Expression profile of SYNE3 and bioinformatic analysis of its prognostic value and functions in tumors

Abstract

Background: Spectrin repeat containing nuclear envelope family member 3 (SYNE3) encodes an important component of linker of cytoskeleton and nucleoskeleton (LINC) complex, namely nesprin-3. In tumor, invasiveness and metastasis rely on the integrity of LINC complex, while the role of SYNE3/nesprin-3 in cancer is rarely studied. Methods: Here, we explored the expression pattern, prognostic value and related mechanisms of SYNE3 through both experimental and bioinformatic methods. We first detected SYNE3 in BALB/c mice, normal human tissues and the paired tumor tissues, then used bioinformatic databases to verify our results. We further analyzed the prognostic value of SYNE3. Next, we predicted miRNA targeting SYNE3 and built a competing endogenous RNA (ceRNA) network and a transcriptional network by analyzing data from the cancer genome atlas (TCGA) database. Interacting genes of SYNE3 were predicted, and we further performed GO and KEGG enrichment analysis on these genes. Besides, the relationship of SYNE3 and immune infiltration was also investigated. Results: SYNE3 exhibited various expressions in different tissues, mainly located on nuclear and in cytoplasm sometimes. SYNE3 expression level had prognostic value in tumors, possibly by stablizing nucleus, promoting tumor cells apoptosis and altering tumor microenvironment. Additionally, we constructed a RP11-2B6.2-miR-149-5p-/LINC01094-miR-330-3p-SYNE3 ceRNA network and a SATB1-miR-149-5p-SYNE3 transcriptional network in lung squamous cell carcinoma to support the tumor-suppressing role of SYNE3. Conclusions: Our study explored novel anti-tumor functions and mechanisms of SYNE3, which might be useful for future cancer therapy.

Parthenolide inhibits the tumor characteristics of renal cell carcinoma

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High SPATA18 Expression and its Diagnostic and Prognostic Value in Clear Cell Renal Cell Carcinoma

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WT1 Inhibits Human Renal Carcinoma Cell Proliferation and Induces G2/M Arrest by Upregulating IL-24 Expression

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