Resveratrol prevents TNF-α-induced VCAM-1 and ICAM-1 upregulation in endothelial progenitor cells via reduction of NF-κB activation

Abstract

Objective To assess the effects of resveratrol (RSV) on expression of adhesion molecules in endothelial progenitor cells (EPCs) following tumor necrosis factor-α (TNF-α) stimulation. Methods EPCs were treated with RSV and stimulated with TNF-α. A mononuclear cell (MNC) adhesion assay was used to assess the effects of RSV on TNF-α-induced MNC adhesion. Vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and E-selectin expression levels and nuclear factor κB (NF-κB) activation were assessed by immunoblotting. Results MNC adhesion to TNF-α-treated EPCs and VCAM-1/ICAM-1/E-selectin levels in EPCs were increased following TNF-α stimulation and decreased following RSV treatment. TNF-α enhanced NF-κB inhibitor α (IκB-α) phosphorylation in the cytosol as well as nuclear NF-κB p65 levels, both of which were decreased by RSV. Conclusions These findings provide new insights into RSV’s anti-inflammatory and anti-atherosclerotic effects. RSV’s mechanism of action might involve downregulation of VCAM-1, ICAM-1 and E-selectin by partial blockade of TNF-α-induced NF-κB activation and IκB-α phosphorylation in EPCs.

The Effect of Dietary Polyphenols on Vascular Health and Hypertension: Current Evidence and Mechanisms of Action

“…Such changes represent the final step toward plaque formation, necrotization, and rupture, with the establishment of atherosclerosis. Several polyphenol groups, such as apigenin [ 99 ], curcumin and luteolin [ 100 , 101 ], quercetin [ 102 ], epicatechin [ 103 ], and resveratrol [ 104 ], have been shown to reduce platelet aggregation through the aforementioned anti-inflammatory pathways and inhibition of adhesion molecules [ 105 ].…”

Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells

“…As a major inflammatory factor, TNF-α has been reported to mediate the interaction between endothelial cells and effector monocytes ( Liu and Tie, 2019 ). In addition, TNF-α also plays a vital role in atherosclerotic inflammatory progression through raise expression of adhesion molecule and activation of ECs ( Zhang et al, 2020 ). Adhesion molecules (e.g.…”

The Effect of Resveratrol on the Cardiovascular System from Molecular Mechanisms to Clinical Results

“…Zhang et al provided a new insight into the anti-inflammatory effect RES. RES can cause downregulation of leukocyte adhesion molecules (VCAM-1, ICAM-1 and E-selectin) by the inhibition of TNF-α-induced NF-κB activation [ 60 ]. Moreover, RES can activate the Nrf-2 pathway, leading to decreased expression of ICAM-1 and then the inhibition of monocyte adhesion [ 61 ].…”

“…Akt: protein kinase B; AMPK: AMP-activated protein kinase; COX: cyclooxygenase; Drp1: dynamin-related protein 1; GPx: glutathione peroxidase; GSH: glutathione; eNOS: endothelial nitric oxide synthase; HO-1: heme oxygenase-1; ICAM: intracellular cell adhesion molecule; JAK: Janus kinase; MAPK: mitogen-activated protein kinase; mTOR: mammalian target of rapamycin; NF-κB: nuclear factor kappa B; NOX: NADPH oxidases; PI3K: phosphatidylinositol 3-kinase; PGC-1α: peroxisome-proliferator-activated receptor gamma coactivator 1-alpha; SOD: superoxide dismutase; STAT: signal transducer and activator of transcription proteins; TGF-β: transforming growth factor; TLR4: toll-like receptor 4; VCAM: vascular cell adhesion molecule. References: [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 44 , 45 , 46 , 47 , 55 , 56 , 57 , 59 , 60 , 61 , 62 , 64 , 68 , 69 , 70 , 71 , 73 , 74 , 78 , 79 , 84 , 85 , 86 , 87 , 92 , 94 , 97 , 98 , 100 , 101 , 105 ]. …”