and osteoblastic-related proteins than miR-889 inhibitor alone. Conclusion: Our results revealed that miR-889 plays a negative role in inducing osteogenic differentiation of BMSCs through Wnt β-catenin signaling pathway.

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RETRACTED ARTICLE: miR-381 modulates human bone mesenchymal stromal cells (BMSCs) osteogenesis via suppressing Wnt signaling pathway during atrophic nonunion development

Cited by 47 publications

References 44 publications

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

The mechanism of miR-889 regulates osteogenesis in human bone marrow mesenchymal stem cells

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