Resveratrol Attenuates Oxidative Stress in Chemotherapy Induced Acute Kidney Injury: An Experimental Rat Model

Abstract

OBJECTIVE:To investigate the anti-oxidative and anti-nephrotoxic effects of resveratrol in cisplatin induced nephrotoxic albino Wistar rats. METHODS:This quasi-experimental study was performed at Isra University, Hyderabad, Pakistan. Thirty male albino Wistar rats were divided into three g r o u p s : g r o u p -A ( c o n t r o l ) , g r o u p -B ( c i s p l a t i n ) a n d g r o u p -C (cisplatin+resveratrol). Biochemical [serum urea, creatinine and glutathione peroxidase (GPX)] and renal histomorphology was performed in all groups after 21 days of treatment. RESULTS:Difference in mean pre-and post-experimental body weight was observed in all three groups. Mean body weight decreased from 241.7±8.5 gm to 196.50±9.34 gm and from 237±7.4 gm to 207.2±6.56 gm in group-B and group-C respectively. In group-A; mean serum urea was 22.7±2.66 mg/dl, serum creatinine was 0.45±0.05 mg/dl and serum GPX was 1.44±0.13 ηg/ml. In group-B; mean serum urea level was 51±3.65 mg/dl, mean serum creatinine was 0.78±0.05 mg/dl and serum GPX was 0.85±0.11 ηg/ml. In group-C, mean serum urea level was 32.8±1.45 mg/dl, serum creatinine level was 0.41±0.09 mg/dl and serum GPX was 1.53±0.08 ηg/ml. In group-A, renal structure was intact, marked changes were observed in renal histology of group-B while group-C displayed less glomerular damage. The mean distance between visceral and parietal layers of Bowman's capsule was 69.34±0.87 µm in group-A, 216.5±1.32 µm in group-B while 102.22±1.65 µm in group-C. Areas of peritubular fibrosis and congestion were observed in groups B and C but less prominent in group-C compared with group-B. CONCLUSION:Resveratrol therapy is a potent anti-nephrotoxic regime showing promising results in chemotherapy induced nephrotoxicity and oxidative stress.

Nephroprotective Effects of L-Arginine against Chemotherapy Induced Acute Kidney Injury in Wistar Rats

“…17 In our experiment, administration of cisplatin was followed by a reduction in the weight of the experimental animals, which is consistent with previous findings. 3 The animals receiving Larginine therapy showed a much less reduction in their weights, which is consistent with the findings of Eshraghi-Jazi et al, who observed that arginine ameliorates cisplatin-induced weight loss in male rats. 18 In this study, there was an increase in the blood urea and creatinine of all the animals receiving cisplatin as compared with the controls.…”

“…16 In our study, histological examination of the kidneys showed clear evidence of cisplatin-induced nephrotoxic injury manifested as marked tubular cell necrosis, vascular congestion, and tubular dilatation, which is consistent with previous findings. 3 However, these changes were much less evident in the arginine treated animals. These findings are consistent with the findings of Başhan and coworkers, who noted that histopathological changes were reversed in the nephrotoxic animals who received L-arginine as well.…”

“…), nephrotoxic drugs including chemotherapeutic agents like Cisplatin, are the main causative factors, as demonstrated in 19% to 25% of cases. 3 Cisplatin is a very common and effective antineoplastic drug used against a wide range of cancers. Along with its effective use, it also poses many harmful effects on the tissues due to its reactive oxygen species (ROS) generating capability that causes depletion of glutathione levels and inhibition of renal antioxidant enzymes resulting in nephrotoxicity.…”

Protection of Resveratrol Against Nephrotoxicity in Rats Produced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin

“…nephroprotective [20], antiinflammatory [21], antidepressant and antioxidant properties [22][23][24]and effects of immunity enhancers W Egyptian Journal of Veterinary Sciences https://ejvs.journals.ekb.eg/ Egypt. J. Vet.…”