Resveratrol ameliorates experimental periodontitis in diabetic mice through negative regulation of TLR4 signaling

Abstract

Aim: To investigate the therapeutic effects of resveratrol (RSV) on periodontitis in diabetic mice and to explore the underlying mechanisms in vitro. Methods: Experimental periodontitis was induced in db/db mice by ligature application of porphyromonas gingivalis. The mice were treated with RSV (20 mg/kg, po) daily for 4 weeks. Alveolar bone loss, proinflammatory cytokines and TLR4 expression in the gingival tissue were measured. Cultured gingival epithelial cells (GECs) were used for in vitro studies. The transcriptional activity of TLR4 downstream signaling was analyzed using Western blotting. Results: RSV administration significantly decreased the blood glucose levels, and ameliorated alveolar bone loss in db/db mice with experimental periodontitis. RSV administration also suppressed the high levels of IL-1β, IL-6, IL-8, TNF-α, and TLR4 in gingival tissue of the mice. In the GECs incubated in high glucose medium, TLR4 expression was substantially upregulated, which was partly blocked in the presence of RSV. Lipopolysaccharides markedly increased the expression and secretion of IL-1β, IL-6, IL-8, and TNF-α in the GECs cultured in high glucose medium, which was also partly blocked in the presence of RSV. Furthermore, RSV significantly suppressed the phosphorylation of TLR4 downstream factors NF-κB p65, p38MAPK, and STAT3. Conclusion: RSV exerts protective effects against experimental periodontitis in db/db mice via negative regulation of TLR4 signaling.

Resveratrol attenuates oxidative stress during experimental periodontitis in rats exposed to cigarette smoke inhalation

“…Resveratrol mediated inhibition of CSI‐induced increases in bone loss seen in EP agree with previous studies done by our research group and others . Reduced periodontal damage related to use of resveratrol is high if not solely related to its effects on levels of IL‐17, IFN‐γ, IL‐4, and TH17/TH2 response .…”

Resveratrol Inhibits Periodontitis‐Related Bone Loss in Rats Subjected to Cigarette Smoke Inhalation

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Resveratrol derivative‐rich melinjo seed extract induces healing in a murine model of established periodontitis

“…It has been shown in an animal model of experimental periodontitis (EP) that resveratrol almost completely inhibits the progression of periodontal bone loss and decreases the levels of specific proinflammatory cytokines . In light of these findings, it has been suggested that resveratrol could conceivably prove to be a useful therapeutic agent for patients who have periodontitis.…”