RETRACTED ARTICLE: MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway

Wei, Chunhua; Wu, Gang; Cai, Qian; Gao, Xi; Tong, Fan; Zhou, Rui; Zhang, Ruiguang; Dong, Jing; Hu, Yu; Dong, Xiaorong

doi:10.1186/s13045-017-0493-0

Cited by 77 publications

(55 citation statements)

References 51 publications

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“…Pathway analysis of the total of 339 genes showed that altered genes are largely involved in cell division and cell cycle regulation (Table 1). For two hypermethylated genes in our analysis, ESX1, a homeodomain protein, was reported to regulate cell cycle progression and transcription [24-26], and GRIA3 has been shown to promote proliferation in nonneuronal cells [27, 28]. As other glutamate receptors, such as NMDA receptor, have an important function in podocyte biology and glomerular diseases, we selected GRIA3 for further analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Kidney sections and isolated glomeruli were obtained from control (control-eNOS –/– ) and diabetic (STZ-eNOS –/– ) mice as described previously [27]. a Real-time PCR of Gria3 in isolated glomeruli is shown as a relative fold change to control mice.…”

Section: Resultsmentioning

confidence: 99%

“…We now show that GRIA3 is epigenetically regulated in podocytes under HG conditions, and that GluR3, encoded by GRIA3 , confers protection against podocyte apoptosis in HG conditions. Recent reports indicate that GluR3 promotes cell growth through activation of MAPK pathway [27]. Therefore, GluR3 may be a survival factor for podocytes through activation of the MAPK pathway [34, 35].…”

Section: Discussionmentioning

confidence: 99%

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Expression of Glutamate Receptor Subtype 3 Is Epigenetically Regulated in Podocytes under Diabetic Conditions

Li¹,

Chen²,

Zhong³

et al. 2018

Kidney Dis

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Background: Recent studies suggest a role of epigenetics in the pathogenesis of diabetic kidney disease. However, epigenetic changes occurring specifically in kidney cells is poorly understood. Methods: To examine the epigenetic regulation of genes in podocytes under diabetic conditions, we performed DNA methylation and transcriptomic profiling in podocytes exposed to high glucose conditions. Results: Comparative analysis of genes with DNA methylation changes and correspondingly altered mRNA expression identified 337 hypomethylated genes with increased mRNA expression and only 2 hypermethyated genes (ESX1 and GRIA3) with decreased mRNA expression. Glutamate ionotropic receptor AMPA type subunit 3 (GRIA3) belongs to the ionotropic class of glutamate receptors that mediate fast excitatory synaptic transmission in the central nervous system. As podocytes have glutamate-containing vesicles and various glutamate receptors mediate important biological effects in podocytes, we further examined GRIA3 expression and its function in podocytes. Real-time PCR and western blots confirmed the suppression of GRIA3 expression in podocytes under high glucose conditions, which were abolished in the presence of a DNA methyltransferase inhibitor. Sites of DNA hypermethylation were also confirmed by bisulfite sequencing of the GRIA3 promoter region. GRIA3 mRNA and protein expression was suppressed in diabetic kidneys of human and mouse models, and knockdown of GRIA3 exacerbated high glucose-induced apoptosis in cultured podocytes. Conclusion: These results indicate that decreased GRIA3 expression in podocytes in diabetic condition heightens podocyte apoptosis and loss.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of Glutamate Receptor Subtype 3 Is Epigenetically Regulated in Podocytes under Diabetic Conditions

Li¹,

Chen²,

Zhong³

et al. 2018

Kidney Dis

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“…It was reported that miR-330-3p can target the gene of program cell death 4 (PDCD4) to promote tumor [17]. MiR-330-3p is also related with metastasis and invasion of lung cancer, and glutamate receptor 3 (GRIA3) [18] and human manganese superoxide dismutase 2 (hSOD2) [19] are its two target genes. Researches found miR-330-3p is correlated with bad prognosis in breast cancer as well [20], in which miR-330-3p can target Collagen and Calcium Binding EGF Domains 1 (CCBE1) to promote cancer metastasis [21].…”

Section: Discussionmentioning

confidence: 99%

Expression profile of SYNE3 and bioinformatic analysis of its prognostic value and functions in tumors

Liao

Zhang

Yang

et al. 2020

Preprint

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Background: Spectrin repeat containing nuclear envelope family member 3 (SYNE3) encodes an important component of linker of cytoskeleton and nucleoskeleton (LINC) complex, namely nesprin-3. In tumor, invasiveness and metastasis rely on the integrity of LINC complex, while the role of SYNE3/nesprin-3 in cancer is rarely studied. Methods: Here, we explored the expression pattern, prognostic value and related mechanisms of SYNE3 through both experimental and bioinformatic methods. We first detected SYNE3 in BALB/c mice, normal human tissues and the paired tumor tissues, then used bioinformatic databases to verify our results. We further analyzed the prognostic value of SYNE3. Next, we predicted miRNA targeting SYNE3 and built a competing endogenous RNA (ceRNA) network and a transcriptional network by analyzing data from the cancer genome atlas (TCGA) database. Interacting genes of SYNE3 were predicted, and we further performed GO and KEGG enrichment analysis on these genes. Besides, the relationship of SYNE3 and immune infiltration was also investigated. Results: SYNE3 exhibited various expressions in different tissues, mainly located on nuclear and in cytoplasm sometimes. SYNE3 expression level had prognostic value in tumors, possibly by stablizing nucleus, promoting tumor cells apoptosis and altering tumor microenvironment. Additionally, we constructed a RP11-2B6.2-miR-149-5p-/LINC01094-miR-330-3p-SYNE3 ceRNA network and a SATB1-miR-149-5p-SYNE3 transcriptional network in lung squamous cell carcinoma to support the tumor-suppressing role of SYNE3. Conclusions: Our study explored novel anti-tumor functions and mechanisms of SYNE3, which might be useful for future cancer therapy.

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“…In addition, miR‐330‐3p is increased in non–small‐cell lung cancer (NSCLC) patient tissues (Arora et al, ). MiR‐330‐3p promotes NSCLC cell invasion and metastasis by activating MAPK/ERK (Wei et al, ). MiR‐330‐3p is elevated in plasma samples from patients with gestational diabetes mellitus (Sebastiani et al, ).…”

Section: Discussionmentioning

confidence: 99%

DGCR5 attenuates neuropathic pain through sponging miR‐330‐3p and regulating PDCD4 in CCI rat models

Peng

Zhang

et al. 2018

Journal Cellular Physiology

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Neuropathic pain caused by somatosensory nervous system dysfunction is a serious public health problem. Some long noncoding RNAs (lncRNAs) can participate in physiological processes involved in neuropathic pain. However, the effects of lncRNA DGCR5 in neuropathic pain have not been explored. Therefore, in our current study, we concentrated on the biological roles of DGCR5 in neuropathic pain. Here, it was observed that DGCR5 was significantly decreased in chronic sciatic nerve injury (CCI) rat models. DGCR5 overexpression was able to alleviate neuropathic pain development including mechanical and thermal hyperalgesia. In addition, the current understanding of miR-330-3p function in neuropathic pain remains largely incomplete. Here, we found that miR-330-3p was greatly increased in CCI rats and DGCR5 can modulate miR-330-3p expression negatively. Upregulation of DGCR5 repressed inflammation-correlated biomarkers including interleukin 6 (IL-6), tumor necrosis factor α, and IL-1β in CCI rats by sponging miR-330-3p. The negative correlation between DGCR5 and miR-330-3pwas confirmed in our current study. Inhibition of miR-330-3p suppressed neuropathic pain progression by restraining neuroinflammation in vivo. In addition, PDCD4 was predicted as a downstream target of miR-330-3p. Furthermore, PDCD4 was significantly increased in CCI rats and DGCR5 regulated PDCD4 expression through sponging miR-330-3p in CCI rat models. Taken these together, it was implied that DGCR5/miR-330-3p/PDCD4 axis participated in neuropathic pain treatment. K E Y W O R D SDGCR5, miR-330-3p, neuropathic pain, PDCD4

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RETRACTED ARTICLE: MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway

Cited by 77 publications

References 51 publications

Expression of Glutamate Receptor Subtype 3 Is Epigenetically Regulated in Podocytes under Diabetic Conditions

Expression of Glutamate Receptor Subtype 3 Is Epigenetically Regulated in Podocytes under Diabetic Conditions

Expression profile of SYNE3 and bioinformatic analysis of its prognostic value and functions in tumors

DGCR5 attenuates neuropathic pain through sponging miR‐330‐3p and regulating PDCD4 in CCI rat models

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