miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

Xu, Hongyan; Shao, Jing; Fang, Jiachen; Yin, Baozhen; Zhang, Luomeng; Zhang, Jia-su; Xia, Guangjun

doi:10.1055/a-1276-1602

Cited by 6 publications

(7 citation statements)

References 44 publications

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“…MiR-25 repressed the expression of PGC-1beta to modulate triacylglycerol and lipid accumulation in goat mammary epithelial cells [ 30 ]. MiR-381 [ 31 ] targeted KCTD15 to promoted triglyceride accumulation through in vitro culture bovine preadipocyte. Meanwhile, miR-432 [ 32 ] was highly expressed in back fat of cattle, which targeted PRKAA1/2 , PPARA and PPARG to modulate lipid and fatty acid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome reveals key microRNAs involved in fat deposition between different tail sheep breeds

et al. 2022

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MicroRNA (miRNA) is a kind of noncoding RNA whose function involved in various biological processes in neuronal maturation and adipocyte cells, such as differentiation, proliferation, development, apoptosis, and metabolism. Herein, miRNA-Seq was used to identify miRNAs in the tail fat tissue of Hu sheep (short-fat-tailed) and Tibetan sheep (short-thin-tailed). In this study, 155 differentially expression miRNAs (DE miRNAs) were identified, including 78 up-regulated and 77 down-regulated. Among these DE miRNAs, 17 miRNAs were reported and related with lipid metabolism. MiRanda and RNAhybrid software were used to predict the target genes of DE miRNAs, obtaining the number of targeting relationships is 38553. Target genes were enriched by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 742 terms and 302 single pathways are enriched, including lipid metabolic process, response to lipid, cellular lipid catabolic process, lipid catabolic process, cellular lipid metabolic process, inositol lipid-mediated signaling, calcium channel activity, PI3K-Akt signaling pathway, MAPK signaling pathway, ECM-receptor interaction, AMPK signaling pathway, Wnt signaling pathway and TGF-beta signaling pathway. Notably, miR-379-5p was associated with tail fat deposition of sheep. Dual-Luciferase reporter assays showed miR-379-5p and HOXC9 had targeted relationship. The result of RT-qPCR showed that the expression trend of miR-379-5p and HOXC9 was opposite. miR-379-5p was down-regulated and highly expressed in tail adipose tissue of Tibetan sheep. HOXC9 was highly expressed in adipose tissue of Hu sheep. These results could provide a meaningful theoretical basis for studying the molecular mechanisms of sheep tail adipogenesis.

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Section: Discussionmentioning

confidence: 99%

Transcriptome reveals key microRNAs involved in fat deposition between different tail sheep breeds

et al. 2022

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“…At present, to the best of our knowledge, there have been no reports about the role of miR-381 in myogenic differentiation in other animals. However, miR-381 has been reported to promote the differentiation of other types of cells, including cattle preadipocytes [ 19 ], mouse embryonic palatal mesenchymal cells [ 20 ] and human retinal progenitor cells [ 21 ]. These studies further support our findings.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2

Wang

Zhen

Liu

et al. 2022

IJMS

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In our previous study, microRNA (miR)-381 was found to be the most down-regulated miRNA in skeletal muscle of Liaoning cashmere goats with higher skeletal muscle mass, but the molecular mechanism involved remains unclear. In this study, primary caprine skeletal muscle satellite cells (SMSCs) were isolated and identified. We investigated the effect of miR-381 on the viability, proliferation and differentiation of caprine SMSCs, and the target relationships of miR-381 with jagged canonical Notch ligand 2 (JAG2) and phosphatase and tensin homolog (PTEN). Cells isolated were positive for SMSC-specific marker protein Pax7. This suggests that purified SMSCs were obtained. The expression level of miR-381 achieved a peak value on day 4 after SMSC differentiation, and miR-381 also significantly increased the expression levels of myogenic differentiation marker genes: myosin heavy chain (MyHC), myogenin (MyoG) and myocyte enhancer factor 2C (MEF2C) in differentiated SMSCs, the area of MyHC-positive myotubes and the myogenic index. These findings suggest that miR-381 promoted myogenic differentiation of caprine SMSCs. The CCK8 assay and EDU staining analysis showed that miR-381 mimic both inhibited the viability of SMSCs and decreased the percentage of EDU-labeled positive SMSCs. In contrast, miR-381 inhibitor had the opposite effect with miR-381 mimic. A dual luciferase reporter assay verified that miR-381 can target JAG2 and PTEN by binding to the 3′-untranslated regions (3′-UTR) of the genes. The transfection of miR-381 mimic into caprine SMSCs resulted in decreases in expression levels of JAG2 and PTEN, while miR-381 inhibitor increased the two target genes in expression. This is the first study to reveal the biological mechanisms by which miR-381 regulates caprine SMSC activities.

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“…Xu et al [19] cultured Yanbian yellow cattle precursor adipocytes in vitro and transfected miRNA and found that miR-1271 could inhibit the expression of the ATF3 gene and promote the adipogenic differentiation of precursor adipocytes. In addition, miR-381 can also inhibit the expression of the KCTD15 gene [20]. Wang et al [21] found that the overexpression of miR-223 in chicken liver cells LMH reduced the mRNA and protein expression of DAGLA.…”

Section: Discussionmentioning

confidence: 99%

miR-15a Targets ABAT Gene to Regulate the Differentiation of Preadipocytes of Yanbian Yellow Cattle

Bai

Fang

et al. 2021

Preprint

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BackgroundMicroRNAs (miRNAs) are small RNAs that cannot be translated into proteins. The length of a mature miRNA is approximately 20-25 nt. Neural regulation, inflammatory response, cell proliferation and apoptosis, fat metabolism, and cell differentiation, among others, have become research hotspots in recent years. In this experiment, dual-luciferase reporter gene detection, qRT-PCR, oil red O staining, western blotting, and other methods were used to select the differentially expressed miR-15a and its target gene, the 4-aminobutyrate aminotransferase (ABAT) gene, in the longissimus muscle tissues of cattle bulls and steers. By transfecting Yanbian yellow cattle precursor adipocytes with equal amounts of artificially synthesised miR-15a mimics, mimics NC, miR-15a inhibitor, and inhibitor NC and inducing differentiation, the total RNA and total protein of cells during the induced differentiation process were extracted.The effect of miR-15a on the mRNA and protein expression of adipogenesis-related genes was analysed. ResultsThe results showed that the lipid droplet content and lipid droplet size of the miR-15a mimic group were significantly lower than those of the mimics NC group, and the mRNA levels of the peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) genes in the miR-15a mimics group were significantly lower than those in the mimics NC group. At the same time, the protein expression of miR-15a mimic group cells was also significantly lower than that of the NC group cells. Meanwhile, the mRNA levels of the PPARγ and C/EBPα genes of miR-15a inhibitor group cells were significantly higher than those of the NC group cells, and the protein expression levels were also significantly higher than those of the NC group cells. The luciferase reporter results showed that miR-15a mimics significantly inhibited the activity of the ABAT-3'UTR dual luciferase reporter gene vector, indicating that the miR-15a and ABAT genes have a targeting effect, and that overexpressed miR-15a significantly reduced the expression of the ABAT gene in the cell, inhibited the expression of miR-15a, and significantly increased the expression of the ABAT.ConclusionsIn summary, miR-15a may target the ABAT gene to regulate the differentiation of Yanbian yellow cattle precursor adipocytes.

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miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

Cited by 6 publications

References 44 publications

Transcriptome reveals key microRNAs involved in fat deposition between different tail sheep breeds

Transcriptome reveals key microRNAs involved in fat deposition between different tail sheep breeds

MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2

miR-15a Targets ABAT Gene to Regulate the Differentiation of Preadipocytes of Yanbian Yellow Cattle

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